Identification and Study of cis regulatory elements and phylogenetic relationship of TaSRG and other salt response genes

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Research Paper 01/07/2014
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Identification and Study of cis regulatory elements and phylogenetic relationship of TaSRG and other salt response genes

Sirvan Saeediazar, Hamid Najafi Zarrini, Gholamali Ranjbar, Parviz Heidari
J. Bio. Env. Sci.5( 1), 1-5, July 2014.
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Salt stress is the major limiting factor in agriculture that affects on all developmental stages including germination, seedling and vegetative stages. Under salt stress, induce the expression of many plant genes. These genes encode the defending proteins, and regulatory proteins that regulate the stress signal transduction. The TFs interact with cis-elements in the promoter regions of several stress-related genes and thus up-regulate the expression of many downstream genes resulting in imparting abiotic stress tolerance. The identification of cis-regulatory DNA elements responsive to stress is important for studying gene regulation and for biotechnological applications. In this study, we identified cis-regulatory elements and also investigated the phylogenetic relationship of salt response genes of wheat, rice, barley, maize and Arabidopsis. The result of phylogenetic tree showed that TaSRG, LTP and IPK2 genes have located in one branch. The analysis of 5´UTR of salt response genes showed four elements have the most frequencies that including, TATA Box (24.55%), Unnamed-4 (24.55%), CAAT Box (17.43%) and SP1(5.33%), respectively. The TaSRG gene also is a cis elements-rich gene in which TATA Box (5′-TATAAA-3′) and CAAT (5′-CAAT-3′) Box have most frequencies. The most important identified cis regulatory elements in response to stress are ABRE, G-box, MBS, TGA-element. This results indicated that gene expression control in different checkpoints of cell cycle may be dependent to both the frequency of enhancers and stress responsive cis regulatory elements under abnormal conditions.


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