Identification and cloning of PIP2-like gene in Poncirus trifoliata and Swingle citrumelo

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Research Paper 01/07/2015
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Identification and cloning of PIP2-like gene in Poncirus trifoliata and Swingle citrumelo

M. Yusefi, M.R. Dadpoor, A. Movafeghi, F. Zaree Nahandi, D. Zare Haghi, M. Kosari-Nasab
J. Bio. Env. Sci.7( 1), 440-445, July 2015.
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Water transport through cellular membranes is facilitating by water channels, the so-called aquaporins. Aquaporins were shown to be involved in many physiological processes such as root water uptake, reproduction or photosynthesis. There are some evidences confirming that PIP proteins could regulate the whole water transport through plant tissues. This study is aimed to isolate the PIP2 gene in Poncirus trifoliata and Swingle citromelo. The fragment of this gene was amplified by polymerase chain reaction and then was cloned in a TA vector. The sequence of PIP2 gene exhibited a high similarity with the other plant PIP2 genes in the NCBI gene bank. The results indicate that this gene sequence owns a higher identity (97%) with Citrus sinensis PIP2-1-like gene that belongs to the same taxonomic family with trifoliate orange (Rutaceae).


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