Genetic diversity and inter-relationship of henbane populations of North-West of Iran based on IRAP and REMAP markers

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Genetic diversity and inter-relationship of henbane populations of North-West of Iran based on IRAP and REMAP markers

Alireza Asghari Mirak, Seyed Siamak Alavi-Kia, Seyed Abolghasem Mohammadi, Daryush Talei
J. Bio. Env. Sci.16( 3), 18-28, March 2020.
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Henbane is one of the indigenous plants of Solanaceae from Iran that considered as valuable medicinal and economic plant. In current study, genetic diversity of 96 genotypes from 10 populations including three species was assessed using IRAP and REMAP markers. The employed primers included eight complimentary sequences of LTR regions of barley genome accompanied with eleven 3’-anchored ISSR primers. In the IRAP system seven out of the 36, and in the REMAP technique, 12 out of 88 primer combinations resulted in appropriate amplifications and scorable bands. The average PICs for IRAP and REMAP markers were accounted 0.30 and 0.32, and the mean MIs were 2.59 and 2.47, respectively. Cluster analysis using REMAP markers resulted in a phylogenetic tree with three distinct clusters which somewhat confirmed with geographical distribution of species, unlike the clustering by IRAP data. ANOVA using IRAP and REMAP data showed the higher variation within populations comparing with among populations. However, the acceptable diversity was observed within and among populations based on both marker systems.


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