J. Bio. Env. Sci.10( 5), 79-88, May 2017
The majority of Khyber Pakhtun Khwa (KPK) rice growing regions face problems of rapid floods each year which results soil erosion. Our current study aims to conserve the local varieties of rice growing in these floods alarming regions. About 40 genotypes were collected and evaluated through SDS-PAGE for genetic diversity. Sodium Dodicyle Sulphate Polyacrylamide Gel Electrophoresis protocol for seed storage protein profile of 40 genotypes were resolved adjusting the conditions on 12.25-15%. The experimental results from banding profile of 40 genotypes were evaluated statistically for cluster analysis and genetic disagreement. For more accuracy the electrophorogram of gels replicate were divided into three zones. The results from cluster analysis and genetic disagreement showed that present investigation was useful as provided information about 100% genetic relationships of genotypes, and with average low similarity coefficient and genetic variation of genotypes. These variations are due to the differences among various ecological conditions. However further collection and advance techniques are requires broadening the gene pool.
Ahmad M, Akhtar M, Anwar M. 2005. Basmati rice: progress, issues and prospects for Pakistan. Proceeding of the international seminar in rice crop. Rice research institute, Kala Shah Kaku. 1-7. http://www.aari.punjab.gov.pk
Buyukunak Bal EB, Bay S. 2010. Genetic analysis of Turkish rice varieties (Oryza sativa L.) using seed storage proteins and RAPD markers. Europion Food Research Technology 230, 609–617. https://link.springer.com
Cagampang GB, Cruz LJ, Espiritu SG, Santigu RG, Juliano BO. 1966. Studies on the extraction and composition of rice protein. Cereal Chemistry 43, 145. http://dx.doi.org/10.1094/CCHEM-09-14-0202-R
Ferguson JM, Grabe DF. 1986. Idetification of cultivars of perennial ryegrasss by SDS-PAGE of seed proteins. Crop Science 26, 170-176. https://dl.sciencesocieties.org
Ghafoor A. 1999. Genetic diversity of gene action in Vigna mungo based on morphological and biochemical markers. PHD theses. Quaid-i-Azam University Islamabad.
Ghafoor A, Ahmad Z, Qureshi AS, Bashir M. 2002. Genetic relationship in Vigna mungo (L.) Hepper and V. radiate (L.) R. Wilczek based on morphological traits and SDS-PAGE. Journal Euphytica 123, 367-378. http://dx.doi.org/10.1007/s10681-017-1895-3.
Grist DH. 1986. Rice. Trophical agricultural series. Longman Publisher, New York. https://www.abebooks.co.uk/9780582404038
Habib MSA, Wani GH, Habib M. 2000. Seed protein profile and isozyme polymorphism as markers for identification of some important rice cultivars. Applied Biological Research 2, 55-59. http://dx.doi.org/10.5958
Inamullah Khan IA, Ahmad H, Ghafoor S, Gul S, Muhammad I, Ilyas M. 2010. Seed Storage Protein Profile of Rice Varieties Commonly Grown in Pakistan. Asian Journal of Agricultural Sciences 2, 120-123. http://maxwellsci.com/jp/j2p.php?jid=AJAS
Iqbal MM, Goheer MA, Khan MA. 2009. Climate-change aspersions on food security of Pakistan. A scientific journal of Comsats – science vision 15, 15-23. pakistan/pamtr8b.htm) www.nssd.net/country
Iqbal SH, Ghafoor A, Ayub N. 2005. Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pakistan Jounal of Botany 37, 87-96. http://www.pakbs.org/pjbot/
Khan N, Ali N, Rabbani MA, Masood MS. (2010). Diversity of Glutelin Αlpha Subunits in Rice varieties from Pakistan. Pakistan Jounal of Botany 42, 2051-2057. www.pakbs.org/pjbot
Khan FS , Razzaq S, Irfan K, Maqbool F, Farid A, Illahi I, Amin TU. 2008. Dr. Wheat: A Web-based Expert System for Diagnosis of Diseases and Pests in Pakistani Wheat. Proceedings of the World Congress on Engineering Lodon UK.
Khush, G.S. 1997. Origin, dispersal, cultivation and variation of rice. Plant Molecular Biology 35, 25-34. https://link.springer.com/journal/11103
Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-5. http://dx.doi.org/:10.1038/227680a0
Nisar M, Ghafoor A, Khan MR, Asmatullah. 2009. First proteomic assay of Pakistan Pisum sativum germplasm relation to germplasm pattern. Russian journal of Genetics 45, 807-812. https://link.springer.com/journal/11177
Parker PG, Snow AA, Schug MD, Booton GC, Fuerst PA. 1998. What molecules can tell us about populations: choosing and using a molecular markers. Ecology 79, 361-382. http://dx.doi.org/10.2307/176939
Rabbani MA, Qureshi AA, Afzal M, Anwar R, Komatsu S. 2001. Characterization of Mustard [Brassica juncea (L.) CZERN. & COSS.] germplasm by SDS-PAGE of total seed proteins. Pakistan Journal of Botany 33, 173-179. http://www.pakbs.org/pjbot/
Salim MM, Ashfaq M. 2003. A Production Hand book, Balanced Fertilization for Maximizing economic crop yields. Pakistan Research Council, Islamabad; 1-8. www.parc.gov.pk/index.php/en
Sharief AE, Moursy SA, Salama AM, EL-Emery MI, Youssef FE. 2005. Morphological, molecular and biochemical identification of some rice (Oryza sativa L.) cultivars. Pakistan journal of biological science 5, 1275-1279. http://dx.doi.org/10.3923/pjbs.2017.124.131.
Siddiqui SU, Tumamaru T, Satoh H. (2010). Pakistan rice genetic resources–iii: SDS-PAGE diversity profile of glutelins (seed storage protein) Pakistan Journal of Botany 42, 2523-2530. www.pakbs.org/pjbot
Sultana T, Ghafoor A, Ashraf M. 2005. Genetic divergence in lentil germplasm for botanical descriptors in relation with geographic origin. Pakistan Journal of Botany 37, 61-69. www.pakbs.org/pjbot
Zahid MA, Akhtar M, Sabir M, Anwar M, Jamal A. 2005. Genotypic and phenotypic correlation and path analysis in coarse grain rice. Proceeding of the International Seminar in rice crop. Rice Research Institute, Kala shah kaku. www.aari.punjab.gov.pk