Identification of pi-a gene for resistance to blast in rice (Oryza sativa L.) cultivated in Mansehra

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Research Paper 01/11/2017
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Identification of pi-a gene for resistance to blast in rice (Oryza sativa L.) cultivated in Mansehra

Aqib Zeb, Abdullah Khan, Sajid Fiaz, Farkhanda Nizam, Rizwan Khan, Farrukh Shabbir, Muhammad Farooq
Int. J. Biosci.11( 5), 220-227, November 2017.
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A molecular survey was conducted for the screening of Pi-a blast resistance gene advance lines of rice developed by Dr. Fida Muhammad Abbasi, Professor at Department of Agriculture, Hazara University Mansehra. Sequence Tag Site (STS) marker Yac72 was used in this study that amplified 905 bp for presence of Pi-a gene only in advance lines of rice respectively. These advance lines were further restricted by Hinf-1 enzyme which further differentiated the resistance and susceptible advance lines which showed degrees of resistance to the causal organism of blast (M. grisea) ranging from highly resistant to susceptible. After digestion of these fragments with Hinf-1, a 905-bp was cut into two fragments of ~635 bp and ~270 bp. The lines with restricted fragments of 635 bp were considered as the lines with Pi-a blast resistance gene. Among cultivated varieties Basmati-385 and Jp-5 shows the presence of pi-a gene having 635 bp and 270 bp fragments each when restricted with Hinf-1. 9 advance lines show having fragments of 635bp and 270bp indicating the presence of Pi-a blast resistance gene while 11 lines were lacking this gene. Grain length of genotypes was also measured that ranges from 4.68 to 7.98 mm. On the basis of grain length the genotypes were categorized into short, medium, long and extra-long. In this study 6 advance lines possessed extra-long, 12 long, 1 medium and only 1 have short grains.


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