Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | January 1, 2013

| Download

Genetic variation in critically endangered plant Amsonia orientalis Decne

Özlem Aksoy, Tuba Erbulucu, Fazıl Özen, Asuman Deveci

Key Words:

J. Bio. Env. Sci.3(1), 44-53, January 2013


JBES 2013 [Generate Certificate]


Amsonia orientalis Decne. (Apocynaceae) is a medical plant which has very restricted distribution only in west of Turkey and east of Greece on the world. Because of containing various alkaloids this species has anticancerogenic and antitumour effects. RAPD-PCR and SDS-PAGE were used to investigate the pattern of genetic variation among four known natural populations of A. orientalis. Polymorphic bands were obtained with 34 primers and pair wise genetic distance between samples was calculated using POPGENE package version 1.32. Genetic distances were calculated for all the species studied at both RAPD-PCR and SDS-PAGE methods. According to RAPD-PCR results the lowest genetic identity value (0.2881) was found between populations Ömerli and Gaziosmanpaşa, while the highest genetic distance value (1.2443) was found between populations Gaziosmanpaşa and Ömerli. The dendogram which is obtained from the SDS-PAGE analyses, allows two main groups to be distinguished. The first group contains Ömerli population and the second group has Gaziosmanpaşa, Paşaalanı and Adnanmenderes populations. The observed genetic variations showed that Ömerli and one of Balıkesir population should be considered in any planned in situ or ex situ conservation programs for this critically endangered plant species.


Copyright © 2013
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Genetic variation in critically endangered plant Amsonia orientalis Decne

Akyalçın H, Özen F, Dulger B. 2006. Anatomy, morphology, palynology and antimicrobial activity of Amsonia orientalis Decne. (Apocynaceae) growing in Turkey. International Journal of Botany 2(1), 93-99.

Artyukova  EV,  Kozyreko  MM,  Koren  OG. 2004. RAPD and allozyme analysis of genetic diversity in Panax ginseng C.A. Meyer and P. quinquefolius L. Russian Journal of Genetics 40 (2), 239-247.

Babaoğlu S, Açık L, Çelebi A, Adıgüzel N. 2004. Molecular analysis of Turkish Alyssum L. (Brassicaceae) species by rapd-pcr and sds-page methods. Gazi Universitesi Journal Of Science 17 (3), 25-33.

Bardakçı F. 2001. Random amplified polymorphic DNA (RAPD) markers. Turkish Journal of Biology 25, 185-196.

Barrett SCH, Kohn JR. 1991. Genetics and evolutionary consequences of small population size in plants: implications for conservation in: Falk, D.A., Holsinger, K. E. (Eds.) Genetics and conservation of rare plants. Oxford University Press, New York, 55-60.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantites of protein utilizing the prencipal of protein-dye binding. Annual Biochemistry 72, 248-254.

Carter MC, Sytsma MD. 2001. Comparison of the genetic structure of North and South American populations of a clonal aquatic plant. Biological Invasions (3), 113–118.

Collins D, Mill RR, Möller M. 2003. Species separation of Taxus baccata, T. canadensis, and T. cuspidata (Taxaceae) and origins of their reputed hybrids inferred from RAPD and cpDNA data. American Journal of Botany 90, 175–182.

Cruse-Sanders JM, Hamrick JL. 2004. Genetic diversity in harvested and protected populations of wild American ginseng, Panax quinquefolius L. (Araliaceae). American Journal of Botany 91, 540– 548.

Dabine Lengyel E, Turiak G, Nyaradiné-Szabady J. 1986. Determination of secologanin content from shoots of Rhazya orientalis (Decne) A. DC. using the HPLC method. Herba Hungarica 25 (2), 141–150.

Davis PH. 1978. Flora of Turkey and East Aegean Islands. Univ. Press of Edinburg 6, 161.

Ekim T, Koyuncu M, Vural M. 2000. Türkiye Kırmızı Bitkileri Kitabı (Red Data Book of Turkish Plants). Türkiye Tabiatını Koruma Derneği Yayını Ankara, 178.

Ghafoor A. 2000. Genetic relationship in Vigna mungo (L.) Hepper and V. Radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica 123, 367–378.

Gillies ACM, Navarro C, Lowe AJ. 1999. Genetic diversity in Mesoamerican populations of mahogany (Swietenia macrophylla) assessed using RAPDs. Heredity 83, 722–732.

Goodman D. 1987. The demography of chance extinction in: Soule, M. E. (Eds) Viable Population for Conservation. Cambrige Univ. Press, Cambrigde.

Hamrick JL, Godt MJW. 1989. Allozyme diversity in plant species in: Brown AH, Clegg MT, Kahler AL, Weir BS. (Eds.) Plant population genetics, breeding, and genetic resources. Sinauer Associates, Sunderland.

Hamrick JL, Godt MJW. 1996. Conservation genetics of endangered plant species. In: Avise JC, Hamrick JL. (Eds.) Conservation genetics: case histories from nature. Chapman and Hall, London.

Hogbin PM, Ayre DJ, Whelan RJ. 1998. Genetic variation and reproductive success of road verge populations of the rare shrub Grevillea barklyana (Proteaceae). Heredity 80, 180–186.

Jian SG, Zhong Y, Liu N. 2006. Genetic variation in the endangered endemic species Cycas fairylakea (Cycadaceae) in China and implications for conservation. Biodiversity and Conservation 15 (5), 1681–1694.

Jolner SK, Sastad SM, Taberlet P. 2004. Amplified fragment length polymorphism versus random amplified polymorphic DNA markers: clonal diversity in Saxifraga cernua. Molecular Ecology 13, 81–8.

Laemli UK. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 277, 680–684.

Loveless MD, Hamrick JL. 1984. Ecological determinants of genetic structure in plant populations, Annual Review of Ecological Systems 15, 65–95.

Maguire TL, Sedgley M. 1997. Genetic diversity in Banksia and Dryandra (Proteaceae) with emphasis on Banksia cuneata a rare and endangered species. Heredity 79 (4), 394–401.

Marshall DR, Brown AHD. 1975. Optimum sampling strategies, genetic conservation, crop genetic resource for today and tomorrow in: Frankel OH, Hawkes JG. (Eds.) Cambrige Univ. Press, Cambrige.

Nybom H. 2000. Bartish IV. Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspectives in Plant Ecology, Evolution and Systematics (3), 93–114.

Nybom H. 2004. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology 13, 1143– 1155.

Okumuş A, Gülümser A. 2004. Determination of Genetic Diversity in Some Vetches (Vicia spp) by Seed Proteins. International Journal of Biology and Biotechnology 1(2), 149-152.

Özen F. 2006.  Türkiye’de  tükenme  tehlikesinde olan bir türün otekolojisi: Amsonia orientalis Decne. (Apocynaceae). BAÜ Fen Bilimleri Enstitüsü Dergisi 8, 1.

Premoli AC, Kitzberger T, Veblen TT. 2000. Conservation genetics of the endangered Conifer Fitzroya cupressoides in Chile and Argentina. Conservation Genetics 1 (1), 57–66.

Rahman AU, Zaman K. 1988. Studies on the alkaloids of Rhazya stricta., Phytochemistry 27 (6), 1926–1928.

Rahman AU, Qureshi MM, Zaman K. 1989. The alkaloids of Rhazya stricta and R. orientalis- A Review. Fitoterapia 60 (4), 291–322.

Sammour RH. 1991. Using electrophoretic techniques in varietal identification, biosystematic analysis, phylogenetic relations and genetic resources management. Journal of the Islamic Academy of Sciences 4 (3), 221–226.

Saraswati R, Matoh T. 1993. Identification of Sesbonia species from electrophoretic patterns of seed proteins. Tropical Agriculture Trinidad 70 (3), 282–285.

Shikano T, Shimada Y, Herczeg, G. 2010. History vs. habitat type: explaining the genetic structure of European nine-spined stickleback (Pungitius pungitius) populations. Molecular Ecology 19, 1147–1161.

Shrestha MK, Golan-Goldhirsh A, Ward D. 2002. Population genetic structure and the conservation of isolated populations of Acacia raddeana in the Negev Desert. Biological Conservation 108 (1), 119–127.

Surgun Y, Çöl B, Bürün B. 2012. Genetic diversity and identification of some Turkish cotton genotypes (Gossypium hirsutum L.) by RAPD-PCR analysis. Turkish Journal of Biology 36, 143–150.

Tutin TG, Heywood VH, Burges N.A, Moore DM, Valentine DH, Walters SM, Webb DA. 1968. Flora Europaea Vol. 2, Cambridge Univ. Press., England.

Wang JL, Zhao NX, Gai YB. 2006. RAPD analysis of genetic diversity and population genetic structure of Stipa krylovii Reshov. in inner Mongolia Stepe. Russian Journal of Genetics 42 (5), 468–475.

Waser NM. 1993. Population structure, optimal outbreeding, and assortative mating in angiosperms, the natural history of inbreeding and outbreeding in: Thornhill, NW, (Eds.) Theoretical and empirical perspectives, Univ. of Chicago, Chicago.

Williams JGK, Kubelik AR, Livak KJ. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.

Yeh FC, Yang RC, Boyle T. 1999 Microsoft Windows- based freeware for population genetic analysis. Release 1.32, University of Alberta, Edmonton.

Zahariadi C. 1973. Quelqes taxons rares ou nouvellement découverts de la flore de la Grèce. Annual Mus Goulandris 1, 165–183.

Zawko G, Krauss SL, Dixon KWL. 2001. Conservation Genetics of the Rare and Endangered Leucopogon obtectus (Ericaceae). Molecular Ecolology 10, 2389–2396.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background