Int. J. Biosci.2(9), 85-93, September 2012
This study was conducted to provide baseline information on the genetic population structure of Ompok bimaculatus, an endangered catfish of Bangladesh. Random amplified polymorphic DNA (RAPD) fingerprintinganalysis was performed to assess the genetic variation in two wild (Chalan beel and Tola haor) and one hatchery (Brahmmaputra Fish Seed Complex-BFSC) populations of O. bimaculatus,. Five selected decamer random primers amplified a total of 34 RAPD bands among which 24 were found to be polymorphic. The percentage of polymorphic loci, intra-population similarity indices, gene diversity and Shannon’s information indidex values were 64.71%, 77.57% , 0.249±0.216 and 0.365±0.303 for Chalan beel, 58.82%, 75.45%, 0.219±0.215 and 0.322±0.304 for Tola haor population and 52.94%, 86.49%, 0.214±0.219 and 0.311±0.312 for the hatchery, BFSC, respectively. The coefficient of population differentiation (PhiPT) between the Chalan beel – BFSC and Tola haor – BFSC pairs were found to be significant. The gene flow (Nm) between the population pairs ranged from1.899 to 5.052. The highest inter-population similarity (Sij) was found between Chalan beel-BFSC populations. Among the three populations, the highest genetic distance (0.157) was found between Tola haor and the BFSC population. The results of the present study indicated a substantial level of genetic variation in the enangerd O. bimaculatus populations in Bangladesh and significant differentiation among the populations..
Akter S, Sultana S, Khan MSR, Nahiduzzaman M, Hossain MAR, Alam MS. 2010. Genetic characterization of critically endangered Puntius sarana (Hamilton) and the exotic Barbonymus gonionotus (Bleeker) (Cyprinidae: Cypriniformes) by DNA Fingerprinting. International Journal of BioScience and Agriculture Technology 2, 21-27.
Alam MS, Islam MS, Alam MS. 2010. DNA fingerprinting of the freshwater mud eel, Monopterus cuchia (Hamilton) by Randomly Amplified Polymorphic DNA (RAPD) Marker. International Journal of Biotechnology and Biochemistry 6, 27-278.
Ambak M A, Bolong AM A, Ismail P, Tam BM. 2006. Genetic variation of snakehead fish (Channa striata) populations using random amplified polymorphic DNA. Biotechnology 5(1), 104-110.
Crozier RH. 1997. Preserving the information content of species: genetic diversity, phylogeny and conservation worth Annual Review of Ecology and Systematics 28, 243–268.
Garg RK, Sairkar P, Silawat N, Vijay N, Batav N, Mehrotra NN. 2009. Genetic diversity between two populations of Heteropneustes fossilis (Bloch) using RAPD profile. International Journal of Zoological Research 5, 171-177.
Garg RK, Sairkar P, Silawat N, Vijay N, Batav N, Mehrotra NN. 2010. Assessment of genetic diversity of Clarias batrachus using RAPD markers in three water bodies of Bhopal. Journal of Environmental Biology 31, 749-753.
Hassanien HA. 2008. Use of randomly amplified polymorphic DNA (RAPD) analysis to detect genetic variation in Seabass Dicentrarchus labrax. Journal of Fisheries and Aquatic Sciences 3, 39-46.
Islam MS, Alam MS. 2004. Randomly amplified polymorphic DNA analysis of four different populations of the Indian major carp, Labeo rohita (Hamilton). Journal of Applied Ichthyology 20, 407-412.
IUCN (International Union for Conservation of Nature). 2000. Red Book of Threatened Fishes of Bangladesh, IUCN- The World Conservation Union.
Kar D, Nagarathna AV, Ramachandra TV, Dey SC. 2006. Fish diversity and conservation aspects in an aquatic ecosystem in northeastern India. Zoo’s Print Journal 21, 2308-2315.
Khedkar GD, Reddy ACS, Persis M, Ravinder K, Muzumdar K. 2010. Clarias batrachus (Linn.1758) population is lacking genetic diversity in India. Molecular Biology Reports 37, 1355-1362.
Kimberling DN, Ferreira AR, Shuster SM, Keim P. 1996. RAPD marker estimation of genetic structure among isolated northern leopard frog populations in the south-western USA. Molecular Ecology 5, 521-529.
Liu ZJ, Li P, Argue BJ, Dunham RA. 1999. Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture 174, 59–68.
Loew SS. 2000. The role of genetics in conservation biology In: Quantitative Methods for Conservation Biology (eds. Ferson S, Burgman M), Springer Verlag, NY. p. 226–258.
Lynch M. 1990. The similarity index and DNA fingerprinting. Molecular Biology and Evolution 7, 478-484.
Lynch M, Milligan BG. 1994. Analysis of population genetic structure with RAPD markers. Molecular Ecology 3, 91-99.
Lynch M, Conery J, Burger R. 1995. Mutation accumulation and the extinction of small populations. American Naturalist 146, 489– 518.
Nei M. 1972. Genetic distance between populations. American Naturalist 106, 283-292.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences USA 70, 3321-3323.
Parween S. 2007. Ompok bimaculatus (Bloch, 1797). In: Siddiqui, K.U., Islam, M.A., Kabir, S.M.H., Ahmed, M., Ahmed, A.T.A., Rahman, A.K.A., Haque, E.U., Ahmed, Z.U., Begum, Z.N.T., Hasan, M.A., Khondker, M., and Rahman, M.M. (eds.). Encyclopedia of Flora and Fauna of Bangladesh, Vol. 23. Freshwater Fishes. Asiatic Society of Bangladesh, Dhaka. p.127.
Peakall R, Smouse PE. 2006. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology. Notes 6, 288-295.
Rahman AKA. 1989. Freshwater fishes of Bangladesh. The Zoological Society of Bangladesh. 168-169pp.
Rahman SMZ, Khan MR, Islam S, Alam MS. 2009. Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers. Genetics and Molecular Biology, 32, 197-201.
Saad YM, Hanafi MS, Essa MA, Guerges AA, Ali SF. 2009. Genetic signatures of some Egyptian Clarias gariepinus populations. Global Veterinarian 3, 503-508.
Sultana S, Akter S, Hossain MAR, Alam MS. 2010. DNA fingerprinting of the Asian stinging catfish (Heteropneustes fossilis, Bloch) by Random Amplified Polymorphic DNA markers. International Journal of Biotechnology Applications 2, 0975-9123.
Talwar PK, Jhingran AG. 1991. Inland fishes of India and adjacent countries. Oxford & IBH Publishing Co. PVT. Ltd., New Delhi, Vol. 2: 584pp.
Welsh J, McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 7213-7218.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531-6535.
Yeh FC, Yang RC, Boyle T. 1999. POPGENE version 1.31: Microsoft Window-based freeware for population genetic analysis. ftp://ftp.microsoft.com/Softlib/HPGI.EXE