Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | February 1, 2016

VIEWS 1
| Download 1

Genetic polymorphism in Lens culinaris collected from malakand division Khayber Pakhtunkhwa, Pakistan

Syed Fazal Wadood, Nazim Hassan, Anela Khaliq, Nausheen,Tour jan, Abdul Ghafoor, Murad Khan, Mohammad Nisar

Key Words:


J. Bio. Env. Sci.8(2), 53-60, February 2016

Certification:

JBES 2016 [Generate Certificate]

Abstract

A major part of the human di et al over the world consists of cereals and legumes. Legumes are however, considered as the major source of protein and dietary amino acid for man and farm animals. The detailed evolutionary relations and cluster analysis in this group of plants may help in further manipulations and modifications of plants leading to enhanced nutritional supplementation for consumption. To estimatethe picture of genetic diversity in lentil (Lens culinaris)the proteomics assay was carried out. A total of 60 cultivars were collected from 60 lentil growing areas of Malakand Division Khyber Pakhtunkhwa, Pakistan. The obtained lines were biochemically evaluated for the purpose to check the genetic heterogeneity. Based on proteomics homology dendrogram and cluster plottingsorted 60 cultivars into 4 clusters i.e. Cluster-1 (sorted forty cultivars), cluster-2 (grouped single cultivar), cluster-3 (had seventeen genotypes) and cluster-4 (grouped two cultivars).Thirteen loci (bands) were spotted in the collected germplasm of lentils through SDS-PAGE. Intra species locus contribution toward genetic diversity (LCTGD) was 84.61. Out of thirteen loci, locus-1, 2, 3,4,5, 6,7,8, 9, 10 and 11 showed polymorphic.The results showed that SDS-PAGE displayed a low level Genetic polymorphism in our accessions but higher than in other legumes, and it can be increased by the addition of more extensively sourced germplasm.

VIEWS 1

Copyright © 2016
By Authors and International Network for
Natural Sciences (INNSPUB)
http://innspub.net
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Genetic polymorphism in Lens culinaris collected from malakand division Khayber Pakhtunkhwa, Pakistan

Akhtar M. 2001. Phylogenetic relationship amongVigna species based on agronomic and biochemical analysis. M Phil Thesis, Quaid-I-Azam University, Pakistan. 99.

Amar AA, Zohra FLF, Noureddin Y. 2014. Genetic diversity of seed storage protein in Medicagotruncatula genotypes in relation with salt stress tolerance.International journal of agriculture and science 7-2, 55-69.

Anonymous. 2003. Lentil situation and outlook Bi-weekly Bulletin.13 (21). http://www.agr.ca/policy/winn/biweekly/index.htm

Bhatty RS. 1998. Composition and quality of lentil (Lens culinaris Medik).A review. Canadian Institute of Food Science and Technology 21, 144–160.

Bretting PK, Widrlechner MP. 1995. Genetic Markers and Plant Genetic Resources, Plant Breed 13, 11–86.

Cokkizgin A, Shtaya MJY. 2013. Lentil: Origin, Cultivation Techniques, Utilization and advance in transformation. Science and Education Centre of North America 1, 55-62.

Cubero JI. 1981. Origin, taxonomy and domestication. In: Webb C, Hawtin G [eds.], Lentil, 15–38. C.A.B., Landon, UK.

Duke JA. 1981. Hand bookof legumes of world economic importance, Plenum Press, New York. 52– 57.

Duran Y, Vega MP. 2004. Assessment of genetic variation and species relationship in a collection of Lens using RAPD and ISSR markers.

Ferreira JJ, Lvarrez EA, Roca MA, Giralde R. 2000. Determination of the out crossing rate of Phaseolus vulgaris L. using seed protein markers.Euphytica 113, 259-263.

Fikiru E, Tesfaye K, Bekele E. 2007. Genetic diversity and population structure of Ethiopian lentil (Lens culinaris Medikus) landraces as revealed by ISSR marker. African Journal of Biotechnology 6(12), 1460-1468.

Ford RR, Taylor PWJ. 2003. Construction of an intraspecific linkage map of lentil (Lens culinaris ssp. culinaris). Theor.Applied Genetics 107, 910–916.

Ganjali S, Siahsar BA, Allahdou M. 2012. Investigation of genetic variation of lentillines using random amplified polymorphic DNA (RAPD) and intron-exon splice junctions (ISJ) analysis. International Research Journalof Applied and Basic Science 3, 466–478.

Ghafoor A, Gulbaaz FN, Afzal M, Ashraf M, Arshad M. 2003. Inter-relationship between SDS-PAGE markers and agronomic traits in chickpea (CicerarietinumL.). Pakistan Journal of Botany 35(4), 613-624.

Ghafoor A. Ahmad Z, Qureshi AS, Bashir M. 2002. Genetic relationship in Vignamungo(L.) Hepper and V. radiata(L.) R. Wileczek based on morphological traits and SDS-PAGE. Euphytica 123, 378.

Hameed A, Shah TM, Atta BM, Iqbal N, Haq MA, Ali H. 2009. Comparative Seed Storage Protein Profiling Of Kabuli Chickpea Genotypes. Pakistan Journal of Botany 41(2), 703-710.

Iqbal SH, Ghafoor A, Ayub N. 2005. Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pakistan Journal of Botany 37, 87-96.

Lioi L, Sparvoli F, Bollini R. 1999. Variation and genomic polymorphism of lectin-related protein in Lima Bean (PhaseoluslunatusL.) seed.Genetic Resources and Crops Evaluation 46, 157-182.

Nisar M, Ghafoor A, Asmatullah. 2009. First Proteomic Assay of Pakistani PisumsativumL. Germplasm Relation to Geographic Pattern, Russian Journal of Genetics 45(7), 805–810.

Nisar M, Ghafoor A, Khan MR, Ahmad H, Qureshi AS, Ali M. 2007. Genetic diversity and geographic relationship among local and exotic chickpea germplasm.Pakistan Journalof Bot. 39(5), 1575-1581.

Picard P, Greneche MB, Zivy M. 2005.Two-Dimensional Electrophoresis, potential of two-dimensional electrophoresis in routine identification of closely related durum wheat lines.Electrophoresis 18, 174–181.

Piergiovanni AR, Taranto G. 2003. Geographic distribution of genetic variation in a lentil collection by SDS-PAGE fractionation of seed storage proteins. Journal of Genetics and plant Breeding 57, 39-46.

Przybylska   J,    Zimniak-Przybylska    Z.   1995. Electrophoretic pattern of seed globulins in the Old World Lupinusspecies.Genetic Resources and Crop Evolution 42, 69–75.

Rabbani M, AAQureshi A, Afzal M, Anwar R, Komatsu S. 2001. Characterization of mustard Brassica junncea (L.) Cezern.& Cross germplasm by SDS-PAGE of total seed protein. Pakistan Journal of Botany 33(3), 173-179.

Sultan S, Ghafoor A, Ashraf M. 2006. Geographic pattern of Diversity of cultivated lenti germplasm collected from Pakistan as assessed by seed protein assays.ActaBiologicacracoviensia series Botanica 48(1), 77–84.

Thiellement H, Bahraman N, Damerval C, Plomion C, Rossignal M, Santoni V, DeVienne D, Zivy M. 1999. Proteomics for genetic and physiological studies in plants.Electrophoresis 20, 2013–2026.

SUBMIT MANUSCRIPT

Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background