Identification and cloning of PIP1 gene in carrizo citrange (Citrus sinensis × Citrus trifoliate)

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Research Paper 01/10/2015
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Identification and cloning of PIP1 gene in carrizo citrange (Citrus sinensis × Citrus trifoliate)

E. Gholivandan, M.R. Dadpoor, A. Movafeghi, F. Zaree Nahandi, D. Zare Haghi, M. Kosari-Nasab
J. Bio. Env. Sci.7( 4), 16-22, October 2015.
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The genus Citrus (Rutaceae) is economically very important and is grown in tropical and subtropical areas of the world. One of the most important factors in raisingthe quantity and quality of citrus production is sufficient water. Aquaporinsare integral membrane pore proteins and conduct water molecules in and out of the cell. Plant aquaporins divided into four subgroups including plasma membrane intrinsic proteins (PIPs), tonoplast membraneintrinsic proteins (TIPs), nodulin-26–like intrinsic proteins (NIPs) and small basic intrinsicproteins (SIPs). The present study was carried out for identification of PIP1 gene in Carrizo citrange (Citrus sinensis×Citrus trifoliate). From results, a fragment with 867- bais-pair (bp) lengthwith high similarity to PIP1gene in plants was identified as a probable member of the PIP gene family. The cloned cDNA sequence has been submitted to Gen Bank under the accession number KJ546461.1.This gene is encoding a deduced protein containing 288 amino acids. The three-dimensional structural model of the protein was also constructed by SWISS-MODEL server, indicating that the gene structure of PIPs has been highly conserved.


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