Paper Details

Identification of antagonist molds against Pyricularia oryzae using internal transcribed spacers (ITS)

Hellen Aulia Putri, Suharjono, Luqman Qurata Aini

DOI: https://dx.doi.org/10.12692/ijb/4.10.154-158

Int. J. Biosci. 4(10), 154-158. May, 2014. (PDF)

Abstract:

There are several troubles that found in rice production of national program, for example is blast disease that caused by Pyricularia oryzae. One of problem solving to control this disease is using biocontrol agent which more ecofriendly and specific target, such antagonist molds. Identification of antagonist molds using ITS5 and ITS4 universal primary was to completing identification using phenotype characteristics. This primary will amplify ribosomal cassette (partial sequence of 18S rDNA, complete sequence of 5.8S rDNA and partial sequence of 28S rDNA also complete sequence of ITS1 and ITS2). Molecular analysis using ITS5 and ITS4 was applied on every antagonist molds, including DNA isolation, amplification, purification, sequencing and alignment. The result showed that five isolates TBd 11, TBd 9, TG 2.1, TG 4.1 and TG 6 same as Trichoderma Genus. Isolates TBd 11, TBd 9 dan TG 2.1 same as Trichoderma asperellum with similarity value 100 %, isolate TG 4.1 same as Trichoderma hamatum with similarity value 94,96 % and TG 6 same asTrichoderma viride with similarity value 98,42 %. In contrast, isolate TBd 6 formed another clade with Genus Rhizomucor, Rhizomucor variabilis species with similarity value 100 %.