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Himalrandia tetrasperma, ethanolic extracts preliminary phytochemical analysis, antibacterial and antifungal activities

Research Paper | September 1, 2015

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Syed M. Salman, Sher Bahadar Khan, Shaukat Ali, Durr e Shahwar, M. Siddique Afridi, Zahid Khan, Ghausia Lutfullah

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J. Bio. Env. Sci.7( 3), 100-109, September 2015


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The current piece of research was designed to determine the anti-microbial potential of root, stem and leaves of selected medicinal plant Himalrandia tetrasperma against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi, (gram-negative) Staphylococcus aureus, Bacillis subtillis, Bacillis cereous (gram-positive) bacteria and Candida albicon by disc diffusion method. Ethanol extracts of root, stem and leaves of Himalrandia tetrasperma were applied in concentration of 1mg and 2mg/disc in 6µl and 12 µl volumes. Antibiotics Azithromycin, Ciprofloxacin and Chlotrimazole were applied 6 µl/disc separately as positive controls for gram positive and gram negative bacteria and Candida albicon. Dimethylsulfoxide (DMSO) was applied 6 µl/disc separately as negative controls. These plates were then incubated at 37co for 24 hours. The diameter of zone of inhibition was taken as a measure of antimicrobial effect of the extracts. All the parts of selected plants show sensitivity against selected microbial species. Phytochemical screening of the plants show that most of the active phytochemical, for which tests were carried out, were present in various parts of the plants. Analysis were carried out for the presence of alkaloids, tannins, anthraquinone, Saponins, terpenoids, flavonoids, phlobotannins, steroids, glycosides and reducing sugars, and the results were tabulated .The presence of phytoconstituents and antimicrobial activities shows that the selected plant could be used as a potential source of antimicrobial drugs.


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