Welcome to International Network for Natural Sciences | INNSpub

Histopathology of liver and kidneys for the assessment of genotoxicity in Indian major carps treated with domestic waste and industrial effluents of Chakbandi drain water

Research Paper | December 1, 2017

| Download 9

Muhammad Arshad Rana, Tayyaba Sultana, Salma Sultana, Bilal Hussain

Key Words:

Int. J. Biosci.11( 6), 178-185, December 2017

DOI: http://dx.doi.org/10.12692/ijb/11.6.178-185


IJB 2017 [Generate Certificate]


This project was planned to study the exposure effects of domestic waste and industrial effluents on water quality parameters and fish under laboratory conditions. Faisalabad the so called “Manchester” of Pakistan has plastic, leather, textile, dying, printing, finishing, seizing industries, sugar mills and a large number of factories that release the variety of industrial discharges and municipal wastes into River Chenab through Chakbandi drain. In this study, Chakbandi drain’s water was collected from the selected sites in the month of April, May and June, 2016 and applied to fingerling’s of three Indian major carps i.e. Catla catla, Labeo rohita and Cirrhinus mrigala under laboratory conditions in glass aquaria. After determining the LC50, the sub-lethal dilutions i.e. 20%, 25%, 30%, 35% and 40% of drain water were tested for three month’s acute toxicity trial. After three months, fish tissues i.e. liver and kidneys were taken by dissecting the control and experimental fishes for histopathological studies. Histopathological analysis of control fish revealed the normal structures whereas treated fish showed different abnormalities with respect to liver and kidney tissues. Conclusively, histopathological biomarkers approach was found to be reliable for the assessment of environmental pollution. Moreover, findings of this study are helpful for the evaluation of pollution levels reaching in aquatic fauna particularly fish and indirectly to human populations.


Copyright © 2017
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Histopathology of liver and kidneys for the assessment of genotoxicity in Indian major carps treated with domestic waste and industrial effluents of Chakbandi drain water

Adeyemo OK. 2005. Haematological and histopathological effects of Cassava Mill Effluent in Clarias gariepinu. African Journal of Biomedical Research 8, 179 -183.

Ali FK, El-Shehawi AM, Sheehy MA. 2008. Micronucleus test in fish genome: sensitive monitor for aquatic pollution. African Journal of Biotechnology 7(5), 606- 612.

Anim AK, Ahialey EK, Duodu GO, Ackah M, Bentil NO. 2011. Accumulation profile of heavy metals in fish samples from Nsawam, along the Densu River, Ghana. Research Journal of Environment and Earth Sciences 3(1), 56-60.

El-Bassir AHA, Ashjgan MA, Maab EE, Ryan SG, Alim DIM. 2017. Levels of four heavy metals in two fish species collected from aldubaseen area in khartoum state, sudan. EC Nutrition 6(4), 130-134.

Faheem M, Jahan N, Lone KP. 2016. Histopathological effects of bisphenol on liver, kidneys and gills of Indian major carp, Catla catla (Hamilton, 822). Journal of Animal and Plant Sciences 26(2), 514-522.

Fernandes C, Fontainhas A, Peixoto F, Salgado MA. 2007. Bioaccumulation of heavy metals in Liza saliens from the Esmoriz Paramos Coastal lagoon, Portugal. Ecotoxicology and Environmental Safety 66, 426-431.

Gupta V, Malik DS, Dinesh K. 2017. Risk assessment of heavy water pollution in middle stretch of river Ganga: An introspection. International Research Journal of Environment Science 6(2), 62-71.

Hayat S, Javed M, Razzaq S. 2007. Growth performance of metal stressed major carps viz. Catla catla, Labeo rohita and Cirrhinus mrigala reared under semi-intensive culture system. Pakistan Veterinary Journal 27(1), 8-12.

Jamdade AB, Gawande SM. 2017. Analysis of water quality parameters. International Journal of Engineering Research 6(3), 145-148.

Javed M. 2005. Growth responses of Catla catla, Labeo rohita and Cirrhina mrigala for bio-accumulation of zinc during chronic exposure. Pakistan Journal of Biological Science 8, 1357-1360.

Junianto, Zahidah & Apriliani IM. 2017. Evaluation of heavy metal contamination in various fish meat from Cirata Dam, West Java, Indonesia. AACL Bioflux 10(2), 711-717.

Latif A, Ali M, Kaoser R, Iqbal R, Umer K, Latif M, Qadir S, Iqbal F. 2012. Effect of cadmium chloride and ascorbic acid exposure on the vital organs of freshwater Cyprinid, Labeo rohita. African Journal of Biotechnology 11(33), 8398-8403.

Liebel S, Tomotake MEM, Oliveira RCA. 2013. Fish histopathology as biomarker to evaluate water quality. Ecotoxicology and Environmental Containination 8(2), 9-15.

Mir MI, Khan S, Bhat SA, Reshi AA, Shah FA, Balki MH, Manzoor R. 2014. Scenario of genotoxicity in fishes and its impact on fish industry. Journal of Environmental Science, Toxicology and Food Technology 8(6), 65-76.

Paul R, Guite LL, Ramanujam SN. 2014. Copper and Cadmium induced histopathological alterations in liver of Heteropneustes fossilis (Bloch) at varying water pH. International Journal of Fisheries and Aquatic Studies 1(5), 38-42.

Reddy PB, Rawat SS. 2013. Assessment of aquatic pollution using histopathology in fish as a protocol. International Research Journal of Environmental Sciences 2(8), 79- 82.

Ruane NM, Bonga WSE, Balm PHM. 1999. Differences between rainbow trout and brown trout in the regulation of the pituitary inter-renal axis and physiological performance during confinement. Comparative Endocrinology, 115, 210-219.

Sabullah MK, Ahmad SA, Shukor MY, Gansau AJ, Syed MA, Sulaiman MR, Shamaan NA. 2015. Heavy metal biomarker: Fish behavior, cellular alteration, enzymatic reaction and proteomics approaches. International Food Research Journal 22(2), 435-454.

Shaheen T, Jabeen F. 2015. Effect of various doses of Cr (VI) on survival and growth of Cyprinus carpio. Pakistan Journal of Zoology 47(4), 913-919.

Sultana T, Siddique A, Sultana S, Mahboob S, Al-Ghanim K, Ahmed Z. 2016. Fish scales as a non-lethal tool of the toxicity of wastewater from the River Chenab. Environment Science and Pollution Research 24(3), 2464-2475.

Thangam Y. 2014. Histopathological studies on nitrite toxicity to freshwater fish, Cirrhinus mrigala. Journal of Environmental Science, Toxicology and Food Technology 8(4), 10-14.

Viana AP, Fredou FL, Montes CS, Rocha RM. 2013. Fish histopathology and catalase activity as biomarkers of the environmental quality of the industrial district on the Amazon estuary, Brazil. Acta Scientiarum. Biological Sciences Maringa 35(3), 395-401.

WHO. 2013. Guidelines for drinking water quality, WHO Water Quality and Health Strategy 2013-2020. Retrieved from NIMH Web site via GPO Access: www.who.int/water_sanitation_health/dwq/en/

Zaqoot HA, Aish AM, Wafi HN. 2017. Baseline concentration of heavy metals in fish collected from Gaza fishing harbor in the Mediterranean Sea along Gaza Coast, Palestine. Turkish Journal of Fisheries and Aquatic Sciences 17, 101-109.