Identification and characterization of at CNGC19 for its role in salt stress regulation in Arabidopsis thaliana
Paper Details
Identification and characterization of at CNGC19 for its role in salt stress regulation in Arabidopsis thaliana
Abstract
Cyclic nucleotide gated ion channels (CNGCs) in plants, animals and prokaryotes have very important role in signaling and development. Structural analysis of cyclic nucleotide gated ion channels showed that CNGC 4, 5, 6 and 9 had long untranslated introns, while CNGC 7, 8, 13 and 16 had fully translated exons and introns. The insertion was confirmed in STC13 on chromosome 3 through TAIL PCR and the expression of AtCNGC19 was determined by RT PCR, which was activated twice in control and about 5 times under 150mM NaCl. The expression of gene was significantly reduced in knock out lines. The over expression lines were generated for expressing AtCNGC19 under 35: S constitutive promoter. The calli showed salt tolerance at 150mM NaCl compared with control. AtCNGC19 showed significant enhancement under salt stress in microarray. CNGCs expression under abiotic stresses showed that CNGC 19 of group IVa was highly expressed in roots under salt stress after 6h. These results provide new evidences for role of AtCNGC19 undersalt stress regulation in Arabidopsis thaliana. The study for them explores new insights of AtCNGCs helpful for functional genomics of CNGCs in plants and for their roles during biotic and abiotic stress.
Bartels D, Sunkar R. 2005. Drought and salt tolerance in plants. Critical Review of Plant Science 24, 23-58.
Bridges D, Fraser ME, Moorhead GB. 2005. Cyclic nucleotide binding proteins in the Arabidopsis thaliana and Oryza sativa genomes. BMC Bioinformatics 6, 6. DOI: 10.1186/1471-2105-6-6.
Blume B, Nurnberger T, Nass N, Scheel D. 2000. Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley. The Plant Cell 12, 1425-1440.
Chan CWM, Schorrak LM, Smith RK, Bent AF, Sussman MR. 2003. A cyclic nucleotide-gated ion channel, CNGC2, is crucial for plant development and adaptation to calcium stress. Plant Physiology 132, 728-731.
De Silva K, Laska B, Brown C, Sederoff HW, Khodakovskaya M. 2011. Arabidopsis thaliana calcium-dependent lipid binding protein (AtCLB): a novel repressor of abiotic stress response. Journal of Experimental Botany 62, 2679-2689
Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783-791.
Gao X, Tsang JC, Gaba F, Wu D, Lu L, Liu P. 2014. Comparison of TALE designer transcription factors and the CRISPR/Cas9 in regulation of gene expression by targeting enhancers. Nucleic Acids Research 42, 155-155.
Hasanuzzaman M, Nahar K, Alam MM, Bhowmik PC, Hossain MA, Rahman MM, Fujita M. 2014. Potential use of halophytes to remediate saline soils. Biomed Res Int, 2014, 589341. DOI: 10.1155/2014/589341
Kohler C, Neuhaus G. 2000. Characterisation of calmodulin binding to cyclic nucleotide‐gated ion channels from Arabidopsis thaliana. Febs Letters 471, 133-136.
Leng Q, Mercier RW, Hua BG, Fromm H, Berkowitz GA. 2002. Electrophysiological analysis of cloned cyclic nucleotide-gated ion channels. Plant Physiology 128, 400-410.
Li W, Liu B, Yu L, Feng D, Wang H, Wang J. 2009. Phylogenetic analysis, structural evolution and functional divergence of the 12-oxo-phytodienoate acid reductase gene family in plants. BMC Evol. Biol 9, 90. DOI: 10.1186/1471-2148-9-90
Luan S. 2008. The CBL – CIPK network in plant calcium signaling, Trends in Plant Science 14, 37-42.
Ma W, Yoshioka K, Berkowitz G. 2006. Cyclic nucleotide gated channels and Ca2+-mediated signal transduction during plant innate immune response to pathogens. Plant Signal. Behav 2, 548-550.
Maser PS, Thomine JI, Schroeder JM, Ward JM, Hirschi K, Sze H, Harper JF. 2001. Phylogenetic relationships within cation transporter families of Arabidopsis. Plant Physiology 126, 1646-1667.
Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Anuual Review of Plant Biology 59, 651-681.
Nawaz F, Ashraf MY, Ahmad R, Waraich E A, Shabbir RN. 2014. Selenium (Se) Regulates Seedling Growth in Wheat under Drought Stress. Advances in Chemistry 2014: 143567.
Saand, MA, Xu YP, Munyampundu JP, Li W, Zhang XR, Cai XZ. 2015. Phylogeny and evolution of plant cyclic nucleotide-gated ion channel (CNGC) gene family and functional analyses of tomato CNGCs. DNA Research 22, 471-483.
Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4, 406-425.
Shao HB, Chu LH, Shao MA, Li SQ, Yao JC. 2008. Bioengineering plant resistance to abiotic stresses by the global calcium signal system. Biotechnology Advances 26, 503-510.
Talke IN, Blaudez D, Maathuis FJ, Sanders D. 2003. CNGCs: prime targets of plant cyclic nucleotide signalling. Trends in plant science 8, 286-293.
Thompson JD, Higgins DG, Gibson TJ, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.
Wang YF, Munemasa S, Nishimura N. 2013. Identification of cyclic GMP-activated nonselective Ca2+-permeable cation channels and associate CNGC5 and CNGC6 genes in Arabidopsis guard cells, Plant Physiol 163, 578-90.
Zhang X R, Xu YP, Cai XZ. 2018. SlCNGC1 and SlCNGC14 suppress Xanthomonas oryzae pv. oryzicola-induced hypersensitive response and non-host resistance in tomato. Frontier in Plant Science 9, 285.
Sadaf Oranab, Bushra Munir, Khadim Hussain, Aftab Ahmad, Abdul Ghaffar, 2019. Identification and characterization of at CNGC19 for its role in salt stress regulation in Arabidopsis thaliana. Int. J. Biosci., 14(4), 377-385.
Copyright © 2019 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.