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Hs-CRP and TNF- alpha in response to a stepwise incremental bicycle test in adult obese men

Research Paper | June 1, 2011

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Eizadi Mojtaba, Kohandel Mahdi, kasbparast JR Mehdi, Sarshin Amir

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J. Bio. Env. Sci.1( 3), 35-43, June 2011


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To compare of circulating levels of as tumor necrosis factor-α (TNF-α) and C reactive protein (CRP) as pro- inflammation cytokines between obese and none obese men and also to evaluate its response to a stepwise incremental bicycle test in obese men, eighty sedentary adult obese men (30 ≤ BMI ≤ 36, 40 ± 5 aged) and twelve none-obese men matched for age participated in this study. Fasting blood samples were obtained of two groups in order to measuring serum CRP, TNF-α, insulin and glucose. Depending of the values of insulin and glucose insulin resistance index (HOMA2) was calculated. Blood samples were also repeated in obese group after exercise test in order to determine all variables responses to exercise. Statistical analysis was performed with the SPSS software version 15.0 using an independent paired t-test. At baseline, Serum TNF-α, CRP, insulin and glucose concentrations and insulin resistance were significantly higher in obese group than in normal weight subjects (p ˂ 0.05). Compared to pre-training, the CRP and TNF-α levels increased significantly (P<0.01) after acute exercise while glucose concentration decreased significantly in studied obese participants (p<0.05). No significant differences were found in insulin resistance by cycling exercise with compared to baseline (p = 0.154). This study suggests obesity is associated with systemic inflammation and a incremental cycle test have inflammatory properties regardless that is not related with glucose concentration.


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Hs-CRP and TNF- alpha in response to a stepwise incremental bicycle test in adult obese men

Amina H, Abdul G, Abdul K, Jasim M. 2010. Association Between C Reactive Protein and Asthma. TurkishTorax Dergisi 11(3), 098-104.

Bal Y, Adas M, Helvaci A. 2010. Evaluation of the relationship between insulin resistance and plasma tumor necrosis factor-alpha, interleukin-6 and C-reactive protein levels in obese women. Bratisl Lek Listy 111(4), 200-4.

Campbell KL, Campbell PT, Urich CM, Wener M, Alfano CM, Foster-Schubert K. 2008. No reduction in C-reactive protein following a 12-month randomized controlled trial of exercise in men and women. Cancer Epidemiol Biomarkers Prev 17, 1714– 8.

Dandona  P,  Weinstock  R,  Thusu  K.  1998. Tumor necrosis factor-alpha in sera of obese patients: fall with weight loss. J Clin Endocrinol Metab 83, 2907-10.

de Salles BF, Simão R, Fleck SJ, Dias I, Kraemer-Aguiar LG, Bouskela E. 2010. Effects of resistance training on cytokines. Int J Sports Med 31(7), 441-50.

Gielen S, Adams V, Mobius-Winkler S, Linke A, Erbs S, Yu J et al. 2003. Anti-inflammatory effects of exercise training in the skeletal muscle of patients with chronic heart failure. J Am Coll Cardiol 42, 861–868.

Hirosumi J, Tuncman G, Chang L. 2002.  A central role for JNK in obesity and insulin resistance. Nature 420, 333-6.

Hotamisligil GS, Shargill NS, Spiegelman BM. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259, 87-91.

Hotamisligil GS. 2006. Inflammation and metabolic disorders. Nature 444, 860-7.

Huang H, Iida KT, Sone H, Ajisaka R. 2007. The regulation of adiponectin receptors expression by acute exercise in mice. Exp Clin Endocrinol Diabetes 115(7), 417-22.

Jakobsen SN, Hardie DG, Morrice N, Tornqvist HE. 2001. 5 -AMP-activated protein kinase phosphorylates IRS-1 on Ser-789 in mouse C2C12 myotubes in response to 5-aminoimidazole-4-carboxamide riboside. J Biol Chem 276, 46912– 46916.

Julia W, Karen C, Javier R, Ascension M. 2010. Role of physical activity on immune function Physical activity, exercise and low-grade systemic inflammation. Proceedings of the Nutrition Society 69, 400–406.

Jurimae J, Jurimae T.2005. Leptin responses to short term exercise in college level male rowers. Br J Sports Med 39, 6–9.

Jurimae J, Hofmann P, Jurimae T. 2006. Plasma adiponectin response to sculling exercise at individual anaerobic threshold in college level male rowers. Int J Sports Med 27, 272–7.

Kern PA, Saghizadeh M, Ong JM, Bosch RJ, Deem R, Simsolo RB. 1995. The expression of tumor necrosis factor in human adipose tissue. Regulation by obesity, weight loss, and relationship to lipoprotein lipase. J Clin Invest 95, 2111–9.

Kim SK, Jung I, Kim JH. 2008. Exercise reduces C-reactive protein and improves physical function in automotive workers with low back pain. J Occup Rehabil 18, 218–22.

Kony S, Zureik M, Driss F. 2004. Association of BHR and lung function with CRP: a population based study. Thorax 59, 1-5.

Kutasy B, Laxamanadass G, Puri P. 2010. Is C-reactive protein a reliable test for suspected appendicitis in extremely obese children? Pediatr Surg Int 26(1), 123-5.

Lang CH, Dobrescu C, Bagby GJ. 1992. Tumor necrosis factor impairs insulin action on peripheral glucose disposal and hepatic glucose output. Endocrinology 130, 43-52.

Mendall MA, Strachan DP, Butland BK. 2000. C-reactive protein: relation to total mortality, cardiovascular mortality and cardiovascular risk factors in men. Eur Heart J 21, 1584-90.

Moschen AR, Molnar C, Geiger S, Graziadei I, Ebenbichler CF, Weiss H et al. 2010. Anti-inflammatory effects of excessive weight loss: potent suppression of adipose interleukin 6 and tumour necrosis factor {alpha} expression. Gut 59(9), 1259-64.

Mullis R, Campbell IT, Wearden AJ, Morriss RK, Pearson DJ. 1999. Prediction of peak oxygen uptake in chronic fatigue syndrome. British Journal of Sports Medicine 33(5), 352-6.

Panagiotakos DB, Pitsavos C, Chrysohoou C, Skoumas E, Stefanadis C. 2008. Five-year incidence of ardiovascular disease and its predictors in Greece: the ATTICA study. Vasc Med 13, 113–21.

Petersen AM, Pedersen BK. 2006. The role of IL-6 in mediating the anti-inflammatory effects of exercise. J Physiol Pharmacol 57(10), 43-51.

Rotter V, Nagaev I, Smith U. 2003. Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J Biol Chem 278, 45777-84.

Saghizadeh M, Ong JM, Garvey WT, Henry RR, Kern PA. 1996. The expression of TNF alpha by human muscle. Relationship to insulin resistance. J Clin Invest 97, 1111–1116.

Sahoo RC, Acharya PR, Noushad TH, Anand R, Acharya VK, Sahu KR. 2009. A Study of High-Sensitivity C-Reactive Protein in Bronchial Asthma. Indian journal of chest diseases & allied sciences 51(4), 213-6.

Sharman MJ, Volek JS. 2004. Weight loss leads to reductions in inflammatory biomarkers after a very-low-carbohydrate diet and a low-fat diet in overweight men. Clin Sci (Lond) 107, 365–9.

Sherman CB. 1991. Health effects of cigarette smoking. Clin Chest Med 12, 643-58.

Sheu WH, Chang TM, Lee WJ, Ou HC, Wu CM, Tseng LN et al. 2008. Effect of weight loss on proinflammatory state of mononuclear cells in obese women. Obesity (Silver Spring) 16(5), 1033-8.

Tilg H, Moschen AR. 2006. Adipocytokines: mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol 6, 772-83.

Valsamakis G, McTernan PG, Chetty R, Al Daghri N, Field A, Hanif W et al. 2004. Modest weight loss and reduction in waist circumference after medical treatment are associated with favorable changes in serum adipocytokines. Metabolism 53(4), 430-4.

Zureik  M, Benetos A, Neukirch C. 2011. Reduced pulmonary function is associated with central arterial stiffness in men. Am J Respir Crit Care Med 164, 2181-5.