Genetic characterization of Biomphalaria arabica, the molluscan intermediate host for schistosomes in Saudi Arabia

Paper Details

Research Paper 01/11/2012
Views (196) Download (1)

Genetic characterization of Biomphalaria arabica, the molluscan intermediate host for schistosomes in Saudi Arabia

Najat Hamed, Ramesa Shafi, Nada Al-Tassan, Sooad Al-Daihan
Int. J. Biosci.2( 11), 30-36, November 2012.
Certificate: IJB 2012 [Generate Certificate]


The present study was a comparative analysis of genetic variability of Biomphalaria arabica, the intermediate hosts of Schistosoma mansoni from two different areas having different prevalence level of Schistosomiasis in Saudi Arabia. Biomphalaria arabica samples from Riyadh and Hofuf area were studied at the genotypic level by RAPD analysis in an attempt to associate possible polymorphic markers related to susceptibility to infection by the Schistosoma mansoni. A total of 9 primers were tested and polymorphic markers were obtained. The results showed polymorphism with 3 primers and suggested that RAPD represents an efficient means of genome comparison, since many molecules markers were detected as genetic variations between Riyadh and Hofuf snails. The present study confirms that genetic profile of Biomphalaria arabica is critically important for the success of schistosome life cycle and that snail host plays at least a part in prevalence of the disease.


Abdel-Hamid AH, de Molfetta JB Fernandez V, Rodrigues V. 1999. Genetic variation between susceptible and non-susceptible snails to Schistosoma infection using random amplified polymorphic DNA analysis (RAPDs). Rev. Inst. Med. Trop., São Paulo 41, 291–295.

Abdel-Hamid AH, Rawi SM, Arafa AF. 2006. Identification of a genetic marker associated with the resistance to Schistosoma mansoni infection using random amplified polymorphic DNA analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro 101, 863-868.

Abahussain A. 2005. Prevalence of intestinal parasites among expatriate workers in Al-Khobar, Saudi Arabia. Middle East Journal of Family Medicine. 3, 17-21.

Arfaa F. 1976. Studies on schistosomiasis in Saudi Arabia .Am J Trop Med Hyg.25 295-298.

Ashi J. 1989. Progress achieved in the control of schistosomiasisin Saudi Arabia. Tropic Medicine and Hygiene 92, 27-31.

Barral V, This P, Imbert- Establet D, Combes C, Delseny M. 1991. Genetic variability and evaluation of Schistosoma genome analyzed by using random amplified polymorphic DNA markers. Molecular and Biochemical Parasitology, 59, 211-222.

Chitsulo DE, Montresor A, Savioli L. 2000. The global status of schistosomiasis and its control, Acta Trop. 77, 41–51.

Coelho PM, Carvalho OS. Andrade ZA, Martins-Sousa RL, Rosa FM. 2004. Biomphalaria tenagophila/Schistosoma mansoni interaction: Premises for a new approach to biological control of schistosomiasis. Mem. Inst. Oswaldo Cruz 99, 109-111.

Dice LR. 1945. Measures of the amount of ecological association between species. Ecology 26. 297–302.

El-Khayat HM, Abu El Einin H M. and Gawish FA. 2008. Determination of allozyme, protein and schistosome susceptibility in Biomphalaria alexandrina progenies produced by self and cross fertilization, Egypt. Soc. Parasitol. 38(3), 1007–1024.

Knight M, Miller AN, Patterson CN, Rowe C G, Michaels G, Carr D, Richards CS. Lewis FA. 1999. The identification of markers segregating with resistance to Schistosoma mansoni infection in the snail Biomphalaria glabrata, Proc. Natl. Acad. Sci. U.S.A. 96, 1510–1515.

Mulvey M, Woodruff DS. 1985. Genetics of Biomphalaria glabrata: linkage analysis of genes for pigmentation, enzymes, and resistance to Schistosoma mansoni, Biochem. Genet. 23(11–12), 877–889.

Nabih  I,  El  Dardire  Z.  and  El-Ansary  A. 1989.Selected enzymatic activities in freshwater snail-specific intermediate hosts for human Schistosomiasis . Cell. Molec. Biol. 35, 181-186.

Oliveira AL, Silva D Da , Zanotti-Magalhaes EM , Abdel-Hamid AZ, Ribeiro-Paes JT. 2008. Schistosome/mollusk: genetic compatibility, Genet. Mol. Res. 7(2), 518–526.

PerringTM, AD Cooper, RJ Rodriguez, CA Farrar, and TS Bellows Jr. 1993a. Identification of a Whitefly Species by Genomic and Behavioral Studies. Science 259, 74-76.

Pillay D, Pillay B. 1994. Random amplified polymorphic DNA analysis shows intraspecies variation among Schistosoma mansoni isolates. Med Sci Res. 22, 369-71.

Rollinson D, Stothard JR. 1998. Molecular characterisation of intermediate snail hosts and the search for resistance genes. Mem Inst Oswaldo Cruz 1, 111-116.

Simpson AJG, Neto ED & Steindel M. 1993. The use of RAPDs for the analysis of parasites. In: PENA SDJ, CHAKRABORTY R, EPPLEN LT & JEFFREYS J (eds), DNA fingerprinting: state of the sciences. Boston, Birkhauser. 331-337.

Spada RG, Da Silva D. 2002. Genetic markers between Biomphalaria glabrata snails susceptible and resistant to Schistosoma mansoni infection. Mem Inst Oswaldo Cruz 97, 53-8.

Tanka H. and Tsuji. 1997. From discovery to eradication of schistosomiasis in Japan:1847-1996.Int. J. Parasitol. 27, 1465-1480.

Vidigal  TA,  Dias  Neto  E, Carvalho OS & Simpson AJG. 1994. Biomphalaria glabrata: Extensive genetic variation in Brazilian isolates revealed by random amplified polymorphic DNA analysis. Experimental Parasitology 79, 187-194

Welsh J, McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18, 7213-7218.

WHO. 2010. Schistosomiasis Fact Sheet,. No.115.

Williams JA, Scott IM , Atkin AL, Brook WJ, Russell MA, Bell JB. 1990. Genetic and molecular analysis of vgU and vgW: two dominant vg alleles associated with gene fusions in Drosophila. Genetics 125, 833-844.