Identification and cloning of PIP1 gene in carrizo citrange (Citrus sinensis × Citrus trifoliate)

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Research Paper 01/10/2015
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Identification and cloning of PIP1 gene in carrizo citrange (Citrus sinensis × Citrus trifoliate)

E. Gholivandan, M.R. Dadpoor, A. Movafeghi, F. Zaree Nahandi, D. Zare Haghi, M. Kosari-Nasab
J. Bio. Env. Sci.7( 4), 16-22, October 2015.
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Abstract

The genus Citrus (Rutaceae) is economically very important and is grown in tropical and subtropical areas of the world. One of the most important factors in raisingthe quantity and quality of citrus production is sufficient water. Aquaporinsare integral membrane pore proteins and conduct water molecules in and out of the cell. Plant aquaporins divided into four subgroups including plasma membrane intrinsic proteins (PIPs), tonoplast membraneintrinsic proteins (TIPs), nodulin-26–like intrinsic proteins (NIPs) and small basic intrinsicproteins (SIPs). The present study was carried out for identification of PIP1 gene in Carrizo citrange (Citrus sinensis×Citrus trifoliate). From results, a fragment with 867- bais-pair (bp) lengthwith high similarity to PIP1gene in plants was identified as a probable member of the PIP gene family. The cloned cDNA sequence has been submitted to Gen Bank under the accession number KJ546461.1.This gene is encoding a deduced protein containing 288 amino acids. The three-dimensional structural model of the protein was also constructed by SWISS-MODEL server, indicating that the gene structure of PIPs has been highly conserved.

VIEWS 5

Agre P, Bonhivers M, Borgnia MJ. 1998. The aquaporins, blueprints for cellular plumbing systems. Journal of Biological Chemistry 273, 14659-14662.

Agustí M, Mesejo C, Reig C, Martínez-Fuentes A. 2014. Citrus Production. In: Dixon GR, Aldous DE (eds) Horticulture: Plants for People and Places, Volume 1. Springer Netherlands, 159-195.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. Journal of molecular biology 215, 403-410.

Arbona V, Iglesias D, Jacas J, Primo-Millo E, Talon  M,  Gómez-Cadenas  A.  2005.  Hydrogel substrate amendment alleviates drought effects on young citrus plants. Plant Soil 270, 73-82.

Aroca R, Ferrante A, Vernieri P, Chrispeels MJ. 2006. Drought, abscisic acid and transpiration rate effects on the regulation of PIP aquaporin gene expression and abundance in phaseolus vulgaris plants. Annals of Botany 98, 1301-1310.

Boursiac Y, Chen S, Luu D-T, Sorieul M, van den Dries N, Maurel C. 2005. Early Effects of Salinity on Water Transport in Arabidopsis Roots. Molecular and Cellular Features of Aquaporin Expression. Plant Physiology 139, 790-805.

Bray EA. 2001. plant response to water-deficit stress. In: eLS. John Wiley & Sons, Ltd.

Cheng FS, Roose ML. 1995. Origin and inheritance of dwarfing by the citrus rootstock Poncirus trifoliata `Flying dragon’. Journal of the American Society for Horticultural Science 120, 286-291.

Chung CT, Niemela SL, Miller RH. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proceedings of the National Academy of Sciences of the United States of America 86, 2172-2175.

Cohen S, Moreshet S, Guillou LL, Simon JC, Cohen M. 1997. response of citrus trees to modified radiation regime in semi-arid conditions. Journal of experimental botany 48, 35-44.

Danielson JA, Johanson U. 2008. Unexpected complexity of the aquaporin gene family in the moss Physcomitrella patens. BMC Plant Biology 845-48.

Fouquet R, Leon C, Ollat N, Barrieu F. 2008. Identification of grapevine aquaporins and expression analysis in developing berries. Plant cell reports 27, 1541-1550.

García-Sánchez F, Syvertsen JP, Gimeno V, Botía P, Perez-Perez JG. 2007. Responses to flooding and drought stress by two citrus rootstock seedlings with different water-use efficiency. Physiologia plantarum 130, 532-542.

Hachez C, Zelazny E, Chaumont F. 2006. Modulating the expression of aquaporin genes in planta: A key to understand their physiological functions. Biochimica et biophysica acta 1758, 1142-1156.

Ishikawa F, Suga S, Uemura T, Sato MH, Maeshima M. 2005. Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS letters 579, 5814-5820.

Javot H, Lauvergeat V, Santoni V, Martin-Laurent F, Guclu J, Vinh J, Heyes J, Franck KI, Schaffner AR, Bouchez D, Maurel C. 2003.Role of a single aquaporin isoform in root water uptake. The Plant cell 15, 509-522.

Javot H, Maurel C. 2002. The role of aquaporins in root water uptake. Annals of Botany 90, 301-313.

Johanson U, Karlsson M, Johansson I, Gustavsson S, Sjovall S, Fraysse L, Weig AR, Kjellbom P. 2001. The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiology, 126, 1358-1369.

Johansson I, Karlsson M, Johanson U, Larsson C, Kjellbom P. 2000. The role of aquaporins in cellular and whole plant water balance. Biochimica et biophysica acta 1465, 324-342.

Maurel C, Verdoucq L, Luu DT, Santoni V. 2008. Plant aquaporins: membrane channels with multiple integrated functions. Annual review of plant biology 59, 595-624.

Quigley F, Rosenberg JM, Shachar-Hill Y, Bohnert HJ. 2002. From genome to function: the Arabidopsis aquaporins. Genome Biology.

Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M. 2005. Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant & cell physiology 46, 1568-1577.

Wallace IS, Choi WG, Roberts DM. 2006. The structure, function and regulation of the nodulin 26-like intrinsic protein family of plant aquaglyceroporins. Biochimica et biophysica acta, 1758, 1165-1175.