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Host plants induced changes in the digestive enzymes activities and growth parameters of Eri silkworm (Samia ricini) Larvae

Hatarkhi Mwchahary, Dulur Brahma

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Int. J. Biosci.22(2), 215-223, February 2023

DOI: http://dx.doi.org/10.12692/ijb/22.2.215-223


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In leaf feeding insects, proper nourishment of larvae is dependent on the balance between nutritional content of food plants and the action of digestive enzymes in the digestive tract. In present investigation, gut digestive enzyme activities (α-amylase, proteinase and lipase) and their influence on the larval growth parameters of Eri silkworm (Samia ricini) larvae reared on different food plants viz. castor (Ricinus communis), tapioca (Manihot esculenta) and papaya (Carica papaya) were assessed. The larvae on castor showed highest α-amylase (1.50 ± 0.045 U/ml) and lipase (0.61 ± 0.012 U/ml) activities followed by larvae on tapioca (1.15 ±0.007 U/ml and 0.53 ± 0.059 U/ml respectively) and the lowest in papaya fed larvae (1.04 ± 0.009 U/ml and 0.39 ± 0.011 U/ml respectively). The highest proteinase activity was recorded in papaya fed larvae (0.045 ± 0.004 U/ml) and the lowest in castor (0.033 ± 0.001 U/ml). Our results showed highest larval survivability in castor (94.56 ± 1.84%) followed by tapioca (85.56 ± 1.93%) and lowest in papaya (62.22 ± 1.92%). Furthermore, the larvae fed on castor had the highest larval weight (6.92 ± 0.59 g) and silk gland weight (1.42 ± 0.07 g) whereas the lowest values of larval weight and silk gland weight were recorded in larvae reared on papaya leaves (4.33 ± 0.61 g and 0.65 ± 0.14 g respectively). Above results indicated that enzyme activities are functionally related with the type of food plants which subsequently determines the nutritional responses of the silkworm fed on different food plants.


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Host plants induced changes in the digestive enzymes activities and growth parameters of Eri silkworm (Samia ricini) Larvae

Ahmadi F, Khani A, Ghadamyari M. 2012. Some properties of α-amylase in the digestive system and head glands of Cryptolaemus montrouzieri (Coleoptera: Coccinellidae). Journal of Crop Protection 1(2), 97-105.

Bernfeld P. 1955. Amylase, α and β. Methods in enzymology, 1, 149-158. http://dx.doi.org/10.1016/00

Birari VV, Siddhapara MR, Desai AV. 2019. Rearing performance of eri silkworm, Samia ricini (Dovovan) on different host plants. Journal of Farm Sciences 32(4), 443-446.

Borah SD, Saikia M, Boro P. 2020. Rearing performance of two selected eco-races of eri silkworm (Samia ricini Donovan) fed with castor and borpat leaves during spring and autumn season in Assam. Journal of Entomology and Zoology Studies 8(3), 2024-2028.

Chakrabarty S, Saha AK, Manna B, Bindroo BB. 2012. Light and Electron Microscopy of Nosema ricini (Microsporidia: Nosematidae), The causal pathogen of Pebrine disease in eri silkworm: Life cycle and cross-infectivity. Applied Biological Research 14(1), 1-14.

Das SK, Sahu BK, Singh D. 2020. Host plant diversity of non-mulberry silkworms: A review. Journal of Pharmacognosy and Phytochemistry 9(3), 109-113.

Deuri J, Barua PK, Sarmahmc, Ahmed SA. 2017. Biochemical attributes of castor and tapioca leaves, the promising food plants of eri silkworm (Samia ricini Donovan). International Journal of Ecology and Ecosolution 4(1), 1-4. https://doi.org/10.30918/ijee.41.17.012

Gangwar SK. 2010. Impact of varietal feeding of eight Mulberry varieties on Bombyx mori L. Agriculture and Biology Journal of North America 1(3), 350-354.

Gururaj, Kumar KP, Naika R. 2017. Rearing performance of eri silkworm, Samia cynthia ricini (Boiduval) (Lepidoptera: Saturniidae) on cultivars of Castor. Journal of Entomology and Zoology Studies 5(5), 816-821.

Hemati SA, Naseri B, Ganbalani GN, Dastjerdi HR, Golizadeh A. 2012. Digestive proteolytic and amylolytic activities and feeding responses of Helicoverpa armigera (Lepidoptera: Noctuidae) on different host plants. Journal of Economic Entomology 105(4), 1439-1446.

Holtof M, Lenaerts C, Cullen D, Vanden Broeck J. 2019. Extracellular nutrient digestion and absorption in the insect gut. Cell and Tissue Research 377, 397-414.

Kannan M, Suganya T, Arunprasanna V, Rameshkumar N, Krishnan M. 2015. An efficient method for extraction of genomic DNA from insect gut bacteria-culture dependent. Current Research in Microbiology and Biotechnology 3(1), 550-556.

Konno K, Hirayama C, Nakamura M, Tateishi K, Tamura Y, Hattori M, Kohno K. 2004. Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex. The Plant Journal 37(3), 370-378.

Kotkar HM, Sarate PJ, Tamhane VA, Gupta VS, Giri AP. 2009. Responses of midgut amylases of Helicoverpa armigera to feeding on various host plants. Journal of Insect Physiology 55(8), 663-670.

Kumar R, Elangovan V. 2010. Assessment of the volumetric attributes of eri silkworm (Philosamia ricini) reared on different hosts plants. International Journal of science and nature 1(2), 156-160.

Kumar R, Gangwar SK. 2010. Impact of varietal feeding on Samia ricini Donovan in spring and autumn season of Uttar Pradesh. ARPN Journal of Agricultural and Biological Science 5(3), 46-51.

Mala N, Vijila K. 2017. Changes in the activity of digestive enzymes produced from the gut microflora of silkworm Bombyx mori L. (Lepidoptera: Bombycidae) in response to fortification of mulberry leaves. International Journal of Current Microbiology and Applied Sciences 6(11), 225-236.

Manjula S, Sabhanayakam S, Mathivanan V, Saravanan N. 2010. Studies on the changes in the activities of digestive enzymes in the midgut of silkworm Bombyx mori (L). (Lepidoptera: Bombycidae) fed with mulberry leaves supplemented with Indian bean (Dolichos lablab). International Journal of Biological and Medical Research 1(4), 168-171.

Mansouri SM, Ganbalani GN, Fathi SSA, Naseri B, Razmjou J. 2013. Nutritional indices and midgut enzymatic activity of Phthorimaea operculella (Lepidoptera: Gelechiidae) larvae fed different potato germplasms. Journal of Economic Entomology 106(2), 1018-1024.

Mardani-Talaee M, Rahimi V, Zibaee A. 2014. Effects of host plants on digestive enzymatic activities and some components involved in intermediary metabolism of Chrysodeixis chalcites (Lepidoptera: Noctuidae). Journal of Entomological and Acarological Research 46(3), 96-101. https://doi.org /10.4081/jear.2014.3224

Massadeh MI, Sabra FM. 2011. Production and characterization of lipase from Bacillus stearothermophilus. African Journal of Biotechnology 10(61), 13139-13146.

Mendiola-Olaya E, Valencia-Jimenez A, Valdes-Rodrıguez S, Delano-Frier J, Blanco-Labra A. 2000. Digestive amylase from the larger grain borer, Prostephanus truncatus Horn. Comparative Biochemistry and Physiology, Part B 126(3), 425-433. https://doi.org/10.1016/s0305-0491(00)00216-9

Namin FR, Naseri B, Razmjou J. 2014. Nutritional performance and activity of some digestive enzymes of the cotton bollworm, Helicoverpa armigera, in response to seven tested bean cultivars. Journal of Insect Science 14(93), 1-18. https://doi.org/10.1673/031.014.93

Naseri B, Kouhi D, Razmjou J, Golizadeh A. 2014. Digestive enzymatic activity and nutritional responses of Helicoverpa armigera (Lepidoptera: Noctuidae) larvae fed various tomato cultivars. Journal of economic entomology 107(4), 1655-1661. https://doi.org/10.1603/ec13284

Oftadeh M, Sendi JJ, Zibaee A, Valizadeh B. 2014. Effect of four varieties of mulberry on biochemistry and nutritional physiology of mulberry pyralid, Glyphodes pyloalis Walker (Lepidoptera: Pyralidae). Journal of Entomological and Acarological Research 46(2), 42-49. https://doi.org/10.4081/jear

Oyebanji O, Soyelu O, Bamigbade A, Okonji R. 2014. Distribution of digestive enzymes in the gut of American cockroach, Periplaneta americana (L.). International Journal of Scientific and Research Publications 4(1), 1-5.

Pauchet Y, Muck A, Svatos A, Heckel DG, Preiss S. 2008. Mapping the larval midgut lumen proteome of Helicoverpa armigera, a generalist herbivorous insect. Journal of Proteome Research 7(4), 1629-1639. https://doi.org/10.1021/pr7006208

Pechan T, Cohen A, Williams WP, Luthe DS. 2002. Insect feeding mobilizes a unique plant defense protease that disrupts the peritrophic matrix of caterpillars. Proceedings of the National Academy of Sciences 99(20), 13319-13323. https://doi.org/ 10.1073/pnas.202224899

Rajadurai S, Tomy P, Shekhar MA. 2010. Seasonal rearing performance of eri silkworm, Samia cynthia ricini (Boisduval) on castor and tapioca under south Karnataka conditions. Indian Journal of Sericulture 49(2), 134-137.

Reddy DNR, Gowda M, Narayanaswamy KC. 2002. Ericulture: an insight. Zen Publication, Banglore, India 82.

Santana CC, Barbosa LA, Júnior IDB, Nascimento TGD, Dornelas CB, Grillo LAM. 2017. Lipase activity in the larval midgut of Rhynchophorus palmarum: biochemical characterization and the effects of reducing agents. Insects 8(3), 100. https://doi.org/10.3390/ insects8030100

Sarate PJ, Tamhane VA, Kotkar HM, Ratnakaran N, Susan N, Gupta VS, Giri AP. 2012. Developmental and digestive flexibilities in the midgut of a polyphagous pest, the cotton bollworm, Helicoverpa armigera. Journal of Insect Science 12(42), 1-16. https://doi.org/10.1673/031.012.4201

Suzuki T, Iwami M. 2021. Sequential changes in the regulatory mechanism of carbohydrate digestion in larvae of the silkworm, Bombyx mori. Journal of Comparative Physiology B 191(3), 439-453. https://doi.org/10.1007/s00360-021-01350-4

Suzuki T, Sakurai S, Iwami M. 2011. Steroidal regulation of hydrolyzing activity of the dietary carbohydrates in the silkworm, Bombyx mori. Journal of insect physiology 57(9), 1282-1289. https://doi.org/10.1016/j.jinsphys.2011.06.003

Ugo NJ, Ade AR, Joy AT. 2019. Nutrient composition of Carica papaya leaves extracts. Journal of Food Science and Nutrition Research 2(3), 274-282. https://doi.org/10.26502/ jfsnr.2642-11000026

Winkler UK, Stuckmann M. 1979. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. Journal of bacteriology 138(3), 663-670. https://doi.org/10.1128/jb.138.3.663-670.1979


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