Genetic Diversity in Egyptian Populations of Tilapia species Using RAPD and SRAP Markers
Paper Details
Genetic Diversity in Egyptian Populations of Tilapia species Using RAPD and SRAP Markers
Abstract
This study aimed to estimate the genetic diversity among and within populations of three Tilapia species e.g., Oreochromis niloticus, Tilapia zilli and Oreochromis aurea which were collected from different locations i.e., Ryad, Motobs (Kafr El Sheikh Governorate), and Bahr El Baqar (Sharqia Governorate).Random amplified polymorphic DNA (RAPD) and sequence related amplified polymorphism (SRAP) were used in this study. Our results indicated that percentages of polymorphism were 58.41, 41.87 and 31.49% according to RAPD whereas 68.96, 33.33 and 17.64% terms of SRAP for O. niloticus, T. zilli and O. aurea respectively. The percentages of polymorphism using RAPD and SRAP within O. niloticus populations were the highest values . Population from Ryad was closer to population of Bahr El Baqar in O. niloticus, while population of T. zilli and O. aurea from Bahr El Baqar was more similar to population from Motobs. The outcome of this study considered as baseline of genetic diversity which will be useful in breeding program and conservation biology of Tilapia species and a baseline analysis of the population differentiation found among of Tilapia species in Egypt.
Abd el-kader H, Abd el hamid Z, Mahrous K. 2013. Genetic diversity among three species of Tilapia in Egypt detected by Random Amplified Polymorphic DNA marker . Journal of Applied Biological Science.(7), 57-64.
Abdel-hamid Z, Abdel-kader H, Aboelhassan D, Mahrous K. 2014. Genetic diversity in Egyptian Tilapia species using PCR-RFLP of D-loop Mitochondrial DNA gene. Research Journal of Pharmaceutical, Biological and Chemical Sciences5,469-475.
Abdul Rahim M, Ismail P, Alias R, Muhammad N, Jais A. 2012. PCR-RFLP analysis of mitochondrial DNA cytochrome b gene among Haruan (Channastriatus) in Malaysia. Elsevier494, 1–10.
Ahmed MMM, Ali BA, EL-Zaeem SY. 2004. Application of RAPD markers in fish: Part I- some genera (Tilapia ,Sarotherodon and Oreochromis) and species (Oreochromisaureus and Oreochromisniloticus) of Tilapia. International Journal of Biotechnology 6(1), 86-93.
Ali B, Huang T, Qin D , Wang X. 2004.A review of random amplified polymorphic DNA (RAPD) markers in fish research. Reviews in Fish Biology and Fisheries. 14,443-453.
Bardakci F. 2000. The Use of Random Amplified Polymorphic DNA (RAPD) Markers in Sex Discrimination in Nile Tilapia ,Oreochromisniloticus (Pisces: Cichlidae). Turkish Journal of Biology 24,169–175.
Bhassu S, Yusoff K, Panandam M, Embong WK, Oyyan S, Tan SG. 2004. The genetic structure of Oreochromis spp. (Tilapia ) populations in malaysia as revealed by microsatellite DNA analysis. Biochemical Genenetics42,217-229.
Carleton KL, Streelman JT, Lee BY, Garnhart N, Kidd M, Kocher TD. 2002. Rapid isolation of CA microsatellites from the Tilapia genome. Animal Genetics 33, 140-144.
Chandra G, Saxena A, Barat A. 2010. Genetic diversity of two river in populations of Eutropiichthysvacha (Hamilton, 1822) using RAPD markers and implications for its conservation. Journal of Cell and Molecular Biology 8, 77-85.
Chauhan1 T, Rajiv K. 2010.Molecular markers and their applications in fisheries and aquaculture. Advances in Bioscience and Biotechnology1, 281-291.
Chen S, Dai T, Chang Y, Wang S, Ou S, chuang W, Cheng C, Lin Y, Lin L , Ku H. 2013. Genetic diversity among Ocimum species based on ISSR, RAPD and SRAP markers. Australian Journal of Crop Science7, 1463-1471.
Deng L, Qin R, Gao J, Jia Y, Ren Y, GaoW, Lu L.2013. SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe (+) implantation. Genetics and Molecular Research 12,335-343.
Ding WD, Cao ZM, Cao LP. 2010.Molecular analysis of grass carp (Ctenopharyngodon idella) by SRAP and SCAR molecular markers. AquacultInt 18, 575–587
Eshak M, Ebeed N, Booles H , Fahmy K. 2010. Genetic assessment of salinity tolerance in Red Tilapia. New York Science Journal3, 132-141.
Hassanien H, Elnady M, Obeida A, Itriby H. 2004. Genetic diversity of Nile Tilapia populations revealed by randomly amplified polymorphic DNA (RAPD). Aquaculture Research. 35,587-593.
Islam MS, Alam MA. 2004.Randomly amplified polymorphic DNA analysis of four different populations of the Indian major carp, Labeorohita (Hamilton). Journal of Applied Ichthyology 20,407-412.
Kocher T,LeeW,Sobolewska H, Penman D, Andrew B. 1998. A Genetic linkage map of a cichlid fish, the Tilapia (Oreochromis niloticus). Genetics, 148, 1225-1232.
Li G, Quiros C.2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics. 103,455-461.
Li W, Zhang J, Mou Y, Geng J, McVetty P, Hu S, Li G. 2011. Integration of Solexa sequences on an ultradense genetic map in Brassica rapa L. Bio-Medical Central Genomics 12,1-14
Li X , Li J, Zhao Z, Yang F, Fu Q, Liu H, Wang D, Yang Y, Wang R. 2014. Sequence-related amplified polymorphism (SRAP) for studying genetic diversity and population structure of plants and other living organisms: a protocol. The Journal of Animal & Plant Sciences 24,1478-1486.
Mojekwu TO, Oguntade OR, Oketoki TO, Usman AB, Omidiji O. 2013. Molecular characterization of Tilapia in different water bodies using RAPD markers. African Journal of Applied Biotechnology Research 1, 1-12.
Rashed M, Saad Y, Atta A, Ahmed N. 2011.Reconstruction of phylogenetic relations among four Tilapia species. World Applied Sciences Journal14,456-462.
Rashed M, Saad y, EL – SeoudyA, Ibrahim M. 2009. Gene flow in some Oreochromis niloticus populations based on SSR linked markers to MHC loci class I. Boilogicai and Chemical Environmental Sciences. 4, 319 – 331.
Romana-Eguia M, Ikedab M, Basiaoa Z, Taniguchib N.2004. Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA. Aquaculture236,131–150.
Saad Y, Rashed M, Atta A, Ahmed N. 2012. Genetic diversity among some Tilapia species based on ISSR markers. Life Science Journal(9),4841-4846.
Samaradivakara S, Hirimuthugoda N, Gunawardana R, Illeperuma R, Fernandopulle N, Silva A, Alexander P. 2012. Morphological Variation of Four Tilapia Populations in Selected Reservoirs in Sri Lanka. Tropical Agricultural Research 23,105-116
Sambrook L, Fritsch EF, Maniats T. 1989. Molecular Cloning: A laboratory manual ColdSpring Harbor Press. Cold Spring Harbor, N.Y.
Shair OHM, Al- Ssum RM, Bahkali AH. 2011. Genetic variation investigation of Tilapia grown under Saudi Arabian controlled environment. American Journal of Biochemistry and Molecular Biology(1),89-94.
Sofy HI, Layla AM, Iman MKA. 2008.Karyotypic diversity of some Tilapia species. Nature and Science (6),19-27
Soufy H, Laila AM, Iman MKA. 2009. RAPD-PCR for DNA-Fingerprinting of Egyptian Tilapia .New York Science Journal(2),20- 25.
Sun Z, Wang Z,Tu J, Zhang J,Yu F,McVettyP, Li G. 2007. An ultradense genetic recombination map for Brassica napus, consisting of 13551 SRAP markers.Theor. Applied Genetics 114,1305-1317.
Wang Z, Jayasankar p, Khoo S, Nakamura K, Sumantadinata K, Carman O, Okamoto N. 2000. AFLP Fingerprinting Reveals Genetic Variabilityin Common Carp Stocks from Indonesia. Asian Fisheries Science (13),139-147.
Zhang GRW, Ran W, Gardner JPA, Wei KJ, Wang WM. 2014a. Genetic Diversity of and Differentiation among Five Populations of Blunt Snout Bream (Megalobrama amblycephala) Revealed by SRAP Markers: Implications for Conservation and Management. PLoS ONE(9), e108967. http://dx.doi.org/10.1371/journal.pone.0108967
Zhang W, He H, Guan Y, Du H, Yuan L, Li Z, Yao D, Pan J, Cai R.2010. Identification and mapping of molecular markers linked to the tuberculate fruit gene in the cucumber (Cucumis sativus L.). Theoretical and Applied Genetics 120,645-654.
Zhang Y, Qin Y, Wang Z, Guo L, Chen X, Liang Z, Guo H. 2014b. DNA isolation and optimization of SRAP-PCR condition for endangered Polyporus umbellatus. Journal of Medicinal Plant Research, (8), 133-137.
Gomaah El Fadly, Ismael Khatab, Medhat Rehan, Alaa Kalboush (2016), Genetic Diversity in Egyptian Populations of Tilapia species Using RAPD and SRAP Markers; JBES, V8, N4, April, P231-243
https://innspub.net/genetic-diversity-in-egyptian-populations-of-tilapia-species-using-rapd-and-srap-markers/
Copyright © 2016
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0