Green synthesis of silver nanoparticles by using bacterial extract and its antimicrobial activity against pathogens

Paper Details

Research Paper 01/11/2018
Views (362) Download (15)

Green synthesis of silver nanoparticles by using bacterial extract and its antimicrobial activity against pathogens

Ihsan Ali, Tian Yong Qiang, Nikhat Ilahi, Mian Adnan, Wasim Sajjad
Int. J. Biosci.13( 5), 113-127, November 2018.
Certificate: IJB 2018 [Generate Certificate]


Nanotechnology is the amendment, alteration, and development of significant properties of metals in the form of nanoparticles having applications in numerous fields. This study was conducted to synthesize functionalized silver nanoparticles (AgNPs) using bacterial T10 strain isolated from unusual environment. Stable and well dispersed AgNPs were obtained extracellularly by using bacterial extract within 1 hour of incubation. Reduction of silver ion was checked by using UV–visible spectrophotometry, and characterization of the AgNPs were done by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction(XRD). These nanoparticles were monodispersed, spherical and about 46–52.7 nm in size. XRD peaks were corresponding to diffraction facets of silver planes. Capping of AgNPs with functional groups were confirmed through FTIR. The obtained AgNPs showed antimicrobial against clinical pathogens and synergistic effect with antibiotics. Antibacterial activity of obtained AgNPs was tested against clinical pathogens by coating AgNPs on bandage. Our results clearly proposethe bacterial extractsis anexcellent source for green synthesis of AgNPs and could be used against pathogens.


Balakumaran M, Ramachandran R, Balashanmugam P, Mukeshkumar D, Kalaichelvan. 2016. Mycosynthesis of silver and gold nanoparticles optimization characterization and antimicrobial activity against human pathogens. Microbiological Research 182, 8–20.

Basavaraja S, Balaji S, Lagashetty A, Rajasab Z, Venkataraman A. 2008. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. MaterialsResearch Bulletin 43, 1164−1170.

Batal EL, Amin AI, Shehata MA, Merehan MAH. 2013. Synthesis of silver nanoparticles by Bacillus stearothermophilus using gamma radiation and their antimicrobial activity. World Applied Sciences Journal 22, 1–16.

Dong ZY, Manik PNR, Min Xiao, Hong-Fei W, Wael N, Hozzein Wei C, Wen-Jun L. 2017. Antibacterial activity of silver nanoparticles against Staphylococcus warneri synthesized using endophytic bacteria by photo-irradiation. Frontiers in Microbiology 8, 1-8.

Duran N, Marcarto P, Souza D, Alves GIH, Esposito E. 2007. Antibacterial effect of silver nanoparticles produced by fungal process on textile fabrics and their effluent treatment. Journal Biomedical Nanotechnology 3, 203–208.

El-Rafie M, Ahmed HB, Zahran M. 2014.  Characterization of nano-silver coated cotton fabrics and evaluation of its antibacterial efficacy. Carbohydrate Polymers 107, 174−181.

Emam HE, Saleh N, Nagy KS, Zahran M. 2015. Functionalization of medical cotton by direct incorporation of silver nanoparticles. International Journalof Biological Macromolecules 78, 249−256.

Fayaz AM, Balaji K, Girilal M, Yadav R, Kalaichelvan PT, Venketesan R. 2010. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics a study against gram-positive and gram-negative bacteria. Nanomedicine Nanotechnology Biology and Medicine 6, 103−109.

Galib BM, Mashru M, Jagtap C, Patgiri BJ, Prajapati PK.2011. Therapeutic potentials of metals in ancient India: A review through Charaka Samhita. Journal of Ayurveda and Integrative Medicine 2, 55−63.

Jain D, Kachhwaha S, Jain R, Srivastava G, Kothari LS. 2010. Novel microbial route to synthesize silver nanoparticles using spore crystal mixture of bacillus thuringiensis. Indian Journal of Experimental Biology 48(11), 1152–1156.

Kharissova OV, Rasika HV, Kharisov BI, Perez BO, Perez VMJ. 2013. The greener synthesis of nanoparticles. Trends in Biotechnology 31(4), 240–248.

Klaus T, Joerger R, Olsson E, Granqvist CG. 1999. Silver based crystalline nanoparticles microbially fabricated. Proceedings of the National Academy of Sciences of the United States of America 96(24), 13611–13614.

Lengke MF, Fleet ME, Southam G. 2007. Biosynthesis of silver nanoparticles by filamentous cyanobacteria from a silver(I) nitrate complex. Langmuir 23(5), 2694– 2699.

Manikprabhu D, Lingappa K. 2013. Antibacterial activity of silver nanoparticles against methicillin-resistant Staphylococcus aureus synthesized using model Streptomyces sp. pigment by photo-irradiation method. Journal of Pharmacy Research 6, 255–260.

Mody VV, Siwale R, Singh A, Mody HR.2010. Introduction to Metallic Nanoparticles. Journal of Pharmacy and Bio-allied Sciences 2, 282−289.

Mohanpuria P, Rana NK, Yadav SK. 2008. Biosynthesis of nanoparticles technological concepts and future applications. Journal of Nanoparticle Research 10(3), 507–517.

Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ. 2005. The bactericidal effect of silver nanoparticles. Nanotechnology 16(10), 2346–2353.

Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Parishcha R, Ajaykumar PV, Alam M, Kumar R, Sastry M. 2001. Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix a novel biological approach to nanoparticle synthesis. Nano Letters 1(10), 515–519.

Nambiar D, Bhathena ZP. 2010. Use of silver nanoparticles from Fusarium oxysporum in wound dressings. Journal of Pure and Applied Microbiology 4(1), 207–214.

Netala VR, Kotakadi VS, Bobbu P, Gaddam SA, Tartte V. 2016. Endophytic fungal isolate mediated biosynthesis of silver nanoparticles and their free radical scavenging activity and antimicrobial studies. 3 Biotech 6, 132.

Panáček A, Kvitek L, Prucek R, Kolář M, Večeřová R, Pizúrová N, Sharma VK, Nevěčná TJ, Zbořil R. 2006. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. The Journal of Physical Chemistry B 110(33), 16248–16253.

Poulose S, Panda T, Nair PP, Theodore T. 2014. Biosynthesis of silver nanoparticles. Journal of Nanoscience and Nanotechnology 14(2), 2038–2049.

Priyadarshini S, Gopinath V, Meera Priyadharsshini N, MubarakAli D, Velusamy. 2013. Synthesis of anisotropic silver nanoparticles using novel strain Bacillus flexus and its biomedical application. Colloids and Surfaces B Bio-interfaces 102, 232–237.

Qian Y, Yu H, He D, Yang H, Wang W, Wan X, Wang L. 2013. Biosynthesis of silver nanoparticles by the endophytic fungus Epicoccum nigrum and their activity against pathogenic fungi Bioprocess. Bioprocess Biosystem Engineering 36, 1613–1619.

Rahim KAAA, Mohamed AMA. 2015. Bactericidal and antibiotic synergistic effect of nano-silver against methicillin-resistant Staphylococcus aureus. Jundishapur Journalof Microbiology 8(11).

Rai M, Deshmukh S, Ingle A, Gade A. 2012. Silver nanoparticles the powerful nanoweapon against multidrug-resistant bacteria. Journal of Applied Microbiology 112, 841-852.

Schrofel G, Kratosova I, Safarık M, Safarıkov I, Raska L, Shor M. 2014. Applications of biosynthesized metallic nanoparticles-a review. Acta Biomaterialia 10(10), 4023–4042.

Shahverdi R, Minaeian S, Shahverdi H, Jamalifar, Nohi AA. 2007. Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria a novel biological approach. Process Biochemistry 42(5), 919–923.

Sharma VK, Yngard RA, Lin Y. 2009. Silver Nanoparticles Green Synthesis and Their Antimicrobial Activities. Advances in Colloid and Interface Science 145, 83−96.

Shivaji S, Madhu S, Singh S. 2011. Extracellular synthesis of antibacterial silver nanoparticles using psychrophilic bacteria. Process Biochemistry 46(9), 1800–1807.

Singh M, Singh S, Prasad S, Gambhir I. 2008. Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Journal of Nanomaterials and Biostructures 3, 115-122.

Singh R, Singh D. 2014.Chitin membranes containing silver nanoparticles for wound dressing application. International Wound Journal 11(3), 264–268.

Sunkar S, Nachiyar CV. 2012. Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus. Asian Pacific Journalof Tropical Biomedicine 2, 953–959.

Tamura K, Nei M. 1993.Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10, 512−526.

Tian J, Wong KK, Ho CM, Lok CN, Yu WY, Che CM, Chiu JF, Tam PK. 2007. Topical delivery of silver nanoparticles promotes wound healing. Chem Med Chem 2(1), 129–136.