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Genetic variation of Iranian barley landraces, commercial varieties and improved breeding lines using SSR markers

Homayoon Dadbeh, Seyed Siamak Alavi Kia, Sara Dezhsetan, Mohammad Moghaddam Vahed, Behzad Sadeghzadeh

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J. Bio. Env. Sci.7(2), 198-206, August 2015

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Abstract

In order to estimate the level of genetic diversity of 70 Iranian barley genotypes, commercial varieties and improved breeding lines, 100 microsatellite primers were employed. Genomic DNA was extracted from fresh leaves and amplification reactions were done by PCR. The amplification products were separated on 6% denaturing polyacrylamide gel. Seventy one primers amplified 290 alleles with the range of 2 to 15 and an average of 4.08 alleles per locus. The mean gene diversity, polymorphic information content (PIC) and frequency of major allele were 0.42, 0.38 and 0.68, respectively. Analysis of molecular variance based on separating genotypes into two groups (Iranian barley genotypes vs. commercial varieties and improved lines) indicated an acceptable genetic diversity between these groups. Furthermore, larger proportion of within group variation (88%) of the total molecular variance determined the presence of high genetic variation within the groups. Nei’s gene diversity index and Shannon’s index were 0.4 and 0.75 for Iranian barley genotypes and 0.39 and 0.7 for commercial and improved lines, respectively, indicating the existence of reliable genetic diversity within both groups. Cluster analysis based on Minimum Evolution algorithm and Jukes-Cantor distance coefficient assigned the genotypes into three groups and within a group. In conclusion based on the molecular data analysis, substantial amount of genetic diversity was observed among the barley genotypes under study which can be utilized in breeding programs for selecting and producing superior barley lines.

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Genetic variation of Iranian barley landraces, commercial varieties and improved breeding lines using SSR markers

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