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Genetic characterization of Berberis species collected from Kunhar River catchment using morphological and molecular markers

Ajmal Iqbal, Israr Ahmad, Habib Ahmad, Sajidul Ghafoor, Abdul Razzaq, Istiak Ahmed

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Int. J. Biosci.3(5), 35-42, May 2013

DOI: http://dx.doi.org/10.12692/ijb/3.5.35-42

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Abstract

The Berberis genus represents perennial spiny shrubs with reddish brown to pale yellowish stem bark. From Pakistan Berberis have been classified on morphological characters but no attempt was made to classify it on molecular traits. The first documented report of using molecular as well as numerical parameters for dissecting the genus from different localities of Kunhar River catchment. The assessment of Berberis is genus based upon 19 morphological characters. The data obtained from numerical analysis was computed for getting dendrogram, which classified 24 collections into two major groups i.e. group A and B. All the genotypes of group-A was having red fruits whereas the collections clustered into group-B produce black fruits. Molecular characterization was done with the help of 12 RAPD primes to elaborate genetic polymorphism in Berberis colletions. The primers based amplification in the collections revealed high level of genetic polymorphism, i.e. 6-86%. The genetic diversity estimated as genetic distances computed into dendrogram, separated the collections into 5 distinct groups A, B, C, D and E, disseminated as Berberis parkeriana, B. lycium, B. pachyacantha, B. kunwarensis and B. orthobotrys, respectively. However the collection B-8, which closely resembles B. parkeriana morphology was out grouped and needs future elaboration with more markers assorted discrimination.

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Genetic characterization of Berberis species collected from Kunhar River catchment using morphological and molecular markers

Ahmad S. 1969. Contributions to the fungi of Pakistan. IX. Biologia 15, 1-10.

Ajmone MP, Catiglioni P, Fusari F, Kuiper M, Maotto M. 1998. Genetic diversity and its relationships to hybrid performance in maize as revealed by RFLP and AFLP markers. Theoretical and Applied Genetics 96, 602-611. http://dx.doi.org/10.1007/s001220050730

Ayele M, Tefera H, Assefa K, Nguyen HT. 1999. Genetic characterization of two Eragrostis species using AFLP and morphological traits. Hereditas 130, 33-40. http://dx.doi.org/10.1111/j.1601-5223.1999.00033.x

Bottini MCJ, Bustos AD, Sanso AM, Jouve N, Poggio L. 2007. Relationships in Patagonian species  of  Berberis  (Berberidaceae)  based  on  the characterization of rDNA internal transcribed spacer sequences. Botanical Journal of the Linnean Society 153, 321–328. http://dx.doi.org/10.1111/j.1095-8339.2007.00586.x

Bottini MCJ, Bustos AD, Jouve N, Poggio L. 2002. ALFP characterization of natural populations of Berberis (Berberidaceae) in Patagonia,Argentina. Plant Systematics and Evolution 231, 133-142. http://dx.doi.org/10.1007/s006060200015

Bottini MCJ, Premoli AC, Poggio L.1999 b. Hybrid speciation in Berberis L. An isoenzymatic approach. In: XVI International Botanical Congress, St. Louis, Missouri, USA.

Caroso SRS, Eloy NB, Provan J, Caroso MA, Ferreira PCG. 2000. Genetic differentiation of Euterpeedulis Mart. Population estimation by AFLP analysis. Molecular Ecology 9, 1753-1760. http://dx.doi.org/10.1046/j.1365-294x.2000.01056.x

Cronquist A. 1981. An integrated system of classification of flowering plants. New York: Columbia Univ. Press. ISBN: 0231038801, 9780231038805.

Doyle JJ, Doyle JL. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13-15.

Ghafoor A, Sharif A, Ahmad Z, Zahid MA, Rabbani MA. 2001. Genetic diversity in Blackgram (Vignamungo) Field Crops Research 69, 183-190. http://dx.doi.org/10.1016/S0378-4290(00)00141-6

Jafri SMH. 1975. Berberidaceae. In E. Nasir and S. I. (edt.) Flora of West Pakistan.Department of Botany, Karachi University, Karachi. Pakistan 87, 1-31.

Jain A, Bhatia S, Banga SS, Prakash S, Laxmikumaran M. 1994. Potential use of RAPD technique to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theoretical and Applied Genetics 88, 116–122. http://dx.doi.org/10.1007/BF00222403

Jan S, Khan MA, Din S, Murad W, Hussain M, Ghani A. 2008. Herbal Rmidies used for Gastrointestinal Disorders in Kaghan Valley, NWFP, Pakistan. Pakistan Journal of Weed Science Research 14, 169-200.

Lerceteau  E,  Szmidt  AE.  1999.  Properties  of AFLP markers in the inheritance and genetic diversity studies of Pinus sylvestris L. Heredity 82, 252-260. http://dx.doi.org/10.1038/sj.hdy.6884720

Lodhi MA, Guang-Ning Y, Norman FW, Bruce IR. 1994. A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Ampelopsis. Plant Molecular Biology Reporter 12, 6-13. http://dx.doi.org/10.1007/BF02668658

Mackill DJ. 1995. Classifying japonica rice cultivars with RAPD markers. Crop Science 35, 889–894. http://dx.doi.org/10.2135/cropsci1995.0011183X00 3500030043x

Mueller UG, Wolfenbarger L. 1999. AFLP genotyping and fingerprinting. Trends in Ecology & Evolution 14, 389–394. http://dx.doi.org/10.1016/S0169-5347(99)01659-6

Naghavi MR, Jahansouz MR. 2005. Variation in the agronomic and morphological traits of Iranian chickpea  accessions.  Journal  of  Integrative  Plant Biology 47, 375-379. http://dx.doi.org/10.1111/j.1744-7909.2005.00058.x

Nei M, Li WH. 1979. Mathematical for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of sciences of the United States of  America 76, 5269-5273. http://dx.doi.org/10.1073/pnas.76.10.5269

SPSS Inc. 1996. SPSS 13.5 for windows 1996. SPSS, Inc, Chicago.

Stewart RR. 1967. Check list of the plants of Swat State, Northwest Pakistan. Pakistan Journal of Forestry 4, 457-528.

Stewart RR. 1972. An Annotated Catalogue of Vascular Plants of West Pakistan and Kashmir. Fakhri Printing Press, Karachi.

Sultana T, Ghafoor A, Ashraf M. 2006. Geographical patterns of diversity of cultivated lentil germplasm collected from Pakistan, as assessed buy seed protein assays. Acta Biologica Cracoviensia, Series Botanica, Poland 48, 77-84.

Takhtajan AL. 1987. Systemamagnoliophytorum. Leningrad. Nauka, Leningrad

Tosto DS, Hopp HE. 2000. Suitability of AFLP markers for the study of genomic relationships within the Oxalis tuberosea Alliance. Plant Systematics and Evolution 223, 201-209. http://dx.doi.org/10.1007/BF00985279

Xi-ven, L. and I. C. Hedge. 1994. Flora of China. Science Press Beijing 17, 237-239.

Yu  KF,  Pauls  KP.  1993.  Rapid  estimation  of genetic relatedness among heterogeneous populations of alfa-alfa by random amplification of bulked genomic DNA samples. Theoretical and Applied Genetics 86, 788–794.

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