Int. J. Agron. Agri. Res.5(1), 7-15, July 2014
In Tunisia, Citrus sinensis culture is spread especially in Cap Bon region in the North East. It is represented by a large number of varieties. AFLP (Amplified Fragment Length Polymorphism) markers were used in order to study genetic diversity. Thirty accessions representing the majority of orange germplasm were collected from Cap Bon region. AFLP products were analyzed by capillary electrophoresis on an automated ABI Prism 3130 DNA sequencer. Using GeneMapper, AFLP bands were scored, across all genotypes, for presence (1) or absence (0) and transformed into 0/1 binary matrix. A total of 330 of polymorphic markers were revealed using 3 AFLP primer combinations. These markers expressed a high level of polymorphism allowing the distinction of all accessions. Resolving power (Rp) showed a high rate of collective Rp (97.75) with an average of 32.58. The Polymorphism Information Content (PIC) ranged from 0.16 to 0.22 with an average of 0.18 per primer pair. Genetic similarities were estimated basing on Nei and Li’s (1972) formula. The similarity coefficient between cultivars ranged from 0.15 to 0.96 with an average of 0.76. Most of the accessions showed a high degree of genetic similarity. Additionally, the relationship of the cultivars was also estimated using principal coordinate analysis (PCoA); the first three principal axes explained 94.56 of the total variation. Bioinformatic tools were very useful for investigating the genetic diversity of orange genotypes. Results of this study showed that AFLP markers can be useful tool for investigating the genetic diversity of orange genotypes.
Baraket G, Chatti K, Saddoud O, Mars M, Marrakchi M, Trifi M, Salhi Hannachi A. 2009. Genetic analysis of Tunisian fig (Ficus carica L.) cultivars using amplified fragment length polymorphism (AFLP) markers. Scientia Horticulturae 120, 487-492.
Barrett H C, Rhodes A M. 1976. A numerical taxonomy study affinity relationships in cultivated citrus and its close relatives. Systematic Botany 1, 105-136.
Davies F S, Albrigo L G. 1992. History, distribution and uses of citrus fruits. In: Citrus. CAB International, University Press, 1-11 Cambridge, UK.
DGPA. 2008. General Direction of Agricultural Production. Ministry of Agriculture and Hydraulic resources, Tunisia.
Falk DA, Holsinger KE. 1991. Genetics and conservation of rare plants. New York: Oxford University Press.
Gilbert JE, Lewis RV, Wilkinson MJ, Galigari PDS. 1999. Developing and appropriate strategy to assess genetic variability in plant germplasm collections. Theorical and Applied Gentics 98, 1125-1131.
Golein B, Talaie A, Zamani Z, Ebadi A, and Behjatina A. 2005. Assessment of genetic variability in some Iranian sweet oranges (Citrus sinensis [L.] Osbeck) and mandarins (Citrus reticulate Blanco) using SSR markers. International Journal of Agriculture and Biology 7, 167-170.
Jbir R, Hasnaoui N, Mars M, Marrakchi M, Trifi M. 2008. Characterization of Tunisian pomegranate (Punica granatum L.) cultivars using amplified fragment length polymorphism analysis. Scientia Horticulturae 115, 231-237.
JinPing X, LiGeng C, Ming X, HaiLin L, and WeiQi Y. 2009. Identification of AFLP fragments linked to seedlness in Ponkan mandarin (Citrus reticulate Blanco) and conversion to SCAR markers. Scientia Horticulturae 121, 505-510.
Kepiro JL, Roose ML. 2010. AFLP markers closely linked to a major gene essential for nucellar embryony (apomixis) in Citrus maxima × Poncirus trifoliate. Tree Genetics and Genomes 6, 1–11.
Krichen L, Bourguiba H, Audergon J M, Trifi-Farah N. 2010. Comparative analysis of genetic diversity in Tunisian apricot germplasm using AFLP and SSR markers. Scientia Horticulturae 127, 54-63.
Liu CM, McElever J, Tzafrir I, Joosen R, Wittich P, Patton DV, An Lammeren AAM, Meinke D W. 2002. Condensin and cohesion knockouts in Arabidopsis exhibit a titan seed phenotype. The Plant Journal 4, 405-415.
Lynch M., Walsh J.B. 1998. Genetics and analysis of quantitative traits. Sinauer Assocs: Inc. Sunderland, MA.
Meudt HM, Clarke AC. 2007. Almost forgotten or latest practice? AFLP applications, analyses, advances. Trends in Ecololgy and Evolution 12, 106-117.
Moslemi M, Zahravi M, Bakhshi Khaniki G. 2010. Genetic diversity and population genetic structure of pomegranate (Punica granatum L.) in Iran using AFLP markers. Scientia Horticulturae 126, 441-447.
Mueller UG, Wolfenbarger LL. 1999. AFLP genotyping and fingerprinting. Trends in Ecololgy and Evolution 14, 389-394.
Mzali MT, Lasram M. 2007. L’arboriculture fruitière en Tunisie, Vol3: Les arbres à pépins, les agrumes et la vigne de table.
Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences 76, 5269-5273.
Nicolosi E, Deng Z N, Gentile A, La Malfa S, Continella G, Tribulato E. 2000. Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theorical and Applied Gentics 100, 1155-1166.
Prevost A, Wilkinson M J. 1999. A new system of comparing PVR primers applied to ISSR fingerprinting of potato cultivars. Theorical and Applied Gentics 98, 107-112.
Rohlf FJ. 1989. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System version 2.02, Exeter software. New York: Setauket
Saghai-Maroof NA, Soliman KM, Jorgensen RA, Allard R. 1984. Ribososmal RNA spacer-length polymorphism in barley: mendelian inheritance, chromosomal location and population dynamics. Proceedings of the National Academy of Sciences 81, 8014-8018.
Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2nd ed. NewYork: San Francisco, Cold Spring Harbor laboratory, Cold Spring Harbor.
Sensi E, Vignani R, Scali M, Masi E, Cresti M. 2003. DNA fingerprinting and genetic relatedness among cultivated varieties of Olea europea L. estimated by AFLP analysis. Scientia Horticulturae 97, 379-388.
Sokal RR, Michener CD. 1958. A statistical method for evaluating systematic relationships. The University of Kansas science bulletin 38, 1409-1438.
Song N, Zhang X M, Gao TX. 2010. Genetic diversity and population structure of spotted tail goby (Synechogobius ommaturus) based on AFLP analysis. Biochemical Systematics and Ecology 38, 1089-1095.
Swingle WT, Reece PC. 1967. The botany of citrus and its wild relatives; In: The Citrus industry, Vol 1, ed. W Reuther H J Webber L D Batchelor, 389-390 USA, University of California Press, Berkley, CA.
Talon M., Gmitter FGJ. 2008. Citrus genomics. International Journal of Plant Genomics 1-17.
Vos P, Hoger R, Bleeker M, Rejans M, Vandelee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407-4417.