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Genotyping of Methicillin-resistant Staphylococcus aureus with rep-PCR

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Research Paper 01/06/2017
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Genotyping of Methicillin-resistant Staphylococcus aureus with rep-PCR

Aasma Akhtar, Naima Mahreen, Javaria Altaf, Muhammad Saqib, Mian Abdul Hafeez, Faisal Saeed Awan
Int. J. Biosci.10( 6), 265-275, June 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: Genetic diversityMethicillin-resistantRepetitive sequence based PCRStaphylococcus aureus

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is a significant infectious agent which cause infections in human and animals. This study was aimed to genotype human MRSA isolates recovered from wounds. Of the 66 wound cultures, 32 were confirmed as S. aureus and 23 had given positive signal MRSA, respectively. There were consistent banding patterns among all 23 MRSA isolates. The dendrogram attained from the Rep and BOX analysis showed 2 major groups showing a little bit maximum and distant relationship among MRSA strains. Henceforth, this technique could be of valuable use in scheming the modes as well as sources of transmission and mainly inhibiting the hospital related infections those are caused by the MRSA strains.

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