Genetic variation in endangered butter catfish, Ompok bimaculatus (bloch) populations revealed by random amplified polymorphic DNA (RAPD) fingerprinting

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Genetic variation in endangered butter catfish, Ompok bimaculatus (bloch) populations revealed by random amplified polymorphic DNA (RAPD) fingerprinting

Jonaira Rashid, Faozia Mehbuba Tamanna, Mostafa Ali Reza Hossain, Md. Samsul Alam
Int. J. Biosci.2( 9), 85-93, September 2012.
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Abstract

This study was conducted to provide baseline information on the genetic population structure of Ompok bimaculatus, an endangered catfish of Bangladesh. Random amplified polymorphic DNA (RAPD) fingerprintinganalysis was performed to assess the genetic variation in two wild (Chalan beel and Tola haor) and one hatchery (Brahmmaputra Fish Seed Complex-BFSC) populations of O. bimaculatus,. Five selected decamer random primers amplified a total of 34 RAPD bands among which 24 were found to be polymorphic. The percentage of polymorphic loci, intra-population similarity indices, gene diversity and Shannon’s information indidex values were 64.71%, 77.57% , 0.249±0.216 and 0.365±0.303 for Chalan beel, 58.82%, 75.45%, 0.219±0.215 and 0.322±0.304 for Tola haor population and 52.94%, 86.49%, 0.214±0.219 and 0.311±0.312 for the hatchery, BFSC, respectively. The coefficient of population differentiation (PhiPT) between the Chalan beel – BFSC and Tola haor – BFSC pairs were found to be significant. The gene flow (Nm) between the population pairs ranged from1.899 to 5.052. The highest inter-population similarity (Sij) was found between Chalan beel-BFSC populations. Among the three populations, the highest genetic distance (0.157) was found between Tola haor and the BFSC population. The results of the present study indicated a substantial level of genetic variation in the enangerd O. bimaculatus populations in Bangladesh and significant differentiation among the populations..

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