Growth characteristics of in vitro plantlets of Hevea brasiliensis obtained from immature embryo culture
Paper Details
Growth characteristics of in vitro plantlets of Hevea brasiliensis obtained from immature embryo culture
Abstract
Immature pods of Hevea brasiliensis were generated through controlled pollination at the Rubber Research Institute of Nigeria for the purpose of embryo rescue. The target was to enhance Hevea breeding success in the development of hybrid materials. The pods at ten (10) and twelve (12) weeks old were collected and cultured in vitro for 8weeks in 2009 and 2010. Pods were surface sterilized, embryos were isolated and cultured in vitro using MS, GB5) and SH media. At eight weeks in culture, plantlets were taken to the screen house, removed from the vessels and evaluated for growth characteristics: No of leaves, shoot length (cm), No of roots and root length (cm) and were subjected to statistical analysis. The results showed that there was significant variation among the six genotypes in 2009 but no significant difference in 2010 across the parameters determined. Similarly, the three in vitro growth media did not differ significantly from one another across the parameters for the two years of the study. With respect to the age of the embryos, 12 weeks old embryos significantly out performed those of the 10 weeks old embryos. Generally, embryos significantly had better performance in 2010 than in 2009. Based on the findings from this study, any of the three growth media can be used successfully for the germination of Hevea immature embryos in vitro. Also, since the older embryos had better performance than those from the younger embryos, age of immature Hevea embryo is a significant factor for consideration to ensure better growth performance in vitro.
Burun B, Poyrazoglu CE. 2002. Embryo culture in Barley (Hordium vulgare L.). Turkey Journal of Biology 26, 175 – 180.
Chen Z. 1990. Rubber (Hevea brasiliensis Muell. Arg.): In vitro production of haploids. In: Biotechnology in Agriculture and Forestry, Haploid in crop improvement I. Y. P. S. Bajaj (Ed). Springer-verlag. Paris 12, 215 – 235.
Chopra VL, Sharma RD. 1989. Innovative approaches for crop improvement. In: Plant breeding theory and practice, V. L. Chopra (Ed.), Oxford & IBH publishing Co. PVT. LTD. New Delhi. 453 – 466.
Fratini R, Ruiz ML. 2006. Interspecific Hybridization in the genus Lens applying in vitro embryo rescue. Euphytica 150, 271 – 280.
Gamborg OI, Miller RA, Ojima K. 1968. Nutrient requirement of suspension culture of soybean root cells. Experimental Cell Resources 50, 151 – 158.
Gomez KA, Gomez AA. Statistical procedures for Agricultural Research. 2nd Edition. John Wiley & Son. New York. 680p
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum. 15, 473 – 497.
Mydin KK, Annamma V, Nazeer MA, Premakumari D, Saraswathyamma CK, Panikkar AON. 1990. Controlled pollination in Hevea: Problems and perspectives. Proceeding of IRRDB Breeding Symposium, 1990. Kunming, China.
Ng SYC. 2002. Postflask management of cassava and yam. International Institute for Tropical Agriculture (IITA) Research Guide 69, 22.
Niederwieser IG. 2004. Role of Biotechnology in the development and production of Lachenalia and ornithogalum cultivars in South Africa. South African Journal of Botany 70, 47 – 51. doi:10.1016/S0254-6299(15)30267-2 doi:10.1016/S0254-6299(15)30267-2 doi:10.1016/S0254-6299(15)30267-2 doi:10.1016/S0254-6299(15)30267-2
Nunez-Palenius HG, Klee HJ, Cantliffe DJ. 2006. Embryo-rescue culture of the ‘Galia’ muskmelon (Cucumis melo L. var. reticulates Ser.) Male line. Plant Cell, Tissue and Organ culture 85, 345 – 352.
Omokhafe KO, Akpobome FA, Nasiru I. 2007. Diallel analysis of fruit set in Hevea brasiliensis muell. Arg. Genetics and Molecular Biology 30(2), 428 – 430.
Palmer JL, Lawn RL, Adkins SW. 2002. An embryo-rescue protocol for Vigna interspecific hybrids. Australian Journal of Botany 50, 331 – 338.
Rodrigues-Otubo BM, Penteado MI de O, Do Valle CB. 2000. Embryo rescue of interspecific hybrids of Brachiaria ssp. Plant Cell, Tissue and Organ culture 61, 175 – 182.
Schenk RV, Hildebrandt AC. 1972. Medium and techniques for induction of growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany 50, 166 – 204.
Sharma DR, Kuar R, Kumar K. 1996. Embryo rescue in plants – a review. Euphytica 89, 335 – 337.
Werbrouch POS, Debergh PC. 1994. Applied aspects of plant regeneration. In: Plant Cell Culture, a practical approach. Dixon, R. A. and Gonzales, R. A. (Eds.). Second edition. Oxford University Press. New York, 127 – 134 P.
F. A. Akpobome, J.K. Mensah, K. O. Omokhafe, E. E. Omo-Ikerodah, I. K. Okore, 2017. Growth characteristics of in vitro plantlets of Hevea brasiliensis obtained from immature embryo culture. Int. J. Biosci., 11(1), 1-6.
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