Hepatitis treatment potential of Echeveria elegans extract in Mus musculus var. albino with CCl4-induced liver injury

Paper Details

Research Paper 04/05/2024
Views (461) Download (27)
current_issue_feature_image
publication_file

Hepatitis treatment potential of Echeveria elegans extract in Mus musculus var. albino with CCl4-induced liver injury

Bao-Ngan Dang, Thi-Phuong-Nga Pham, Nguyen-Hoang-Uyen Tran, Hoang-Gia-Lac Vo,Van-Thanh Vo
Int. J. Biosci.24( 5), 50-57, May 2024.
Certificate: IJB 2024 [Generate Certificate]

Abstract

This study investigated the potential hepatoprotective effects of Echeveria elegans extract against CCl4-induced liver injury in albino mice (Mus musculus var. albino). Mice were divided into 6 groups: control, E. elegans extract 100% (SD100), CCl4, silymarin (standard drug), CCl4 + E. elegans extract 25% (CCl4.SD25), and CCl4 + E. elegans extract 50% (CCl4.SD50). After 6 weeks, growth performance parameters like weight gain, average daily gain, and specific growth rate were measured. Serum levels of AST, ALT, and bilirubin were analyzed as biochemical indicators of liver function. Histological examination of liver tissues was also performed. The CCl4 group showed signs of liver injury like increased liver size, pale color, and white spots. However, the E. elegans extract groups, especially CCl4.SD50 demonstrated significant improvement in gross liver morphology and histological architecture, comparable to the silymarin group. While serum enzyme levels did not differ significantly between groups.The CCl4.SD50 group showed a greater reduction in bilirubin levels than silymarin. The findings suggest that E. elegans extract, particularly at 50% concentration, exhibits promising hepatoprotective potential against CCl4-induced chronic liver injury, warranting further investigation into its therapeutic efficacy and mechanism of action.

VIEWS 84

Crespo Yanguas S, Cogliati B, Willebrords J, Maes M, Colle I, Van Den Bossche B, De Oliveira CPMS, Andraus W, Alves VA, Leclercq I, Vinken M. 2016. Experimental models of liver fibrosis. Archives of Toxicology 90(5), 1025–1048. https://doi.org/10.1007/s00204-015-1543-4

Duong TPL, Cao TKH, Nguyen TH, Xuan C, Phan TBT, Ha TT. 2016. Hepatoprotective effect of silymarin on chronic hepatotoxicity in mice induced by carbon tetrachloride. Journal of Pharmacognosy and Phytochemistry 5(5), 262–266.

Elmasry S, Moawad M. 2021. The hepatoprotective effect of gooseberry and black mulberry extracts against carbon tetrachloride-induced liver injury in rats. The Journal of Basic and Applied Zoology 82(1), 33. https://doi.org/10.1186/s41936-021-00224-z

Elnfarawy AA, Nashy AE, Abozaid AM, Komber IF, Elweshahy RH, Abdelrahman RS. 2021. Vinpocetine attenuates thioacetamide-induced liver fibrosis in rats. Human and Experimental Toxicology 40, 355–368. https://doi.org/10.1177/0960327120947453

Gowifel AM, Khalil MG, Nada SA, Kenawy SA, Ahmed KA, Salama MM, Safar MM. 2020 Combination of pomegranate extract and curcumin ameliorates thioacetamide-induced liver fibrosis in rats: impact on TGF-β/Smad3 and NF-κB signaling pathways. Toxicology Mechanisms and Methods 30, 620–633. https://doi.org/10.1080/15376516.2020.1801926

Lieber CS. 1990. Mechanism of ethanol induced hepatic injury. Pharmacology & Therapeutics 46(1), 1–41. https://doi.org/10.1016/0163-7258(90)90032-W

López-Angulo G, Díaz-Camacho SP, Heredia-Mercado B, Montes-Avila J, Delgado-Vargas F. 2022. Adaptogenic, immunomodulatory, and antioxidant activities of three Echeveria species (Crassulaceae). South African Journal of Botany 151, 255–262. https://doi.org/10.1016/j.sajb.2022.10.001

Mi XJ, Hou JG, Jiang S, Liu Z, Tang S, Liu XX, Wang YP, Chen C, Wang Z, Li W. 2019. Maltol mitigates thioacetamide-induced liver fibrosis through TGF-β1-mediated activation of PI3K/Akt signaling pathway. J. Agric. Food 67, 1392–1401. https://doi.org/10.1021/acs.jafc.8b05943

Nair SKP, Ganesan K, Sinaga M, Letha N, Gani B. 2016. Preliminary phytochemical screening of different solvent extracts of leaves of Echeveria elegans rose, an endangered mexican succulent herb. 5(1), 3429-3432.

Nguyen NL, Hoang TBN, Nguyen TH, Do TT, Ta VT, Pham TN. 2012. Liver biochemistry. In Biochemistry (pp. 276–288). Medicine.

Nguyen V, Vu CH, Vi HT, Trinh QH, Le DR, Le DH, Chan VH, Nguyen VP. 2005. Liver anatomy. In Pathological natomy (3rd ed.). Medicine.

Phan KD, Dai TXT, Nguyen TT. 2019. Antioxidant and hepatoprotective activities of the methanol leaf extract of Neolamarckiacadamba (Roxb.) Bosser. Can Tho University Journal 55(5), 24. https://doi.org/10.22144/ctu.jvn.2019.124

Phan KD, Dai TXT, Nguyen TT, Nguyen TTL. 2018. Study on hepatoprotective activity of the methanol leaf extract of skunkvine (Paederia scandens L.) on carbon tetrachloride – induced hepatic damage in mice. Can Tho University Journal 54(7A), 94–100. https://doi.org/10.22144/ctu.jvn.2018.128

Phan KD, Truong DYA, Truong TTT, Dai TXT. 2016. Study on hepatoprotective effect of root methanolic extract of Acanthus ilicifolius L. in carbon tetrachloride induced mice. Vietnam Journal of Biotechnology 14(2), 253–259. https://doi.org/10.15625/1811-4989/14/2/9338

Shareef SH, Ibrahim IAA, Alzahrani AR , Al-Medhtiy MH, Abdulla MA. 2022. Hepatoprotective effects of methanolic extract of green tea against thioacetamide-induced liver injury in Sprague Dawley rats. Saudi Journal of Biological Sciences 29, 564-573. https://doi.org/10.1016/j.sjbs.2021.09.023

Tanquilut, N C and Sanchez, G C and Gopes, M C B and Tapnio, R N and Tanquilut, M R C and Soriano, H M and Mula, M G Mula, RP. 2015. Growth and reproductive performance of white mice (Mus musculus Linn.) as influenced by pigeonpea (Cajanus cajan(L.) Millsp.). Green Farming 6(6), 1357-1360.

Tsai MY, Yang WC, Lin CF, Wang CM, Liu HY, Lin CS, Lin JW, Lin WL, Lin TC, Fan PS. 2021. The ameliorative effects of fucoidan in thioacetaide-induced liver injury in mice. Molecules 26, 1937. https://doi.org/10.3390/molecules26071937