Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | September 1, 2015

| Download 1

Himalrandia tetrasperma, ethanolic extracts preliminary phytochemical analysis, antibacterial and antifungal activities

Syed M. Salman, Sher Bahadar Khan, Shaukat Ali, Durr e Shahwar, M. Siddique Afridi, Zahid Khan, Ghausia Lutfullah

Key Words:

J. Bio. Env. Sci.7(3), 100-109, September 2015


JBES 2015 [Generate Certificate]


The current piece of research was designed to determine the anti-microbial potential of root, stem and leaves of selected medicinal plant Himalrandia tetrasperma against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi, (gram-negative) Staphylococcus aureus, Bacillis subtillis, Bacillis cereous (gram-positive) bacteria and Candida albicon by disc diffusion method. Ethanol extracts of root, stem and leaves of Himalrandia tetrasperma were applied in concentration of 1mg and 2mg/disc in 6µl and 12 µl volumes. Antibiotics Azithromycin, Ciprofloxacin and Chlotrimazole were applied 6 µl/disc separately as positive controls for gram positive and gram negative bacteria and Candida albicon. Dimethylsulfoxide (DMSO) was applied 6 µl/disc separately as negative controls. These plates were then incubated at 37co for 24 hours. The diameter of zone of inhibition was taken as a measure of antimicrobial effect of the extracts. All the parts of selected plants show sensitivity against selected microbial species. Phytochemical screening of the plants show that most of the active phytochemical, for which tests were carried out, were present in various parts of the plants. Analysis were carried out for the presence of alkaloids, tannins, anthraquinone, Saponins, terpenoids, flavonoids, phlobotannins, steroids, glycosides and reducing sugars, and the results were tabulated .The presence of phytoconstituents and antimicrobial activities shows that the selected plant could be used as a potential source of antimicrobial drugs.


Copyright © 2015
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Himalrandia tetrasperma, ethanolic extracts preliminary phytochemical analysis, antibacterial and antifungal activities

Adekunle AS, Adekunle OC. 2009. Preliminary antimicrobial properties of aqueous extract of plants infectious diseases. Biology and Medicines 1(3), 20-24.

Ahmed M, Aslam N, Khan RA. 2011-12. Antimicrobial activity of crude methanolic extract of Periploca aphylla. Journal of Medicinal Plants Research 5(32), 7017-7021. http://dx.doi.org/10.5897/JMPR11.1112.

Ahmad M, Khan MA, Qureshi RA. 2003. Ethnobotanical study of some cultivated plants of chuch region (District Attock). Hamdard Medicus. 46, 15-19.

Anwar N, Salik S, Ahmad D. 2009. Antibacterial Activity of Otostegia limbata. International Journal of Agriculture and Biology 11(5), 647-650.

Benoitvical F, Valentin A, Mallic M, Bassierc JM. 2001. Anti-plasmodial activity of Colchlospermum planchonii and C. tinctorium tubercle essential oils. Journal of Essential Oil Research 13(1), 65-67. http://dx.doi.org/10.1080/10412905.2001.9699609.

Bhat S, Mercy L, Chethan K, Sukesh KV, Chandrasheker KR. 2009. Anti-microbial spectrum and Phytochemical study of Hopea parviflora Biddom saw dust extracts. Journal of Phytology 1(6), 469-474.

Briskin DP. 2000. Medicinal plants and Phytomedicines Linking plant biochemistry and physiology to human health. Plant Physiology 124, 507-514. http://dx.doi.org/10.1104/pp.124.2.507

Edeoga HO, Okwu D, Mbaebie BO. 2005. Phytochemical constituents of some Nigerian Medicinal plants. African Journal of Biotechnology 4(7), 685-688. http://dx.doi.org/10.5897/AJB2005.000-312.

Eloff JN, Famakin JO, Katerere DRP. 2005. Combretum woodii (Combretaceae) leaf extracts have high activity against Gram- negative and Gram-positive bacteria. African journal of Biotechnology 4(10), 1161-1166.

Ferdous AJ, Islam SM, Ahsan M. hassan CM, Ahmad ZV. 1992. In vitro antibacterial activity of the volatile oil of Nigella sativa seeds against multiple drug-resistant isolates of Shigella spp. and isolates of Vibriocholerae  and  Escherichia  coli:  Phytotherapy Research 6(3), 137-140. http://dx.doi.org/10.1002/ptr.2650060307.

Geoffrey GC. 1996. Medicinal plants and the control of parasites. Transactions of Royal Society of Tropical Medicine Hygiene 90, 605-609. http://dx.doi.org/10.1016/S0035-9203(96)90404-6.

Hammer KA, Carson CF, Riley TV. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86(6), 985-990. http://dx.doi.org/10.1046/j.1365-2672.1999.00780.x.

Harborne JB. 1973. Phytochemical Methods 3rd Edn.Chapman and Hall Ltd., London, 135-203.

Hassawi D, Abeer K. 2006. Antimicrobial activity of medicinal plants against Candida albicans. Journal of Biological Sciences 6(1), 109-114.

Henrich M, Barnes J, Gibbons S, Williamson EM. 2012. Fundamentals of Pharmacognosy Phytotherapy, Churchill Livingstone, Edinburgh, Elsevier, Canada.

Hérouart D, Sangwan RS, Fliniaux MA, Sangwan-Norreel BS. 1988. Variations in the Leaf Alkaloid Content of Androgenic Diploid Plants of Datura anoxia. Planta Med 54, 14-17. http://dx.doi.org/10.1055/s-2006-962320

Jehan B, Huma A, Mubarak AK, Ayyub K, Muhammad S, Mohammad S, Amjad I, Mohammad T. 2011. Antimicrobial activities of different solvents extracted samples of Linum usitatissimum by disc diffusion method. African Journal of Biotechnology 10(85), 19825. http://dx.doi.org/10.5897/AJB11.229.

Kambu K, Di Phenzu N, Coune C, Wauter JN, Angenot L. 1982. Plants Medicine ET Phytotherapie, 34.

Kappers IF, Aharoni A, Van Herpen TW, Luckerhoff LL, Dicke M. 2005. Genetic engineering of terpenoids metabolism attracts bodyguards to Arabidopsis. Science 309, 2070-2072. http://dx.doi.org/10.1126/science.1116232.

Khan RA, Khan MR, Sahreen S. 2012. Protective effect of Sonchus asper extracts against experimentally-induced lung injuries in rats: A novel study. Experimental and Toxicologic Pathology 64 (7-8), 725-731. http://dx.doi.org/10.1016/j.etp.2011.01.007

Krishnaiah D, Sarbatly R, Bono A. 2007. Phytochemical antioxidants for health and medicine – A move towards nature. Biotechnology Molecular Biology Review 2(4), 097-104.

Krishnaiah D, Devi T, Bono A, Sarbatly R. 2009. Studies on Phytochemical Constituents of Six Malaysian Medicinal Plants. Journal of Medicinal Plants Research 3, 67.

Lemos TLG, Matos FJA, Alencar JW, Crareiro AA, Clark AM, Chesnary JD. 1990. Antimicrobial activity of essential oils of Brazilian plants. Phytotherapy Research 4(2), 82-84. http://dx.doi.org/10.1002/ptr.2650040210

Lucy H, Edgar JD. 1999. Medicinal plants: a re-emerging health aid. Electronic Journal of Biotechnology 2, 56-70. http://dx.doi.org/10.2225/vol2-issue2-fulltext-2.

Newman DJ, Cragg GM, Snader KM. 2002. The influence of natural products upon drug discovery. Natural Products Reports 17, 215- 234. http://dx.doi.org/10.1039/A902202C.

Ríos JL,  Recio M C. 1982. Medicinal plants and antimicrobial activity. Journal of Ethanopharmacology 100 (1-2), 80-84. http://dx.doi.org/10.1016/j.jep.2005.04.025

Santos FA, Rao VSN, Silveria ER. 1998. Investigations on the antinociceptive effect of Psidium guajava leaf essential oil and its major constituents. Phytotherapy Research 12(1), 24-27. http://dx.doi.org/10.1002/(SICI)10991573(19980201 )12:1<24::AID-PTR181>3.0.CO;2-B.

Trease GE, Evans WC. 1978. Pharmacognosy. 11th Edn. Brailliar Tiridel and Macmillian Publishers, London.

Vardar-unlu G, Cadan F, Sokmen A, Deferera Polissiou M, Sokmen M, Donmez E, Tap Bektas. 2003. Antimicrobial and Antioxidant Activity of the Essential Oil and Methanol Extracts of Thymus pectinatus Fisch. et Mey. Var. pectinatus (Lamiaceae). Journal of Agriculture Food Chemistry 51(1), 63-67. http://dx.doi.org/10.1021/jf025753e.

Yildrim A, Mavi A, Oktay M, Kara AA, Aligur OF, Bilaloglu V. 2000. Comparison of Antioxidant and Antimicrobial activities of Tilla argenta, Salvia trilaba, and Camilla sinensis extracts. Journal of Agriculture and Food Chemistry 48(10), 5030-5034. http://dx.doi.org/10.1021/jf000590k.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background