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Genes involved in the regulation of starch metabolism and defense response determine resistance in citrus against huanglongbing

Research Paper | August 1, 2020

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Rozina Aslam, Iqrar Ahmad Khan, Muhammad Sarwar Yaqub

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Int. J. Biosci.17( 2), 121-129, August 2020

DOI: http://dx.doi.org/10.12692/ijb/17.2.121-129


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Different diseases and pests affect the genus citrus, resulting in reduced quality and taste of fresh fruit, low production and ultimately less profit for the growers. Huanglongbing (HLB) is the major problem for citrus-producing countries including Pakistan all over the world. Asian citrus psyllid (ACP) was collected from HLB positive sweet orange cv. succari (Citrus sinensis (L.) Osbeck) field trees and released on healthy plants of succari in the growth room under controlled conditions for the infestation to obtain bud/grafts for inoculation in experimental mandarin plants. SYBR green-based real-time qPCR was performed to differentiate the expression of carbohydrate metabolism and defense response-related selected genes in HLB infected and healthy leaf samples of kinnow, citrus sunki, parsons special and sun chu sha. Gene expression data analysis results represented that glucose-1-phosphate adenyl transferase, starch synthase and cytochrome P450 monooxygenase 83B1 genes may play a role in the tolerance against HLB.


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Genes involved in the regulation of starch metabolism and defense response determine resistance in citrus against huanglongbing

Albrecht U, Bowman KD. 2008. Gene expression in Citrus sinensis (L.) Osbeck following infection with the bacterial pathogen Candidatus Liberibacter asiaticus causing Huanglongbing in Forida. Plant Science 175, 291-306.

Albrecht U, Bowman KD. 2012. Transcriptional response of susceptible and tolerant citrus to infection with Candidatus Liberibacter asiaticus. Plant Science 185-186, 118-130.

Anonymous. 2017. FAO Statistical Year Book. Food and Agriculture organization of the United Nations 169.

Aslam R, Khan IA, Rahman KU, Asghar M. 2017a. Detection of ‘Candidatus Liberibacter asiaticus’ the causal organism of huanglongbing, in mandarin group of citrus. International Journal of Agriculture and Biology 19, 255‒258. http://dx.doi.org/10. 17957/IJAB/15.0271.

Aslam R, Khan IA, Rahman KU, Asghar M, Yaqub MS. 2017b. Expression profiling of huanglongbing disease in kinnow (Citrus reticulata) and succari (Citrus sinensis) leaves. International Journal of Agriculture and Biology 19, 1187‒1192. http://dx.doi.org/10.17957/IJAB/15.0409.

Aubert B. 1987. Trioza erytreae Del Guercio and Diaphorina citri Kuwayama (Homoptera:Psylloidea), the two vectors of citrus greening disease: biological aspects and possible control strategies. Fruits 42, 149-162.

Baldwin E, Plotto A, Manthey J, McCollum G, Bai J, Irey M, Cameron R. 2010. Effect of liberibacter infection (huanglongbing disease) of citrus on orange fruit physiology and fruit/fruit juice quality: Chemical and physical analyses. Journal of Agriculture and Food Chemistry 58, 1247-1262.

Bove JM. 2006. Huanglongbing: a destructive, newly emerging, century old disease of citrus. Journal of Plant Pathology 88, 7-37.

Chin E, Mishchuk DO, Bruce J, Cilia M, Coaker G, Davis C, Jin H, Ma W,  Sellar G, LeVesque C, Godfrey K, Slupsky CM. 2014. An interdisciplinary approach to combat HLB. CRB- funded research project report. Citrograph Magazine P 28-34.

Du D, Rawat N, Deng Z, Gmitter FG. 2015. Construction of citrus gene coexpression networks from microarray data using random matrix theory. Journal of Horticulture Resarch 15026. http://dx.doi.org/10.1038/hortres.2015.26

Duan Y, Zhou L, Hall DG, Li W, Doddapaneni H, Lin H, Liu L, Vahling CM, Gabriel DW, Williams KP. 2009.  Complete genome sequence of citrus huanglongbing bacterium, ‘Candidatus Liberibacter asiaticus’ obtained through metagenomics. Molecular Plant Microbe Interaction 22(8), 1011-1020.

Grennan AK. 2006. Regulation of starch metabolism in Arabidopsis. Plant Physiology 142(4), 1343–1345.

Hocquellet A, Toorawa P, Bove JM, Garnier M.1999. Detection and identification of two Candidatus liberobacter species associated citrus huanglongbing by PCR amplification of ribosomal protein genes of the beta operon. Molecular and Cellular Probes 13, 373-379.

Jagoueix S, Bove JM, Garnier M. 1996. PCR detection of two candidatus Liberibacter species associated with greening disease of citrus. Molecular and Cellular Probes. 10, 43-50.

Kim JS, sagaram US, Burns JK, Li JL, Wang N. 2009. Response of sweet orange (Citrus sinensis) to Candidatus Liberibacter asiaticus infection: Microscopy and microarray analyses. Phytopathology 99, 50-57.

Li W, Hartung JH, Levy L. 2006. Quantitative real time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing. Journal of Microbiological Methods 66, 104-115.

Li W, Levy L, Hartung JH. 2009. Quantitative distribution of ‘Candidatus Liberibacter asiaticus’ in citrus plants with citrus huanglongbing. Phytopathology 99, 139-144.

Liao HL, Burns JK. 2012. Gene expression in citrus sinensis fruit tissues harvested from huanglongbing-infected trees: comparison with girdled fruit. Journal of Experimental Botany 63(8), 3307-3319.

Lin H, Pietersen G, Han C, Read DA, Lou B, Gupta G, Civeroloa EL. 2015. Complete genome sequence of “Candidatus Liberibacter africanus,” a bacterium associated with citrus huanglongbing. Genome Announcements 3(4). http://dx.doi.org/10.1128/GenomeA.00733-15.

Liu YH, Tsai JH. 2000. Effects of temperature on biolology and life table parameters of the Asian citrus psyllid, Diaphorina citri Kuwayama (Homoptera: Psyllidae). Annals of Applied Biology 137(3), 201-206.

Martinelli F, Ibanez AM, Reagan RL, Davino S, Dandekar AM. 2015. Stress responses in citrus peel: Comparative analysis of host responses to huanglongbing disease and puffing disorder. Scientia Horticultureae 192, 409-420.

Martinelli F,  Uratsu SL, Albrecht U, Reagan RL, Phu ML, Britton M, Buffalo V, Fass J, Leicht E, Zhao W, Lin D, Souza RD, Davis CE, Bowman KD, Dandekar AM. 2012. Transcriptome profiling of citrus fruit response to huanglongbing disease. PLoS ONE 7(5), 1-16.

Nwugo CC, Duan Y, Lin H. 2013. Study on Citrus response to huanglongbing highlights a down-regulation of defense-related proteins in Lemon plants upon ‘Ca. Liberibacter asiaticus’ infection. PLoS ONE 8(6), e67442. http://dx.doi.org/10.1371/journal.pone.0067442

Pfaffl MW. 2001. A new mathematical model for relative quantification in real-time RT- PCR. Nucleic Acid Research 29(9), 2002-2007.

Ruangwong O, Akarapisan A. 2006. Detection of Candidatus Liberibacter asiaticus causing Citrus Huanglongbing disease. Journal of Agriculture Technology 2(1), 111-120.

Sambrook J, Russel DW. 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.

Staiger CJ, Baluska F, Volkmann D, Barlow P. 2000. Actin: A Dynamic for Multiple Plant Cell Functions. Plant Science, p 669.

Tahir A. 2014. Forecasting citrus exports in Pakistan. Pakistan Journal of Agricutural Science 27(1), 64-68.

Vandesompele J, Preter, KD, Pattyn F, Poppe B, Roy NV, Paepe AD, Speleman F. 2002.  Accurate normalization of real time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3(7), 1-12.

Wulff NA, Zhang S, Setubal JC, Almeida NF, Martins EC, Harakava R, Kumar D, Rangel LT, Foissac X, Bove JM, Gabreil DW. 2014. The complete genome sequence of ‘Candidatus Liberibacter americanus’, associated with citrus huanglongbing. Molecular Plant-Microbe Interactions 27(2), 163–176.

Yaqub MS, Khan IA, Aslam R. 2019. Asian citrus psyllid (diaphorina citri kuwayama) rearing for transmission of Candidatus liberibacter asiaticus in citrus for the management strategies of huanglongbing, Pakistan Entomologist 41, 63-71.

Yaqub MS, Khan IA, Usman M, Rana IA. 2017. Molecular detection of Candidatus Liberibacter asiaticus, the causal organism of huanglongbing (citrus greening) in Faisalabad, Pakistan for huanglongbing management. Pakistan Journal of Agricultural Sciences 54, 21-26. http://dx.doi.org/10.21162/PAKJAS/17.4455.