Growth characteristics of in vitro plantlets of Hevea brasiliensis obtained from immature embryo culture

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Research Paper 01/07/2017
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Growth characteristics of in vitro plantlets of Hevea brasiliensis obtained from immature embryo culture

F. A. Akpobome, J.K. Mensah, K. O. Omokhafe, E. E. Omo-Ikerodah, I. K. Okore
Int. J. Biosci.11( 1), 1-6, July 2017.
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Immature pods of Hevea brasiliensis were generated through controlled pollination at the Rubber Research Institute of Nigeria for the purpose of embryo rescue. The target was to enhance Hevea breeding success in the development of hybrid materials. The pods at ten (10) and twelve (12) weeks old were collected and cultured in vitro for 8weeks in 2009 and 2010. Pods were surface sterilized, embryos were isolated and cultured in vitro using MS, GB5) and SH media. At eight weeks in culture, plantlets were taken to the screen house, removed from the vessels and evaluated for growth characteristics: No of leaves, shoot length (cm), No of roots and root length (cm) and were subjected to statistical analysis. The results showed that there was significant variation among the six genotypes in 2009 but no significant difference in 2010 across the parameters determined. Similarly, the three in vitro growth media did not differ significantly from one another across the parameters for the two years of the study. With respect to the age of the embryos, 12 weeks old embryos significantly out performed those of the 10 weeks old embryos. Generally, embryos significantly had better performance in 2010 than in 2009. Based on the findings from this study, any of the three growth media can be used successfully for the germination of Hevea immature embryos in vitro. Also, since the older embryos had better performance than those from the younger embryos, age of immature Hevea embryo is a significant factor for consideration to ensure better growth performance in vitro.


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