Paper Details

Heterosis investigation of sunflower (Helianthus annuus L.) by two-dimensional electrophoresis

Sanaz Adalatzadeh-Aghdam, Mahmoud Toorchi, MomamadReza Shakiba

DOI: https://dx.doi.org/10.12692/ijb/4.10.70-79

Int. J. Biosci. 4(10), 70-79. May, 2014. (PDF)

Abstract:

Sunflower breeding program aimed to produce F1 hybrid with high seed and oil yield. In this study, two sunflower inbred lines and their hybrid were used to investigate molecular mechanism of heterosis through proteomic approach. A completely randomized design was conducted to study the 13 morphological characters. Two-dimensional electrophoresis along with CBB staining was applied for hybrid and its parental lines. Comparison of hybrid with parental lines discovered a total of 153 proteins spots, of which 14 spots indicated non-additive changes. Comparing hybrid with parental lines B330 indicated 176 protein spots, of which 17 spots showed significant changes in hybrid. Comparison of two-dimensioned gels between hybrid and R46 line appeared 187 protein spots, of which 11 protein spots showed significant expression changes. Classification of up-regulated proteins in hybrid in metabolism and photosynthesis might be the molecular reason of hybrid vigor and growth compare with the parent. Other changed proteins were grouped in energy, cell detoxification, signal transduction and defense/disease.