Welcome to International Network for Natural Sciences | INNSpub

Genetic diversity in some euphrates poplar (Populus euphratica O.) ecotypes in Iran using microsatellites (SSRs) markers

Research Paper | July 1, 2016

| Download 6

Hossein Tavakoli-Neko, Anoushirvan Shirvany, Mohammad Hassan Assareh, Mohammad Reza Naghavi,Mohammad Pessarakli

Key Words:

J. Bio. Env. Sci.9( 1), 434-440, July 2016


JBES 2016 [Generate Certificate]


Euphrates poplar (Populus euphratica Oliv.) is a unique woody species which is naturally distributed in desert areas of some parts of Asia and Africa. Because of its outstanding features, it is a model plant to study environment stress tolerance. This research was conducted to evaluate the genetic variation in 12 ecotypes of P. euphratica in Iran through 10 simple sequence repeats (SSRs) primers from 2015 to 2016. The average numbers of alleles observed in each ecotype was 6.43 and average numbers of effective alleles was 5.58. The average of observed heterozygosis was 0.65 and average of expected heterozygosis was 0.80. The ecotypes were complying with Hardy-Weinberg’s equilibrium in all loci, except Marand ecotype for two of the ten primers that showed deviation of the balance (p<0.05). The Shannon information index was 1.75. Analysis of molecular variance (AMOVA) showed that 3% of molecular variance belongs to intra-population and 97% belongs to inter-population. The PCA showed six principal components covered 22.86% of the total variance. Clustering analysis of ecotypes through genetic distance, the examined ecotypes were divided into six groups, while the geographic distance did not have any significant effect on genetic differences. Overall, the results indicated that P. euphratica stands covered a vast area of Iran in the past, and probably had not been fragmented; it seems vast areas of Iran are potentially ready for revival of P. euphratica forests.


Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Genetic diversity in some euphrates poplar (Populus euphratica O.) ecotypes in Iran using microsatellites (SSRs) markers

FAO. 1979. Poplar and Willow in wood production and land use 328 p.

Lin SZ, Zhang ZY, Zhang Q, Lin YZ. 2006. Progress in the study of molecular genetic improvements of poplar in China. Journal of Integrative Plant Biology 48(9), 1001-1007.

Murray MG, Thompson WF. 1980. Rapid isolation of high molecular weight plant. Nucleic Acids Research 8, 4321-4325.

Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceeding of the National Academy of Sciences of the USA 76, 5269-5273.

Pascal E, Steffen F, Martin S. 2009. Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica. Jour-nal of Forestry Research 20(3), 195-198.

Sabeti H. 2003. Forests, Trees and Shrubs of Iran. 3 edn. University of Yazd Yazd, Iran.

Saito Y, Shiraishi S, Tanimoto T, Yin L, Watanabe S, Ide Y. 2002 Genetic diversity of Populus euphratica populations in northwestern China determined by RAPD DNA. New Forests 23, 97-103.

Tuskan GA, DiFazio SP, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, et al. 2006. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray). Science 313(5793), 1596-1604.

Wang J, Li Z, Guo Q. 2011. Genetic variation within and among populations of a desert poplar (Populus euphratica) revealed by SSR markers. Annals of forest science 68, (1143-1149).

Wang J, Wu Y, Ren G, Guo Q, Liu J. 2011. Genetic Differentiation and Delimitation among Ecologically Diverged Populus euphratica and P.pruinosa. . PLoS ONE 6(10), e26530.

Wu Y, wamg W, Liu J. 2008. Development and characterization of microsatellite markers in Populus euphratica. Molecular Ecology Resources 8, 1142-1144.

Xu F, Feng, S, Wu, R, Du FK. 2013. Two highly validated SSR multiplexes (8-plex) for Euphrates’ poplar, Populus euphratica (Salicaceae). Molecular Ecology Resources 13, 144-153.