Hepato-protective capacity of Morus (Morus macroura) fruit extract against Lead treated mice: A histopathological study

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Research Paper 01/02/2020
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Hepato-protective capacity of Morus (Morus macroura) fruit extract against Lead treated mice: A histopathological study

Iram Inayat, Syeda Nadia Ahmad, Iqra Muqaddas, Sadia Suleman, Saira Siddique, Aima Iram Batool, Mehwish Nasir, Muhammad Ali Kanwal, Khawaja Raees Ahmad
Int. J. Biosci.16( 2), 485-495, February 2020.
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Present research work focuses on hepatotoxicity of lead exposure and hepato-regenerative potential of Morus macroura Black (MMB) fruit extract post treatment. 15 male mice were distributed into three groups (n=5) that is, 1:  CO group (without any treatment), 2:  Pb group (50 ppm lead ion in lead acetate dissolved in drinking water), 3:  Pb+MMB group (exposure to lead as in the Pb group + 5 days treatment with 0.2ml MMB fruit extract once per 24 hours). Animals in all three groups were recovered on 21st day. Animal and organ weight and various other parameters (CSA of hepatocytes, number of hepatocytes per unit area, number of oval cells per unit area, Mean CSA of central hepatic vein, CSA of hepatic nuclei) were assessed. Result has shown severe liver damage indications such as shrinkage of sinusoidal spaces, swelling and enlargement of hepatocytes and presence of debris around the marginal hepatic portal venules as significant alterations in lead exposure. Significant recovery of above said parameters and their histological signs are indicated by presence of oval cells, large numbers of macrophages, peri-central area containing normal hepatocytes and enlargement in the size of both marginal & central hepatic vein were observed in MMB post treatment in Pb+MMB group. Lead is highly toxic to the animal liver and morus fruit extract has shown excellent recovery potential from lead exposure related toxicity. Present study has highlighted the need to further explore the potential of morus fruit extract in hepatotoxicity in particular and rest of body in general so as its medicinal capacity may be understood for the benefit of humanity.


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