Identification of most effective sterilization protocol(S) for establishment of in vitro culture of endangered medicinal plant Skimmia laureola

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Research Paper 01/02/2018
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Identification of most effective sterilization protocol(S) for establishment of in vitro culture of endangered medicinal plant Skimmia laureola

Samina Siddique, Zishan Gul, Zafar Jamal, Asif ur Rehman
Int. J. Biosci.12( 2), 198-208, February 2018.
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Skimmia laureola (DC) is an important endangered medicinal plant. The present research was conducted to investigate the effect of sterilizing procedure(s) for establishment of in vitro culture of Skimmia laureola. The explants were cultured on MS medium containing coconut water as growth promoter instead of any synthetic plant growth hormone. Mercuric chloride (HgCl2) at 0.5 %concentration for 30 seconds and 1 minute was found effective sterilization procedure when used alone or in combination with fungicide (Ridomil) where 100 % contamination was controlled with 100 % plants survived. Sodium hypochlorite (NaOCl) alone and with fungicide at both concentrations i.e 3% and 5% remained less effective, however, NaOCl + fungicide at 3% concentration for 1 minute showed 20% contamination with 80% survived explants. After 28 days the survived plantlets in HgCl2 treatment showed average 3.26 cm shoot length with average 5 number of leaves per explant. It has been concluded that HgCl2 can be used as sterilant for establishment of Skimmia laureola in vitro cultures and further work is needed for standardizing  HgCl2 concentrations and time of exposure  for sterilization of  other endangered medicinal plants.


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