home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Genetic polymorphism within the wild population of Rhynchosia himalensis Benth. ex Baker

Paper Details

Research Paper 01/03/2019
Views (431) Download (19)
current_issue_feature_image
publication_file

Genetic polymorphism within the wild population of Rhynchosia himalensis Benth. ex Baker

Muhammad Khalil Ullah Khan, Noor Muhammad, Nisar Uddin, Niaz Ali, Ikram Khan, Rahim Ullah
Int. J. Biosci.14( 3), 54-64, March 2019.
Certificate: IJB 2019 [Generate Certificate]
Key: Genetic diversityMorphologyR. himalensisSDS-PAGE

Abstract

The importance of plant genetic diversity is now being recognized as a specific area since exploding population with urbanization and decreasing cultivable lands are the critical factors contributing to food insecurity in developing world and are also helpful in conservation planning strategies of threatened plant species. For this purpose thirty genotypes of Rhynchosia himalensis were collected from the different areas of Swat and were assessed for morphometric and for SDS-PAGE and this study revealed that genetic assortment were present at both morphological and molecular levels. The intraspecific variations were present among the genotypes. The examined morphological data (which includes quantitative and qualitative data) exposed that variation was present among the genotypes of above specie. Likewise it was found that two alleles were responsible in controlling leaf color, leaf upper surface with Emerald green 70% and leaf lower surface with 30% yellow green. Other leaf color was 70% Moss green and 30% white respectively. 100% seeds were flat shape. The seed color was found to be brown for all of the genotypes, Hillum color was found to be yellow. Seed texture was of two types Rough and Smooth. The rough was 60% and Smooth was of 40%.The seed storage protein profiles of twenty R. himalensis genotypes   were studied for SDS-PAGE and total seed storage proteins were best resoluted on 12% polyacrylamide Gel. A total of 9 reproducible bands with molecular weight ranging from 10 to 180kDa were detected. The genotypes were distributed into two Regions R-I, R-II and R-III. Based on the intraspecific locus variation among 30 genotypes of R. himalensis L-3, L-6 and L-8 were monomorphic and the locus contribution toward genetic disagreement (LCTGD) of Rhynchosia himalensis was 33.333%. Further investigation is required for genetic diversity in the R. himalensis.

VIEWS 18
Cover PDF

Achuba FI. 2006. The effect of sub letethal concentration of crude oil on the growth and metabolism of cowpea (Vigna unguiculata) seedlings. Environmentalist 21(1), 17 – 20.

Albert PS, Gao Z, Danilova TV, Birchler JA. 2010.  Diversity of Chromosomal Karyotypes in Maize and its Relatives. Cytogenet Genome Research 129 (1-3), 6-16.

ALI HH. 2013. Germination ecology of Rhynchosia capitata: an emerging summer weed in Asia. Planta Daninha, 31(2), p 249-257.

Ayten C, Leyla A, Zeki A. 2009. Biosystematics studies among Ebenus L. species based on morphological, RAPD-PCR and seed protein analysis in Turkey. Pakistan Journal of Botany 41(5), 2477-2486.

Beckstrom-Sternberg SM. 1989. Two-dimensional gel electrophoresis as a taxonomic tool: evidence from the Centrspermae. Biochemical and Systematic Ecology 17, 573–582.

Celis JE, Bravo R. 1984. Two dimensional Gel Electrophoresis of Proteins: Methods and applications. Academic Press New York.

Chen F, Liu H, Yao Q, Fang P, Lv F. 2015. Genetic variations and evolutionary relationships among radishes (Raphanus sativus L.) with different flesh colors based on red pigment content, karyotype and simple sequence repeat analysis. African J of Biotech 16(50), 3270-3281.

Cholastova T, Knotova D. 2012.Using Morphological and Microsatellite (SSR) Markers to Assess the Genetic Diversity in Alfalfa (Medicago sativa L.).International Journal of Biological Sciences 6(9), 781-787.

Das AB, Mohanty IC, Mahapatra D, Mohanty S, Ray A. 2010.Genetic variation of Indian potato (Solanum tuberosum L.) genotypes using chromosomal and RAPD markers. Crop Breeding and Applied Biotech 10(3), 238-246.

DOGRA KS. 2009. Comparison of understory vegetation in exotic and indigenous tree plantations in Shivalik Hills of N.W. Indian Himalayas (Himachal Pradesh). Journal of Ecology and the Natural Environment 1(5), p 130-136.

Egbadzor K, Ofori K, Yeboah M, Aboagye L, Opoku-Agyeman M, Danquah E, Offei S. 2014. Diversity in 113 cowpea [Vigna unguiculata (L) Walp] accessions assessed with 458 SNP markers. Springer Plus 3, 1–15.

Ghafoor A, Arshad M. 2008. Seed protein profiling of Pisum sativum L., germplasm using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for investigation of biodiversity. Pakistan Journal of Botany 40(6), 2315-2321.

Ghafoor A, Gulbaaz FN, Afzal M, Ashraf M,  Arshad M. 2003. Inter-relationship between SDS-PAGE markers and agronomic traits in chickpea (Cicer arietinum L.). Pakistan Journal of Botany 35(4), 613-624.

Hameed A, Shah TM, Atta BM, Iqbal N, Haq MA,  Ali H. 2009. Comparative Seed Storage Protein Profiling of Kabuli Chickpea Genotypes. Pakistan Journal of Botany 41(2), 703-710.

Hussein H, George NM. 2002. Taxonomic importance of floral morphology, chromosome number and seed protein electrophoretic patterns in some species of tribe Vicieae (subfamily: Papilionoideae – Leguminosae). Egy. J. Biotechnol. 11, 106-123.

Hussein H, George NM, El-Dimerdash MM. 2005. Taxonomic importance of seed protein electrophoretic patterns in some taxa of the subfamily Mimosoideae-Leguminosae. Assiut University Journal of Botany 34(2), 101-130.

Xu Y. 2009. Molecular Plant Breeding. CABI, South Asia. ILDIS: online. International Legume Database and Information Service. [cited 2010 January11]. Available from: http://www.ildis.org/LegumeWeb,2010.

Iqbal SH, Ghafoor A, Ayub N. 2005. Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pakistan Journal of Botany 37, 87-96.

Javid A, Ghafoor A, Anwar R. 2004. Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pakistan Journal of Botany 36, 87-96.

Kaga A, TomookaN, Hosaka K, Kamijima O. 1996. Species relationship in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis. Euphytcia 88, 17-24.

Ladizinsky G, Hymowitz T. 1979. Seed protein electrophoresis in taxonomic and evalotionary studies. Theoretical and Applied Genetics 54, 145-151.

Lioi, Sparvoli F, Bollini R. 1999. Variation and genomic polymorphism of lectin-related protein in Lima Bean (Phaseolus lunatus L.) seed. Genetic Resources and Crops Evaluation 46, 157-182.

Manifesto MM, Schlatter AR, Hopp HE. 2001. Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Science 41(3), 682–690

Muhammad N, Ali  N, Nisar  M, Abd Allah  EF, Hashem  A, Alqarawi A, Aldubise  A,  Khan U, Rahman IU, Afza R, Khan A,  Ahmad  H. 2018. Genetic Diversity within Natural Populations Of The Medicinal Plant Rhynchosia minima (L.) Dc. Applied Ecology And Environmental Research 16(5), 5633-5651.

Muhammad N, Ali N, Uddin N, Wadood SF, Khan KU, Alam N, Haq I. 2018a. Evaluation of informants consensus factor of medicinal uses of bryophytes in Swegalai Valley KPK, Pakistan. Journal of Biodiversity and Environmental Sciences 12(5), p 57-63.

Muhammad N, Wadood SF, Khan W, Ali N, Nisar M. 2018b. Intra-species profiling of Cleome viscosa growing in Swat district (Pakistan). Biosystems Diversity 26(1), 52–55. http://dx.doi.org/10.15421/011808

Ndiaye M, Faye B, Tine R. 2012. Assessment of the Molecular Marker of Plasmodium falciparum Chloroquine Resistance (Pfcrt) in Senegal after Several Years of Chloroquine Withdrawal. The American Journal of Tropical Medicine and Hygiene 87, 640–645

Ndoutoume-Ndong A, Rojas-Rousse D. 2007. Y’at’il elimination d’ Eupelmus orientalis Crawford par Eupelmmusvuilleti Crawford (Hymenoptera: Eupelmidae) dessystemes de stockages du niebe (Vigna unguculata Walp). Annales de la Sociétéentomologique de France 43, 139-144.

NG, NO, Monti LM. 1990. Cowpea genetic resources. International Institute of Tropical Agriculture IITA. Amarin Printing Group Co. Ltd. 200.

Oppong-Konadu EYR, Akromah HK, Adu-Dapaah OE. 2005. Genetic diversity within Ghanaian cowpea germplasm based on SDS – PAGE. African Crop Science Journal 13(2), 117–123.

Pal T, Ghosh S, Mondal A, De KK. 2016. Evaluation of genetic diversity in some promising varieties of lentil using karyological characters and protein profiling. Journal of Genetic Engineering and Biotechnology – In Press 14(1), 39-48.

Pasquet RS, Baudoin JP. 2001. Cowpea. In: Charrier A. Jacquot M., Hamon S. and Nicolas D. (eds), Tropical Plant, Breeding. Science publishers, Enfield, p 177–198.

Potokina E, Duncan A, Vaughan A, Eggi EE, Tomooka N. 2000. Population diversity of the Vicia sativa agg. (Fabaceae) in the flora of the former USSR deduced from RAPD and seed protein analysis. Genetic Resources and Crop Evolution 47, 171-183.

Sammour RH. 1991. Using electrophoretic techniques in varietal identification, biosystematic analysis, phylogentic relations and genetic resources management. Journal of Islamic Academy of Sciences 4, 221–26.

Sharma NK, Sharma MM, Sen DN. 1978. Seed perpetuation in Rhynchosia capitata DC. Biologia Plantarum 20, 225-228.

Simon L, Shyamalamma S, Narayanaswamy P. 2007. Morphological and molecular analysis of genetic diversity in Jackfruit. Journal of Horticultural Science & Biotechnology 82(5), 764-768.

Singh BB, Ajeigbe HA, Tarawali SA, Fernandez-Rivera S, Abubakar M. 2003. Improving the production and utilization of cowpea as food and fodder. Field Crops Research 84, 169–177.

Steele WM. 1972. Cowpea in Africa. Doctoral thesis. University of Reading, United Kingdom. studies. Theoretical and Applied Genetics 114, 1105–1116.

Sundin BA, Chun-Hwei Chiu, Riffle M, Davis TN, Eric G, Muller D. 2004.  Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21(9), 793-800.

Varshney RK, Beier U, Khlestkina E, Kota R, Korzun V, Röder M, Graner A, Börner A.  2007. Single nucleotide polymorphisms in rye: discovery, frequency and applications for genome mapping and diversity studies. Theoretical and Applied Genetics 114, 1105-1116.

Win KT, Zaw A, New KL, Thein MS, Yutaka H. 2011. Diversity of Myanmar cowpea accessions through seed storage polypeptides and its cross compatibility with the subgenus Ceratotropis. Journal of Plant Breeding and Crop Sciences 3(5), 87-95.

Submit Manuscript