J. Bio. Env. Sci.5(4), 23-30, October 2014
Genetic variation for 24 accessions of Agopyron trichophorm evaluated using 13 ISSR primers. In total 90 bands (loci) were obtained out of which 88 bands were polymorph in accessions studied. The primers of IS14, UBC808, UBC825, UBC835, UBC842 and UBC886 considering polymorphism information content (PIC) were useful for polymorphism study in Agropyron genus in the future researches. The similarity between accessions based on Dice’s coefficient was low; therefore there was a high genetic variation among accessions. The three groups of accessions obtained by the results of cluster analysis and principal coordinate analysis (PCo) which these results confirmed by molecular variance analysis. Grouping of accessions indicated that genetic variations do not in agreement with the geographical distribution of accessions. Finally, the accessions of the first group [Shahrekord (1), Semirom (14), Semirom (10), Mazandaran (23), Eghlid (19), Eghlid (20), Tehran (21), Esfahan (22) and Gorgan (24)] had the highest genetic distance with the accessions of the third group [Karaj (6), Bijar (7), Salmas (13), Semirom (15) and Yasouj (18)], based on ISSR markers. Therefore, considering the genetic distance between these groups, using of first and third group accessions can be useful in breeding programs, to utilization of heterosis.
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