Genetic diversity of Tribolium castaneum (Herbst) population in storage infrastructures and agro ecological zones in Senegal

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Research Paper 01/09/2013
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Genetic diversity of Tribolium castaneum (Herbst) population in storage infrastructures and agro ecological zones in Senegal

Toffène Diome, Cheikh Thiaw, Malick Sarr, Assane Ndong, Mamadou Kane, NDiaga Cissé, Mbacké Sembène
Int. J. Biosci. 3(9), 248-258, September 2013.
Copyright Statement: Copyright 2013; The Author(s).
License: CC BY-NC 4.0

Abstract

The stocks of millet are attacked by the beetle among them T. castaneum. This devastator attacks the grain and causes important losses as for as the stocks of ground millet in the sahel. The aim of this study is to evaluate genetic diversity of T. castaneum population meet in the stocking infrastructures and the different agroecological areas with the intention to identify the different haplotypes that walk around. Portions of the cytochrome b and 28S ribosomal genes of T. castaneum were sequenced, using samples from that represent storage infrastructures and agroecological areas in Senegal. The results reveal a strong haplotype diversity in the stores of Sandiara (0.889±0.127) and Diaroume (0.556±0.165). The population of T. castaneum meet in the stores who supply themselves locally as that of Djilas show a weak haplotypique and nucleotidic diversity. The cereals marketing at the markets such as the Sandiara one would be at the origine of the wealth haplotypic observed in the store taken as an exemple in this locality. The pourcentage (0.16%) of molecular variance analysis (AMOVA) shows that there is not a significant genetic differentiation between agroecological areas. The test of Mantel shows that there is no correlation between geographical distance and genetic differenciation.

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