Heavy metals bioaccumulation in muscle tissues of Catla catla, Labeo rohita and Cirrhinus mrigala exposed to Chakbandi drain water concentrations in glass aquaria

Paper Details

Research Paper 01/12/2017
Views (283) Download (12)

Heavy metals bioaccumulation in muscle tissues of Catla catla, Labeo rohita and Cirrhinus mrigala exposed to Chakbandi drain water concentrations in glass aquaria

Muhammad Arshad Rana, Tayyaba Sultana, Salma Sultana, Bilal Hussain
J. Bio. Env. Sci.11( 6), 160-170, December 2017.
Certificate: JBES 2017 [Generate Certificate]


This project was planned to study the heavy metals bioaccumulation in muscle tissues of Catla catla, Labeo rohita and Cirrhinus mrigala exposed to Chakbandi drain water concentrations in glass aquaria. In this study, Chakbandi drain’s composite water were collected from the selected sites in the month of April, May and June, 2016 and applied to fingerling’s of three fish species i.e. Catla catla, Labeo rohita and Cirrhinus mrigala under laboratory conditions in glass aquaria. After determining the LC50, the sub-lethal dilutions i.e. 20%, 25%, 30%, 35% and 40% of drain water were tested for three month’s acute toxicity trial. After three months, muscle tissues of all the three fish species were taken by dissecting the control and experimental fishes for heavy metals bioaccumulation studies. Bioaccumulation of Cu, Cr, Mn, Ni, Cd, Co, Sn, Hg, Zn and Pb was detected in all the drain water treated fish species. Among all the treated fish species Cirrhinus mrigala showed the maximum bioaccumulation of all the selective heavy metals as compared to Labeo rohita and Catla catla. Higher level of bioaccumulation of Mn, Cu and Pb was detected in the muscle tissues of Cirrhinus mrigala as compared to other treated fish species. Conclusively, bioaccumulation of heavy metals in fish species is reliable tool for the assessment of environmental pollution. The findings of this study are helpful as an early warning for environmental monitoring strategies and for the evaluation of eco-toxicological impacts of pollutions.


Adeyemo, O. K. 2005. Haematological and histopathological effects of Cassava Mill Effluent in Clarias gariepinu. African Journal of Biomedical Research 8, 179-183.

Al-Sabti K, Metcalfe CD. 1995. Fish micronuclei for assessing genotoxicity in water. Mutation Research 343(2), 121-35.

Ambreen F, Javed M, Batool U. 2015. Tissue specific heavy metals uptake in economically important fish, Cyprinus carpio at acute exposure of metals mixtures. Pakistan Journal of Zoology 47(2), 399-407.

APHA. 1998. Standard methods for the examination of water and wastewater. (14th Ed.) Washington pp. 1193-1195.

Bolognes, C, Cirillo S. 2014. Genotoxicity biomarkers in aquatic bio indicators. Current Zoology 60(2), 273-284.

Bonga WSE. 1997. The stress response in fish. Physiology Revolution 77, 591-625.

Cid BP, Boia C, Pombo L, Rebelo E. 2001. Determination of trace metals in fish species of the Riade aveiro (Portugal) by electro thermal atomic absorption spectrometry. Food chemistry toxicology 75, 93-100.

Ginebreda A, Kuzmanovic M, Guasch H, López de Alda M, López-Doval JC. 2014. Assessment of multi-chemical pollution in aquatic ecosystems using toxic units: Com-pound prioritization, mixture characterization and relationships with biological descriptors. Science Total Environment 469, 715-723.

Gupta V, Malik DS, Dinesh K. 2017. Risk assessment of heavy water pollution in middle stretch of river Ganga: An introspection. International Research Journal of Environment Science 6(2), 62-71.

Hayat S, Javed M, Razzaq S. 2007. Growth performance of metal stressed major carps viz. Catla catla, Labeo rohita and Cirrhinus mrigala reared under semi-intensive culture system. Pakistan Veterinary Journal 27(1), 8-12.

Jamdade AB, Gawande SM. 2017. Analysis of water quality parameters. International Journal of Engineering Research 6(3), 145-148.

Javed M. 2005. Growth responses of Catla catla, Labeo rohita and Cirrhina mrigala for bio-accumulation of zinc during chronic exposure. Pakistan Journal of Biological Science 8, 1357-1360.

Junianto, Zahidah & Apriliani IM. 2017. Evaluation of heavy metal contamination in various fish meat from Cirata Dam, West Java, Indonesia. AACL Bioflux, 10(2), 711-717.

Lee HB, Peart TE. 2000. Bisphenol A contamination in Canadian municipal and industrial wastewater and sludge samples. Water Quality Research Journal of Canada 35, 283-298.

Naz S, Javed M. 2013. Growth responses of fish during chronic exposure of metal mixtures under laboratory conditions. Pakistan Veterinary Journal 33, 354-357.

Noor N, Zutshi B. 2016. Bioaccumulation of trace metals in tissues of rohu fish for environmental risk assessment. Journal of Water Resource and Protection 8, 472-481.

Qureshi AL, Asghar A, Mahessar, Leghari ME, Lashari BK, Mari FM. 2015. Impact of releasing wastewater of sugar industries into drainage system of LBOD, Sindh, Pakistan. International Journal of Environmental Science and Development 6(5), 381-386.

Rauf A Javed M, Ubaidullah M. 2009. Heavy metal levels in three major carps (Catla catla, Labeo rohita and Cirrhina mrigala) from the river Ravi, Pakistan. Pakistan veterinary Journal 29(1), 24-26.

Ruane NM, Bonga WSE, Balm PHM. 1999. Differences between rainbow trout and brown trout in the regulation of the pituitary inter-renal axis and physiological performance during confinement. Comparative Endocrinology 115, 210-219.

Sabullah MK, Ahmad SA, Shukor MY, Gansau AJ, Syed MA, Sulaiman MR, Shamaan NA. 2015. Heavy metal biomarker: Fish behavior, cellular alteration, enzymatic reaction and proteomics approaches. International Food Research Journal 22(2), 435-454.

Shakir HA, Qazi JI, Chaudhry AS. 2015. Assessing human health risk of metal accumulations in a wild carp fish from selected sites of a river loaded with municipal and industrial wastes. International Journal of Environmental Research 9(2), 545-552.

Sultana T, Siddique A, Sultana S, Mahboob S, Al-Ghanim K, Ahmed Z. 2016. Fish scales as a non-lethal tool of the toxicity of wastewater from the River Chenab. Environment Science and Pollution Research 24 (3), 2464-2475.

Thangam Y. 2014. Histopathological studies on nitrite toxicity to freshwater fish, Cirrhinus mrigala. Journal of Environmental Science, Toxicology and Food Technology 8(4), 10-14.