Genetic diversity and heritability of chlorophyll content and photosynthetic indexes among some Iranian wheat genotypes

Paper Details

Research Paper 01/01/2014
Views (304) Download (7)

Genetic diversity and heritability of chlorophyll content and photosynthetic indexes among some Iranian wheat genotypes

M. Khodadadi, H. Dehghani, M.H. Fotokian, B. Rain
J. Bio. Env. Sci.4( 1), 12-23, January 2014.
Certificate: JBES 2014 [Generate Certificate]


Genetic diversity and heritability of Chlorophyll (Chl) content and some photosynthetic indexes were studied in thirty wheat genotypes through randomized complete block design with three replications at 2009. The maximum and minimum heritability were obtained for chlorophyll (0.75-0.89) and stomatal conductance (0.2), respectively. The least phenotypic and genotypic coefficients of variation were observed for CO2 levels in boundary layer. Phenotypic and genotypic correlation coefficients between Chl a, Chl b and total Chl were similar and between SPAD Chl and Chl b were significantly high. Chl b content and transpiration rate, stomatal conductance and H2O levels in boundary layer had positive and significant genotypic correlation. CO2 levels in boundary layer and stomatal conductance were significantly and linearly associated with photosynthesis rate. 78.62% of data variation was explained by two factors. Genotypes in first group showed appropriate photosynthetic characters for resistance to drought and heat. Genotypes in third group have high chlorophyll content and photosynthesis rate. Maximum (103.19) and minimum (0.98) genetic distance was found between Moghan 3 and Kavir and between star and Tajan, respectively.


Ariyo OJ. 1995. Component analyses and their implication on the breeding of soya bean (Glycine max L. Merr). Pertanika Journal of Tropical Agricultural Science 18(3), 201-207.

Awan SI, Niaz S, Malik MFA, Ali S. 2007. Analysis of variability and relationship among seedling traits and plant height in semi-dwarf wheat (Triticum aestivum L.). Journal of Agriculture and Social Sciences 3(2), 59-62.

Berghaus RD, Lombard JE Gardner IA, Farver TB. 2005. Factor analysis of a johne’s disease risk assessment questionnaire with evaluation of factor scores and a subset of original questions as predictors of observed clinical paratuberculosis. Preventative Veterinary Medicine 72, 291-309.

Bhatt RK, Baig MJ, Tiwari HS, Roy S. 2010. Growth, yield and photosynthesis of Panicum maximum and Stylosanthes hamata under elevated CO2. Journal of Environmental Biology 31, 549-552.

Bojovic B, Stojanovic j. 2005. Chlorophyll and Carotenoid Content in Wheat Cultivars as a Function of Mineral Nutrition. Archives of Biological Sciences, Belgrade 57, 283-290.

Burton GW. 1952. Quantitative Inheritance in grasses, Proceeding of 6th International Grassland Congress. National Publishing Company, 17-23 Aug 1952. Washington, D.C. USA, p. 277-283.

Carver BF, Nevo E. 1990. Genetic diversity of photosynthetic characters in native populations of Triticum dicoccoides. Photosynthesis Research 25, 119-128.

Del Blanco IA, Rajaram S, Kronstad WE, Reynolds MP. 2000. Crop physiology & metabolism; physiological performance of synthetic hexaploid wheat-derived populations. Crop Science 40, 1257–1263.

Denis JC, Adams MW. 1978. A factor analysis of the plant variables related to yield in dry beans; Morphological traits. Crop Science 18, 74-78.

Dewey JR, Lu KH. 1956. A correlation and path coefficient analysis and components of crested wheat seed production. Journal of Agronomy 51, 515-518.

Garson D. 2004. Factor Analysis. m

Ghain BS, Srivastava AK, Gill KS. 1969. Inheritance of amount of chlorophyll in wheat, Triticum aestivum L. Euphytica 18, 403-405.

Gohari AM, Sedaghat N, Javan nikkhah M, Saberi-Riseh R. 2007. Mycoflora of Wheat Grains in the Main Production Area in Kerman Province, Iran. International Journal of Agriculture and Biology 9(4), 635-637.

Havaux M, Tardy F. 1999. Loss of Chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high drought and heat stress. Australian Journal of Plant Physiology 26, 569–578.

Johnson  HW,  Robinson  HF,  Comstock  RE. 1955. Estimates of genetic and Environmental variability in Soybeans. Agronomy Journal 47(7), 314-318.

Jordaan H, Grové B. 2007. Factors affecting maize producers adoption of forward pricing in price risk management: The Case of Vaalharts Agrekon 46(4), 548- 565.

Joshi AB, Dhawan NL. 1966. Genetic improvement of yield with special reference to self-fertilizing crops. Indian Journal of Genetics and Plant Breeding 26, 101-113.

Joshi BK, Mudwari A, Bhatta MR, Ferrara GO. 2004. Genetic diversity in Nepalese Wheat Cultivars Based on Agro-Morphological Traits and Coefficients of Parentage. Nepal Agriculture Research Journal 5, 7-17.

Kapotis G, Zervoudakis G, Veltsistas T, Salahas G. 2002. Comparison of Chlorophyll Meter Readings with Leaf Chlorophyll Concentration in Amaranthus vlitus: Correlation with Physiological Processes. Russian journal of plant physiology 50(3), 395-397.

Kashif M, Khaliq I. 2004. Heritability, correlation and path coefficient analysis for some metric traits in wheat. International Journal of Agriculture and Biology 6(1), 138-142.

Kof EM, Oorzhak AS, Vinogradova IA, Kelibernaya ZV, Krendeleva TE, Kukarskikh ZV, Kondykov IV, Chuvasheva ES. 2004. Leaf morphology pigment complex, and productivity in wild type and alpha Pea genotypes. Russian Journal of Plant Physiology 51, 449-454.

Kura Hotta M, Satoh K, Katoh S. 1987. Relationship between photosynthesis and chlorophyll content during leaf senescence of rice seedlings. Plant and Cell Physiology 28, 1321-1329.

Lichtenthaler HK, Wellburn AR. 1985. Determination of total carotenoids and chlorophylls a and b of leaf in different solvents. Biological Society of Trans 11, 591-592.

Mebrahtu T, Hanover JW, Layne DR, Flore JA. 1991. Leaf temperature effects on net photosynthesis, dark respiration, and photorespiration of seedlings of black locust families with contrasting growth rates. Canadian Journal of Forestry Research 21, 1616-1621.

Miah MNH, Yoshida T, Yomamoto Y. 1997. Effect of nitrogen application during ripening period on photosynthesis and dry matter production and its impact on yield and yield components of semi dwarf indica rice varieties under water culture conditions. Soil Science and Plant Nutrition 43(1), 205-217.

Mishra RS, Lotha RE, Mishra SN, Paul PK, Mishra HN. 1988. Results of heterosis breeding on chilli (Capsicum annuum L.). Capsicum Newsletter 7, 49-50.

Mohammadi M, Karimizadeh RA, Naghavi MR. 2009. Selection of Bread Wheat Genotypes against Heat and Drought Tolerance Based on Chlorophyll Content and Stem Reserves. Journal of Agriculture and Social Sciences 5, 119–122.

Mohammadi SA, Prasanna BM. 2003. Analysis of genetic diversity in crop plants: salient statical tools and considerations. Crop Science 43, 1235-1248.

Peri PL, Mootb DJ, Mcneilb DL, Lucasb RJ. 2003. Modelling net photosynthetic rate of field-grown cocksfoot leaves to account for regrowth duration. New Zealand Journal of Agricultural Research 46, 105-115.

Rebetzke GJ, Condon AG, Richards RA. 1996. Rapid screening of leaf conductance in segregating wheat populations. In: Richards R, Wrigley C, Rawson H, Davidson J and Brettell R I (Eds). Proceedings of the Eighth Assembly of the Wheat Breeding Society of Australia Canberra: Wheat Breeding Society of Australia, Australia. Pp. 130–134. Saleem U, Khaliq I, Tariq M, Rafique M. 2006. Phenotypic and genotypic correlation coefficients between yield and yield components in wheat. Journal of Agricultural Research 44, 1-8.

Singh RK, Chowdhury BD. 1985. Biometrical method in quantitative genetic analysis Kalyani publishers, Ludhiana, New Delhi.

Singh SP. 2001. Broadening the genetic base of common bean cultivars. Crop Science 41, 1659-1675.

Solomon KF, Labuschagne MT. 2003. Variation in water use and transpiration efficiency among durum wheat genotypes grown under moisture stress and non-stress conditions. Journal of Agricultural Science 144(2), 31-41.

Steel RGD, Torrie JH. 1984. Principles and Procedures of Statistics. A Biometrical Approach New York, McGraw Hill Book Co.

Thomas JA, Jeffrey AC, Atsuko K, David MK. 2005. Regulating the proton budget of higher plant photosynthesis. Proceedings of the National Academy of Sciences USA 102, 9709–9713.

Toker C, Cagirgan MI. 2003. Selection criteria in chickpea (Cicer arietinum L.). Acta Agriculturae Scandinavica Section B-Soil and Plant Science 53, 42–5.

Toker C, Cagirgan MI. 2004. The use of phenotypic correlation and factor analysis in determining characters for grain yield selection in chickpea (Cicer arietinum L.). Heriditas 140, 226–8.

Tulio B, David K, Weaver, Robert KD. 2007. Photosynthesis in wheat at the grain filling stage is altered by larval wheat stem sawfly (Hymenoptera: Cephidea) injury and reduced water availability. Journal of Entomological Science 42, 228-238.

Zhang K, Zhang Y, Chen G, Tian J. 2009. Genetic analysis of grain yield and leaf chlorophyll content in common wheat. Cereal Research Communications 37(4), 499–511.