Germination response of endangered medicinal plant, Levisticum officinale, to stratification and some plant growth regulators

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Research Paper 01/09/2015
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Germination response of endangered medicinal plant, Levisticum officinale, to stratification and some plant growth regulators

Mansooreh Khodashenas, Batool Keramat, Yousef Emamipoor
J. Bio. Env. Sci.7( 3), 228-235, September 2015.
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Levisticum officinale is an important and endangered wild medicinal plant belongs to Apiaceae family. Pre experiment showed that seeds of this plant have some degree of dormancy and its germination is very low. Therefore, a factorial experiment laid out in completely randomized design with 3 replications to evaluate the effect of different plant growth regulators including: gibberellic acid (GA3), methyl jasmonate (MeJA), salicylic acid (SA) and stratification (4˚ C for 30 days) to alleviate seed dormancy. Seed germination percentage, germination rate, means germination time, seed vigor index and seedling growth traits were recorded during experiment. The findings indicated that stratification or application of plant growth regulators alone could improve germination partially, but their effect was not considerable, while application of plant growth regulators combined to stratification was more effective. The maximum germination percentages (77.66%), the maximum of shoot dry weight (4.2 mg) and root dry weight (1.4 mg) achieved when 10 µM of MeJA was applied. However, for the other studied traits 2000 mg/l GA3 was the best treatment. The results suggest that L.officinale has dormancy which can help the plant for addaptation in cold climate. Application GA 2000 mg/l in combination to stratification is recommended in order to improve germination characters.


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