Identification and cloning of PIP2-like gene in Poncirus trifoliata and Swingle citrumelo
Paper Details
Identification and cloning of PIP2-like gene in Poncirus trifoliata and Swingle citrumelo
Abstract
Water transport through cellular membranes is facilitating by water channels, the so-called aquaporins. Aquaporins were shown to be involved in many physiological processes such as root water uptake, reproduction or photosynthesis. There are some evidences confirming that PIP proteins could regulate the whole water transport through plant tissues. This study is aimed to isolate the PIP2 gene in Poncirus trifoliata and Swingle citromelo. The fragment of this gene was amplified by polymerase chain reaction and then was cloned in a TA vector. The sequence of PIP2 gene exhibited a high similarity with the other plant PIP2 genes in the NCBI gene bank. The results indicate that this gene sequence owns a higher identity (97%) with Citrus sinensis PIP2-1-like gene that belongs to the same taxonomic family with trifoliate orange (Rutaceae).
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. Journal of Molecular Biology 215, 403-410.
Bañuls J, Primo-Millo E. 1995. Effects of salinity on some citrus scion-rootstock combinations. Annals of Botany 76, 97-102.
Barone LM, Shih C, Wasserman BP. 1997. Mercury-induced Conformational Changes and Identification of Conserved Surface Loops in Plasma Membrane Aquaporins from Higher Plants TOPOLOGY OF PMIP31 FROM BETA VULGARIS L. Journal of Biological Chemistry 272, 30672-30677.
Boursiac Y, Chen S, Luu DT, Sorieul M, van den Dries N, Maurel C. 2005. Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant Physiology 139, 790-805.
Chung C, Niemela SL, Miller RH. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proceedings of the National Academy of Sciences 86, 2172-2175.
Galmes J, Pou A, Alsina MM, Tomas M, Medrano H, Flexas J. 2007. Aquaporin expression in response to different water stress intensities and recovery in Richter-110 (Vitis sp.): relationship with ecophysiological status. Planta 226, 671-681.
Gong X-Q, Liu J-H. 2013. Genetic transformation and genes for resistance to abiotic and biotic stresses in Citrus and its related genera. Plant Cell, Tissue and Organ Culture (PCTOC) 113, 137-147.
Hasegawa PM, Bressan RA, Zhu J-K, Bohnert HJ. 2000. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51, 463-499.
Johanson U, Karlsson M, Johansson I, Gustavsson S, Sjövall S, Fraysse L, Weig AR, Kjellbom P. 2001. The Complete Set of Genes Encoding Major Intrinsic Proteins in Arabidopsis Provides a Framework for a New Nomenclature for Major Intrinsic Proteins in Plants. Plant Physiology 126, 1358-1369.
Kaldenhoff R, Fischer M. 2006. Aquaporins in plants. Acta Physiology (Oxf) 187, 169-176.
Kaldenhoff R, Ribas-Carbo M, Sans JF, Lovisolo C, Heckwolf M, Uehlein N. 2008. Aquaporins and plant water balance. Plant, Cell & Environment 31, 658-666.
King LS, Kozono D, Agre P. 2004. From structure to disease: the evolving tale of aquaporin biology. Nature Reviews Molecular Cell Biology 5, 687-698.
Lovisolo C, Secchi F, Nardini A, Salleo S, Buffa R, Schubert A. 2007. Expression of PIP1 and PIP2 aquaporins is enhanced in olive dwarf genotypes and is related to root and leaf hydraulic conductance. Physiologia Plantarum 130, 543-551.
Martre P, Morillon R, Barrieu F, North GB, Nobel PS, Chrispeels MJ. 2002. Plasma membrane aquaporins play a significant role during recovery from water deficit. Plant Physiology 130, 2101-2110.
Maurel C, Verdoucq L, Luu DT, Santoni V. 2008. Plant aquaporins: membrane channels with multiple integrated functions. Annual Review of Plant Biology 59, 595-624.
Secchi F, Lovisolo C, Schubert A. 2007. Expression of OePIP2. 1 aquaporin gene and water relations of Olea europaea twigs during drought stress and recovery. Annals of applied biology 150, 163-167.
Steudle E, Frensch J. 1996. Water transport in plants: role of the apoplast. Plant and Soil 187, 67-79.
Sui H, Han B-G, Lee JK, Walian P, Jap BK. 2001. Structural basis of water-specific transport through the AQP1 water channel. Nature 414, 872-878.
Tournaire-Roux C, Sutka M, Javot H, Gout E, Gerbeau P, Luu D-T, Bligny R, Maurel C. 2003. Cytosolic pH regulates root water transport during anoxic stress through gating of aquaporins. Nature 425, 393-397.
Tyerman SD, Niemietz CM, Bramley H. 2002. Plant aquaporins: multifunctional water and solute channels with expanding roles. Plant, Cell & Environment 25, 173-194.
M. Yusefi, M.R. Dadpoor, A. Movafeghi, F. Zaree Nahandi, D. Zare Haghi, M. Kosari-Nasab (2015), Identification and cloning of PIP2-like gene in Poncirus trifoliata and Swingle citrumelo; JBES, V7, N1, July, P440-445
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