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Histopathological changes on testes of guppy fish Poecilia reticulate Peters (1859) exposed to vydate

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Research Paper 01/05/2018
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Histopathological changes on testes of guppy fish Poecilia reticulate Peters (1859) exposed to vydate

Nor Al-Huda R. Mayea, Mukhtar K. Haba
J. Bio. Env. Sci.12( 5), 63-69, May 2018.
Certificate: JBES 2018 [Generate Certificate]
Key: Guppy fishPesticidePoecilia reticulataTestesVydate

Abstract

The present study was aimed to identify the histopathological changes of the testicle in the Guppy fish resulting from exposure to the vydate pesticide; the investigation conducted on 60 fish of Guppy males collected from local markets, fish were divided into 4 groups, each one included 15 fish, group 1 has exposed for (0.3 mg/L) of vydatepesticide, group 2 Exposed for (0.5mg/L), group 3 exposed for (0.8 mg/L), and total control. Then the groups were sacrificed at (2, 4 & 6) weeks and the samples of testes were collected and fixed with Bouin’s solution  and processed with routine paraffin technique and tissue sections were stained with H&E stain. The results showed that the testes of the control group were surrounded by a connective tissue capsule which contained melanocytes. The testicular parenchyma composed of groups of seminiferous tubules which separated from each other by interstitial tissue which contains Lyedic cells. The germinal epithelium showed variable stages of spermatogenesis started with primordium germ cells with divided to give rise spermatogonia, primary spermatocytes, spermatid and spermatozoa. In treated groups, at two weeks exposure tovydate showed mild vascular congestion of the interstitial tissue, blood vessels and shrinkage of the Lyedic cells; while at four weeks exposure the signs revealed advanced changes within seminiferous tubules showed degenerative changes and necrosis and there were appearance of giant cells a among developing germ cells. The study concluded that the changes are increased with increasing both the concentration and the duration of exposure, as evidenced by our current study.

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