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Identification of stripe rust (Puccinia striiformis) resistant genes among Pakistani spring wheat by using molecular markers

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Research Paper 01/10/2017
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Identification of stripe rust (Puccinia striiformis) resistant genes among Pakistani spring wheat by using molecular markers

Muhammad Fayyaz, Armghan Shahzad, Ghulam Muhammad Ali, Atiq ur Rehman Rattu, Fida Muhammad
Int. J. Biosci.11( 4), 320-334, October 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: DNA markersGene postulationMarkers assisted selectionStripe rustWheat

Abstract

The present study was designed to identify the stripe rust resistant genes in Pakistani wheat by using molecular markers. First of all, one hundred and twenty five wheat varieties/lines (will be further named as genotypes) were screened under glass house and field conditions against a dominant Pakistani stripe rust race 574232. Sixty eight wheat genotypes exhibited low infection type and only two genotypes showed high infection type while all other genotypes were moderately resistant to moderately susceptible under both situations. Twelve pairs of stripe rust linked microsatellite and sequence tag site markers were used to identify Yr genes in wheat genotypes. Stripe rust resistant gene Yr 5 was found in 36.8% and 62.4% wheat genotypes through S19M97 and S23M41 markers, respectively. The marker Iag95 identified Yr 9 in 27.2% wheat genotypes and marker Psp 3000 identified Yr 10 in 87.2% genotypes. Yr 17 was found in 34.4% genotypes through Venturip-LN2. Yr 18 was found in 24.8% and 11.2% genotypes by marker CsLV 34 and Cssfr 5, respectively. Yr 26 linked Xwmc 419 and Xgwm 11 showed amplification in 98.4% and 82.4% genotypes, respectively while Yr 29 linked Xwmc 367 showed amplification in 91.2% wheat genotypes. This study will help breeders for gene pyramiding in high yielding wheat genotypes to get rid of stripe rust.

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Afzal SN, Haque MI,  Ahmedani MS, Munir M, Firdous SS, Rattu AR, Ahmad I, Firdous SS. 2008. Impact of Stripe Rust on Kernal of wheat sown in rainfed areas of Pakistan. Pakistan Journal of Botany 40, 923-929.

Ali S, Gladieux P, Rahman H, Saqib MS, Fiaz M, AhmedH, Leconte M, Gautier A, Justesen AF, Hovmøller MS, Enjalbert J, de Vallavieille-Pope C. 2014. Inferring the contribution of sexual reproduction, migration and off-season survival to the temporal maintenance of microbial populations: a case study on the wheat fungal pathogen Puccinia striiformis f. sp. tritici. Molecular Ecology 23, 603-617.

Ali S, Leconte M, Rahman H, Saqib MS, Gladieux P, Enjalbert J, de Vallavieille-Pope C. 2014. A high virulence and pathotype diversity of Puccinia striiformis f.sp. tritici at its center of diversity, the Himalayan region of Pakistan. 2014. Plant Pathology 140, 275-290.

Asad MA, Wang XC, He Z. 2012. Molecular mapping of stripe rust resistance gene YrSN104 in Chinese wheat line Shaannong 104. Hereditas 149, 146-152.

Bahri B, Shah SJA, Hussain S, Leconte M, Enjalbert J de Vallavieille-Pope C. 2011. Genetic diversity of the wheat yellow rust population in Pakistan and its relationship with host resistance. Plant Pathology 60, 649-660.

Bariana HS, Brown GN, Ahmed NU, Khatkar S, Conner RL, Wellings CR, Halley S, Sharp P, Laroche A. 2002. Charecterization of Tritium vavilovii derived stripe rust resistance using genetic, cytogenetic and molecular analyses and marker assisted selection. Theoretical and Applied Genetics 104, 315-320.

Begum S, Iqbal M, Ahmed I, Fayyaz M, Shahzad A, Ali GM. 2014. Allelic variation at loci controlling stripe rust resistance in spring wheat. Journal of Genetics 93, 579-586.

Bux H, Ashraf M, Chen X, Mumtaz AS. 2011. Effective genes for resistance to stripe rust and virulence of Puccinia striiformis f. sp. tritici in Pakistan. African Journal of Biotechnology 10, 5489-5495.

Chen W, Wellings CC, Chen X, Kang ZZ, Liu T. 2014 Wheat stripe (yellow) rust caused by Puccinia striiformis f. sp. tritici. Molecular Plant Pathology 5, 433-446.

Chen XM. 2005. Epidemiology and control of stripe rust (P. striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathology 27, 314-337.

Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11-15.

Ejaz M, Iqbal M, Shahzad A, Rehman A, Ahmed I, Ali GM. 2012. Genetic variation for markers linked to stem rust resistance genes in Pakistani wheat varieties. Crop Science 52, 2638-2648.

Fayyaz M, Rattu AR, Ahmad I, Hakro AA, Kazi AM. 2008. Current status of the occurrence and distribution of wheat leaf rust (Puccinia triticina) virulence in Pakistan. Pakistan Journal of Botany 40, 887-895.

Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-Qi L, Dubcovsky J. 2003. PCR Assays for the Lr37-Yr17-Sr38 Cluster of Rust Resistance Genes and Their Use to Develop Isogenic Hard Red Spring Wheat Lines. Crop Science 43, 1839-1847.

Hovmøller MS, Walter S, Bayles RA, Hubbard A, Flath K, Sommerfeldt N, Leconte M, Czembor P, Rodriguez-Algaba J, Thach T, Hansec JG, Lassen P, Justesen AF, Ali S, de Vallavieille-Pope C. 2016. Replacement of the European wheat yellow rust population by new races from the centre of diversity in the near-Himalayan region. Plant Pathology 65, 402-411.

Ibrahim M, Shah SJA, Hussain S, Ahmad M, Farhat U. 2015. Virulence pattern of wheat yellow rust and effective resistance genes to Puccinia striiformis f. sp. tritici in Pakistan. International Journal of Development Research 5, 3651-3657.

Iqbal M, Ejaz M, Ahmed I, Shahzad A, Ali GM. 2016. Molecular genetic variation for stripe rust resistance in spring wheat. Pakistan Journal of Agricultural Sciences 53, 143-150.

Jin Y, Szabo LJ, Szabo, Carson M. 2010. Century-Old Mystery of Puccinia striiformis Life History solved with the Identification of Berberis as an Alternate Host Mycology 100, 432-435.

Juan DX, Giraud T, Vautrin C, de Vaaavielle-Popes C, Solignae M. 2002. Isolation of twelve mirosattelite, using an enrichment protocol, in the phytopathogenic fungus (Puccinia striiformis f. sp. Tritici). Molecular Ecology Notes 2, 563-565.

Lagudah ES, McFadden H, Singh RP, Huerta-Espino J, Bariana HS, Spielmeyer W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoratical Applied Genetics 114, 21-30.

Law CN. 1976. Genetic control of yellow rust resistance in T. Spelta album. Plant breeding Institute, Cambridge, Annual Report 1975, 108-109.

Loegringn WQ. 1959. Methods for recording cereal rust data USDA international spring wheat nursery.

Luo PGX, Hu Y, Ren ZL,  Zhang HY, Shu K, Yang ZJ. 2008. Allelic analysis of stripe rust resistance genes on wheat chromosome 2BS. Genome 51, 922-927.

Ma J, Zhou R, Dong Y, Wang L, Wang X, Jia J. 2001 Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgidum L. using microsatellite markers. Euphytica 120, 219-226.

Mago R, Spielmeyer W, Lawrence G, Lagudah E, Ellis J, Proyr A. 2002. Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theoretical Applied Genetics 104, 1317-1324.

McIntosh RA, Zhang P,Lagudah E, Brunner S, Keller B, Hoxla S. 2008. The inhibitor of Pm8 in certain 1BL.1RS wheat genotypes. 11th International Wheat Genetics Symposium 2008, Sydney: Sydney University Press.

McIntosh RA. 1992. Pre emptive breeding to control wheat rusts. Euphytica 63, 103-113.

McIntosh RA. 1993. Cytogenetic studies in wheat. XV. Location of rust resistance gene in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36, 476-482.

McNeal F, Konzak C, Smith EP, Tate WS, Russell TS. 1971. A uniform system for recording and processing cereal research data. USDA Agricultural Research Service Bulletin 42, 34-121.

Metzger RJ, Silbaugh BA. 1970. Inheritance of resistance to stripe rust and its association with glume colour in Triticum aestivum L. P.I. 178383. Crop Science 10, 567-568.

Mujeeb-Kazi A, Kazi AG, Dundas I, Rasheed A, Ogbonnaya F, Kishii M, Bonnett D, Wang RR-C, Xu S, Chen P. 2013. Genetic diversity for wheat improvement as a conduit to food security. Advances in Agronomy 122, 179-258.

Payne PI, Holt LM, Johnson R, Snape JW. 1986. Linkage mapping of four gene loci, Glu-B1, Gli-B1, Rg1 and Yr10 on chromosome 1B of bread wheat. Genetics Agraria 40, 231-242.

Peterson RF, Campbell AB and Hannah AE. 1948. A diagrammatic scale for estimating the rust intensity of leaves and stem of cereals. Canadian Journal of Research 26, 496-500.

Pretorius ZA, Jin Y, Bender CM, Herselman L, Prins R. 2012. Seedling resistance to stem rust race Ug99 and marker analysis for Sr2, Sr24 and Sr31 in South African wheat cultivars and lines. Euphytica 186, 15-23.

Qamar M, Dilnawaz AG, Rabbani MA, Shinwari ZK, Iqbal M. 2014. Determination of rust resistance genes in Pakistani bread wheats. Pakistan Journal of Botany 46, 613-617.

Qamar M, Dilnawaz AG, Shah AH, Wellings C, Batool F. 2008. Postulation of stripe rust resistant genes in some Australian bread wheat cultivars and their response to temperature. Pakistan Journal of Botany 40, 2573-2585.

Qamar MS, Dilnawaz GA, Asif M. 2014. Determination of levels of resistance in Pakistani bread wheat cultivars against stripe rust (Puccinia striiformis) under field conditions. African Journal of Agricultural Research 7, 5887-5897.

Robert O, Abelard C, Dedryer F. 1999. Identification of molecular markers for the detection of the yellow rust resistance gene Yr17. Molecular Breeding 5, 167-175.

Semagn K, Bjornstad A and Ndjiondjop MN. 2006. An overview of molecular marker methods for plants. African Journal of Biotechnology 5, 2540-2568.

Shah SJA, Hussain S, Ahmad M, Farhatullah, Ibrahim M. 2014. Characterization of Slow Rusting Resistance against Puccinia striiformis f. sp. tritici in Candidate and Released Bread Wheat Cultivars of Pakistan. Plant Patholology & Microbiology 5, 223. DOI: 10.4172/2157-7471.1000223.

Singh RP 1992. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82, 835-838.

Singh RP, Huerta-Espino, William HM. 2005. Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turkish Journal of Agriculture 29, 121-127.

Singh RP, Kazi AM, Huerta-Espino J. 1998. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathology 88, 890-894.

Singh RP, William HM, Huerta-Espino J, Rosewarne G. 2004. Wheat rust in Asia: meeting the challenges with old and new technologies. Proceedings of the 4th international crop science congress, Brisbane, Australia, 26 Sep–1 Oct 2004, 1-13.

Smith PH, Hadfield J, Hart NJ, Koebner RMD, Boyd LA. 2007. STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS–LRR- type resistance gene cluster. Genome 50, 259-265.

Sohail Y, Shinwari ZK, Fayyaz M, Rattu AR, Mumtaz AS. 2015. A perspective of leaf rust race fhprn and its impact on leaf rust resistance in Pakistani wheat varieties. Pakistan Journal of Botany 47, 1559-1567.

Spielmeyer W, McIntosh RA, Kolmer JA, Lagudah ES. 2005. Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theoratical Applied Genetics 111, 731-735.

Suenaga K, Singh RP, Huerta-Espino J, William HM. 2003. Micros-atellite Markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology 93, 881-890.

Sumaira R, Ahmad I, Kazi AM, Sahi GM, Mirza JI, Rehman A, Ashraf M. 2010. Virulence variation of Puccinia striiformis Westend. f. sp. tritici in Pakistan. Archives Of Phytopathology And Plant Protection 43, 875-882.

Tabassum S, Ashraf M, XM Chen. 2010. Evaluation of Pakistani wheat germplasm for stripe rust resistance using molecular marker. Science China 53, 1-12.

Wang L, Ma J, Zhou R, Wang X, Jia J. 2002. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, P.I.178383 (Triticum aestivum L.) Euphytica 124, 71-73.

William HM, Singh RP, Huerta-Espino J, Ortiz-Islas S, Hoisington D. 2003. Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93, 153-159.

Yuan C, JiangH, Wang H, Li K, Heng T, Xianbin L, Daolin F. 2012. Distribution, Frequency and Variation of Stripe Rust Resistance Loci Yr10, Lr34/Yr18 and Yr36 in Chinese Wheat Cultivars. Journal of Genetics and Genomics 39, 587-592.

Zhou X L, Han DJ, Chen XM, Gou HL, Guo SJ, Rong L, Wang QL, Huang LL, Kang ZS. 2014. Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34. Theoretical and Applied Genetics 127, 2349-2358.

Zwart RS, Thompson JP, Milgate AW, Bansal UK, Williamson PW, Raman H, Bariana HS. 2010. QTL mapping of multiple foliar disease and root-lesion nematode resistances in wheat. Molecular Breeding 26,107-124.

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