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Research Paper | November 1, 2018

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Green synthesis of silver nanoparticles by using bacterial extract and its antimicrobial activity against pathogens

Ihsan Ali, Tian Yong Qiang, Nikhat Ilahi, Mian Adnan, Wasim Sajjad

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Int. J. Biosci.13(5), 1-15, November 2018

DOI: http://dx.doi.org/10.12692/ijb/13.5.1-15


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Nanotechnology is the amendment, alteration, and development of significant properties of metals in the form of nanoparticles having applications in numerous fields. This study was conducted to synthesize functionalized silver nanoparticles (AgNPs) using bacterial T10 strain isolated from unusual environment. Stable and well dispersed AgNPs were obtained extracellularly by using bacterial extract within 1 hour of incubation. Reduction of silver ion was checked by using UV–visible spectrophotometry, and characterization of the AgNPs were done by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR)and X-Ray Diffraction(XRD). These nanoparticles were mono-dispersed, spherical and about 46–52.7 nm in size. XRD peaks were corresponding to diffraction facets of silver planes. Capping of AgNPs with functional groups were confirmed through FTIR. The obtained AgNPs showed antimicrobial against clinical pathogens and synergistic effect with antibiotics. Antibacterial activity of obtained AgNPs was tested against clinical pathogens by coating AgNPs on bandage. Our results clearly propose the bacterial extracts is an excellent source for green synthesis of AgNPs and could be used against pathogens.


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