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Genetic variation in critically endangered plant Amsonia orientalis Decne

Özlem Aksoy, Tuba Erbulucu, Fazıl Özen, Asuman Deveci

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J. Bio. Env. Sci.3(1), 44-53, January 2013

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Abstract

Amsonia orientalis Decne. (Apocynaceae) is a medical plant which has very restricted distribution only in west of Turkey and east of Greece on the world. Because of containing various alkaloids this species has anticancerogenic and antitumour effects. RAPD-PCR and SDS-PAGE were used to investigate the pattern of genetic variation among four known natural populations of A. orientalis. Polymorphic bands were obtained with 34 primers and pair wise genetic distance between samples was calculated using POPGENE package version 1.32. Genetic distances were calculated for all the species studied at both RAPD-PCR and SDS-PAGE methods. According to RAPD-PCR results the lowest genetic identity value (0.2881) was found between populations Ömerli and Gaziosmanpaşa, while the highest genetic distance value (1.2443) was found between populations Gaziosmanpaşa and Ömerli. The dendogram which is obtained from the SDS-PAGE analyses, allows two main groups to be distinguished. The first group contains Ömerli population and the second group has Gaziosmanpaşa, Paşaalanı and Adnanmenderes populations. The observed genetic variations showed that Ömerli and one of Balıkesir population should be considered in any planned in situ or ex situ conservation programs for this critically endangered plant species.

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Genetic variation in critically endangered plant Amsonia orientalis Decne

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