Paper Details

Genetic characterization of local chicken from Taif region in Saudi Arabia using RAPD markers

Amena M. Ibrahim,Ayman M.Sabry,Mohamed M.Hassan,Ehab I. El-Hallous,Alaa A. Mohamed

DOI: https://dx.doi.org/10.12692/ijb/6.10.142-148

Int. J. Biosci. 6(10), 142-148. May, 2015. (PDF)

Abstract:

The Taif domestic chicken (Gallus gallus, 2n= 78) is believed to have descended from the wild Indian and Southeast Asian red jungle fowl. The molecular characterization of local chickens in different districts of Taif region in Saudi Arabia was done using RAPD markers of the variable region. The twelve random primers were used to generate fingerprint patterns for these chickens. Twenty five DNA of blood samples from individual chicken were tested to confirm the fingerprinting and genetic distance among these chickens. Specific results for fingerprinting were obtained by the twelve primers retained for RAPD analysis produced different fragment patterns with varied number of bands. The primers yielded a total of 187 distinct bands 34.7% were considered as polymorphic bands and 65.3% were considered as monomorphic bands. The OPA-06 primer has showed the highest polymorphism 83.3% and total of 18 bands ranged from 250 bp-1700 bp. While, the OPA-03 primer has showed the lowest polymorphism 7.8% and total of 14 bands ranged from 200 bp-1650bp.The Dendrogram based on RAPD results grouped the twenty five individual local chicken samples into two different clusters with about 80% genetic similarity. Moreover, the genetic distance among native chicken was relatively low. The smallest genetic distance (0.09) was estimated between sample No. 23 and sample No. 19.This work aims to estimate the genetic resources in the local Saudi chickens reared using RAPD markers in Taif region as preliminary work to established the basis for genetic conservation program for local chicken in Saudi Arabia.