Genetic relationships to five species of Acacia (Fabaceae) in Iraq using PCR-Rapd Technique

Paper Details

Research Paper 01/03/2019
Views (607)
current_issue_feature_image
publication_file

Genetic relationships to five species of Acacia (Fabaceae) in Iraq using PCR-Rapd Technique

Khansaa Rasheed Al-Joboury
Int. J. Biosci. 14(3), 273-277, March 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

The aim of this study is to assess the genetic relations among species and to know the extent of convergence and divergence between them. The phylogentic relationships for five genotypes ofAcacia analyzed with RAPD-PCR (Random Amplified Polymorphic DNA ) technique, The DNA for fresh and dry young leaves isolated from each sample for RAPD method, ten primers was studiedAnd used to identify the species of converged and similar in their characteristics or species diverged due to the difference of most of their qualities. The species were Acacia nilotica,  Acacia seyal, Acacia tortilis, Acacia farnesiana, Acacia reniformis, And the primers which used in this study were:A01: CCCAATTGG, A02: AACGGCTTC, C03: TCCCGAAGC , C08: AACGCTGTC , D05: GAACTTCGC, OPA-1: TTGAACCCG , OpA-2: ACAGTTGAG,OpA-3: TAGGCGTAG ,OpA-4: TATGTCGGC, OpA-5: ATAGTAGCC,  were tested in this study. In this study The band size ranged from 150 ‐ 1500 bp, So the total band ranged from 18-38 and the polymorphic bands ranged from 9 in the primer OpA-2 to 22 in the primer  OPA-1.We suggest that chemical, geographic and genetic studies support and support the morphological studies of plant species and help us to differentiate among plant species.

Boer E. 2002. Acacia senegal (L.) Willd. Record from Protabase. In: Oyen, LPA, Lemmens RHMJ, (eds) PROTA (Plant Resources of Tropical Africa), Wageningen, the Netherlands.

Casiva V, Saidman O, Vilardi E, Cialdella  M. 2002. First Comparative phonetic studies of Argentinean species of Acacia (Fabaceae), using morphometric, isozymal, and RAPD approach. American Journal of Botany 89, 843-845.

Ding G, Ding X, Shen J, Tang F. 2005. Genetic diversity and molecular authentication of wild populations of Dendrobiumofficinale by RAPD. ActaP harmaceutica Sinica 40, 1028-1032.

Gherardi M, Mangin B, Goffinet B, Bonnet D, Huguet T. 1998. A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular marker. Theoretical and Applied Genetics 98, 406-412.

Haerinasab M, Rahiminejad M. 2012 A taxonomic revision of the genus TrifoliumL. sect. Fragifera Koch (Fabaceae) in Iran. Iranian journal of botany 18, 22-30.

Hamza N, Habeballa R, Abdalla E. 2009. Phylogenetic relationship within indigenous Sudanese Cassia senna (L), using RAPD molecular markers. African Journal of Biotechnology 8, 4824-4829.

Khadidiatou N, Samb P, Chevallier M. 2008. Genetic Variability Analysis of the Polyploid Complex of Acacia nilotica (L.) Willd using RAPD Markers. Tropicultura  26, 135-140.

Gherardi M, Mangin B, Goffinet B, Bonnet D, Huguet T. 1998. A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular marker. Theoretical and Applied Genetics 98, 406-412.

Masi  L, Cipollaro  M, Bernardo G, Galderisi U, Galano G, Cascino A, Grassi G, Pavone E, Simeone A. 2003. Clonal selection and molecular characterization by RAPD analysis of the fig Ficuscarica L. Dottato’’ and ‘‘Blanco del Cilento’’ cultivars in Italy. Acta Horticulture 605, 65-68.

Miller T, Bayer R. 2001.Molecular phylogenetics of Acacia (Fabaceae: Mimosoideae) based on chloroplast matK coding sequence and flanking trnK intron spacer regions. American Journal of Botany 88, 697-705.

Niknejad A, Kadir M, Kadzimin S, Abdulla N. Sorkheh K. 2009. Molecular characteristics and phylogenetic relationships among and within species of Phalaenopsis (Epidendroideaeorchidaceae) based on RAPD analysis. African Journal of Biotechnology 8, 5225-5240.

Özbek F, Ufuk M, Ekici M. 2014. Morphological, anatomica, pollen and seed morphological properties of Melilotus bicolor Boiss. &Balansa (Fabaceae) endemic to Turkey. Australian Journal of Crop Science 8, 543-549.

Rami H, Eisa E. 2008. Phylogenic analysis in Acacia senegal using AFLP molecular markers across the Gum Arabic Belt in Sudan. African Journal of Biotechnology 7, 2333-2340.

Rashmi M, Nanda S, Rout C. 2004. Studies on genetic relatedness of Acacia tree species using RAPD markers, Biologia, Bratislava 59, 115-120.

Souza S, Carpentieri-Pípolo V, Ruas F, Carvalho V. 2008. Comparative analysis of genetic. Diversity among the maize inbred lines (Zea mays L.) obtained by studying genetic relationships in Lactuca spp. Theoretical and Applied Genetics 93, 1202–1210.

Verbeken D, Dierckx S, Dewettinck K. 2003. “Exudate gums: occurence, production, and applications” Appl. Microbiol. Biotechnol 63, 10-21.

Wang L, Grusak M. 2005. Structure and development of Medicago truncatula pod wall and seed coat. Annals of Botany 95, 737–747.

Related Articles

Yield performance assessment of different mustard cultivars under field conditions

Md. Khan Jahan Ali, Md. Moshiur Rahman, Kamrun Nahar, Sharmin Ara Jannat, Mst. Khadija Khatun, Sushan Chowhan, Md. Habibur Rahman, Int. J. Biosci. 27(2), 264-269, August 2025.

Species composition of xylomicobiota of some woody plants distributed in Azerbaijan

K. F. Bakhshaliyeva, V. Y. Hasanova, N. R. Namazov, B. N. Aliyeva, S. C. Garayeva, S. E. Nagiyeva, P. Z. Muradov, Int. J. Biosci. 27(2), 254-263, August 2025.

Perception of producers and processors on sorghum diversity in the context of climate change in center and Northern Benin

Parfait Segla Alohoutade1,2,3, Alphonse Sako Avocefohoun*1,2,3, Sènan Vodouhe2, Mohamed Kanazoe1,2,3, Nicodème Chabi1, Lamine Said Baba-Moussa3, Int. J. Biosci. 27(2), 243-253, August 2025.

Epizootic status and eradication of parasitic diseases in brown bears transferred to the rehabilitation center in Azerbaijan

Siala İ. Rustamova, Aygun A. Azizova, Gular R. Mammadova, Ramin S. Mammadov, Int. J. Biosci. 27(2), 236-242, August 2025.

Cytotoxic and apoptotic effects of Annona squamosa (Atis) crude leaf extract against A549 (Human Lung Adenocarcinoma) cell line

Ashton U. Lim, Noricel U. Garcia, Alkauzar H. Tantong, Int. J. Biosci. 27(2), 226-235, August 2025.

Perceptions of stakeholders towards sheep and goat dairy products in Benin

Yvette Adje, Philippe Sessou, Aretas Tonouhewa, Paulin Azokpota, Lamine Baba-Moussa, Souaïbou Farougou, Int. J. Biosci. 27(2), 211-225, August 2025.

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.

Agronomic performance and profitability of coffee wildlings using different soil media mixtures

Maribel L. Fernandez, Ricardo B. Casauay, Ronel A. Collado, Int. J. Biosci. 27(2), 189-199, August 2025.