Histological assessment of topical mixture of Thymus vulgaris honey and Nigella sativa L seed powder on skin wound healing in sheep
Paper Details
Histological assessment of topical mixture of Thymus vulgaris honey and Nigella sativa L seed powder on skin wound healing in sheep
Abstract
Honey and Nigella sativa L has been known for millennia of years for its therapeutic healing properties. This present histopathological study aims to evaluate the healing activity of Algerian bee honey derived from the nectar of Thymus vulgaris mixed with N. sativa seed powder on a sheep model. Induction of wounds with longitudinal incisions of full thickness, was carried out in the dorsal part of 09 healthy sheep belonging to the animal facility of the veterinary department of the University of Batna 1. After inductions of the wounds, the animals were distributed to the randomly in 03 batches of animals of 03 sheep for each with appropriate topical care for each batch: the batch of animals treated with Thymus vulgaris honey mixed with N. sativa seed powder (HNS), the Madicassol batch and the Vaseline. The topical application of the various treatments was carried out once a day for 02 successive weeks. Histopathological evaluation was performed by measuring scar tissue thickness. In the batch of animals treated with HNS mixture, the thickness of the scar tissues revealed a significant decrease compared to the Madicassol and Vaseline batches. No keloid or detectable in the wounds of the mixture HNS lot. The results of this experimental study suggested that the topical use of local honey from the nectar of T.vulgaris mixed with N. sativa seed powder significantly reduced the thickness of wound healing in sheep.
Abuharfeil N, Al-Oran R, Abo-Shehada M. 1999. Effect of bee honey on the proliferative activity of human B- and T-Lymphocytes and the activity of phagocytes. Food and Agricultural Immunology 11, 169-177. https://dx.doi.org/10.1080/09540109999843.
Alishahi KM, Mofidpoor H, Alishahi MAK. 2014. Histopathological evaluation of the effect of platelet-rich fibrin on canine cutaneous incisional wound healing. World Applied Sciences Journal 31, 676-680.
Alster TS, Tanzi EL. 2003. Cicatrices hypertrophiques et chéloïdes : étiologie et prise en charge. American Journal of Clinical Dermatology 4,235-243.
AL-Waili N, Al-Ghamdi A, Javed Ansari M, Al-Attal Y, Salom K. 2012 .Synergistic Effects of Honey and Propolis toward Drug Multi-Resistant Staphylococcus Aureus, Escherichia Coli and Candida Albicans Isolates in Single and Polymicrobial Cultures. International Journal of Medical Sciences 9, 793-800. https://dx.doi.org/10.7150/ijms.4722.
Boyce ST, Lalley AL. 2018. Tissue engineering of skin and regenerative medicine for wound care. Burns & Trauma 6, 4. https://dx.doi.org/10.1186/s41038-017-0103-y.
Chicharro-Alcántara D, Rubio-Zaragoza M, Damiá- Giménez E, Carrillo-Poveda JM, Cuervo-Serrato B, Peláez-Gorrea P, Sopena-Juncosa JJ. 2018. Platelet-rich plasma: New insights for cutaneous wound healing management. Journal of Functional Biomaterials 9, 1-20. https://dx.doi.org/10.3390/jfb901001010.
Daikh B, Deffa O. 2021. Comparative study of the therapeutic efficacy of autologous platelet-rich plasma and honey in healing skin wounds in sheep. Veterinary World 14, 2170-2177. https://dx.doi.org/10.14202/vetworld.2021.2170-2177.
Forouzanfar F, Bazzaz BS, Hosseinzadeh H. 2014. Black cumin (Nigella sativa) and its constituent: a review on antimicrobial effects. Iranian Journal of Basic Medical Sciences 17, 929-38.
Gentile P , Calabrese C, De Angelis B, Dionisi L, Pizzicannella J, Kothari A, De Fazio D, Garcovich S. 2020. Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation. International Journal of Molecular Sciences 2, 431. https://dx.doi.org/10.3390/ijms21020431.
Ghorbanibirgani A, Khalili A, Rokhafrooz D. 2014. Comparing Nigella sativa oil and fish oil in treatment of vitiligo. Iranian Red Crescent Medical 16, e4515. https://dx.doi.org/ 10.5812/ircmj.4515.
Heath M, Jaimes Na, Lemos B, Mostaghimi A, C Wang L, JDdPablo FP, Nghiem P. 2008. Clinical characteristics of Merkel cell carcinoma at diagnosis in 195 patients: the AEIOU features. The Journal of the American Academy of Dermatology 58, 375-381. https://dx.doi.org/10.1016/j.jaad.2007.11.020.
Jawad AH, Azhar IY, Khalil IH. 2014. Evaluation of efficacy safety and antioxidant effect of Nigella sativa in patients with psoriasis: a randomized clinical trial. Clinical and Experimental Investigations 5, 188–193. https://dx.doi.org/ 10.5799/ahinjs.01.2014.02.0387.
Li J, Chen J, Kirsner RS. 2007. Pathophysiology of acute wound healing. Clin Dermato 25, 9-18. https://dx.doi.org/10.1016/j.clindermatol.2006.09.007.
Limandjaja GC, Niessen FB, Scheper RJ, Gibbs S. 2020. The Keloid Disorder: Heterogeneity, Histopathology, Mechanisms and Models. Frontiers in Cell and Developmental Biology 8, 360.
McLoone P, Tabys D, Fyfe, L. 2020 .Honey Combination Therapies for Skin and Wound Infections: A Systematic Review of the Literature. Clinical, Cosmetic and Investigational Dermatology 13, 875–888.
Niessen FB, Spauwen PHM, Schalkwijk JK, Moshe MD. 1999. On the Nature of Hypertrophic Scars and Keloids: A Review. Plastic and Reconstructive Surgery 104, 1435–1458. https://dx.doi.org/10.1097/00006534-199910000-00031.
O’Shaughnessy KD, De La Garza M, Roy NK, Mustoe TA. 2009. Homeostasis of the epidermal barrier layé: A theory of how occlusion reduces hypertrophic scarring. Wound Repair and Regeneration 17, 700–708. https://dx.doi.org/ 10.1177/1721727X1401200307.
Pizzicannella J, Gugliandolo A, Orsini T, Fontana A, Ventrella A, Mazzon E, Bramanti P, Diomede F, Trubiani O. 2019. Engineered extracellular vesicles from human periodontal-ligament stem cells increase VEGF/ VEGFR2 expression during bone regeneration. Frontiers in Physiology 10, 512. https://dx.doi.org/ 10.3389/fphys.2019.00512.
Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. 2019. Wound Healing: A Cellular Perspective. Physiological Reviews 99, 665–706. https://dx.doi.org/ 10.1152/physrev.00067.2017.
Sabine AE, Paul M, Tomic-Canic M. 2014. Wound repair and regeneration: Mechanisms, signaling, and translation. Science Translational Medicine 6, 265sr6. https://dx.doi.org/10.1126/scitranslmed.3009337
Salman H, Kashmoola MA, Al-Waiz MM, Al-Sandooq TA. 2008. Differences between low level laser therapy and triamcinolone acetonide kenalog on healing of recurrent aphthous ulceration. Annals of the College of Medicine Mosul 34, 35–41.
Santuzzi CH, Buss HF, Pedrosa DF, Freire MO, Nogueira BV, Gonçalves WL. 2011. Combined use of low level laser therapy and cyclooxygenase-2 selective inhibition on skin incisional wound reepithelialization in mice: a preclinical study. An Bras Dermatol 86, 278–283. https://dx.doi.org/10.1590/s0365-05962011000200011.
Takzare N, Hassanzadeh G, Rouini MR, Manayi A, Hadjiakhondi A, Zolbin MM. 2017. Evaluation of the effects of local application of thyme honey in open cutaneous wound healing. Iranian Journal of Public Health 46, 545-551.
Uwatosin OM, Olabanji JK, Oluwatosin OA, Tijani LA, Onyechi HU. 2000. A comparison of topical honey and phenytoin in the treatment of chronic leg ulcers. African Journal of Medicine and Medical Sciences 29, 31–34.
Veith AP, Henderson K, Spencer A, Sligar AD, Bake AB. 2020. Therapeutic Strategies for Enhancing Angiogenesis in Wound Healing. Advanced Drug Delivery Reviews 146, 97–125. https://dx.doi.org/10.1016/j.addr.2018.09.010.
Won JK, Young CN, Bum SS, Hyeon AK, Woo HH, Gum HA, Choi HC, Seo UL. 2013. The effects of topical agent (Kelo-Cote or contractubex) massage on the thickness of post-burn scar tissue formed in rats. Archives of Plastic Surgery 40, 697-704.
Yousefi M, Barikbin B, Kamalinejad M, Abolhasani E, Ebadi A, Younespour S. 2013. Comparison of therapeutic effect of topical Nigella with Betamethasone and Eucerin in hand eczema. Journal of the European Academy of Dermatology and Venereology 27, 1498–1504. https://dx.doi.org/10.1111/jdv.12033.
Deffa Ouafa, Daikh Badis (2022), Histological assessment of topical mixture of Thymus vulgaris honey and Nigella sativa L seed powder on skin wound healing in sheep; IJB, V21, N1, July, P200-208
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