Identification of the cassava phytoplasma insect in Côte d’Ivoire

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Research Paper 13/01/2024
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Identification of the cassava phytoplasma insect in Côte d’Ivoire

Christine Ahou Kouame, Daniel Kouamé Kra, Yeyeh Marie Noël Toualy, Hortense Atta Diallo, Arsène Irié Zoro Bi
Int. J. Agron. Agri. Res.24( 1), 19-25, January 2024.
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Abstract

Disease-causing phytoplasmas are transmitted by insect. Their distribution and transmission of the diseases with which they are associated are strongly linked to the feeding habits and biology of the insect vectors and the conditions of the host plants. This study was carried out in Côte d’Ivoire, specifically in the Grand-Lahou locality (an area endemic to phytoplasma disease), with a view to identifying the insect carriers and vectors of cassava phytoplasma. Insects were collected from the leaves. Total deoxyribonucleic acid (DNA) was extracted from 150 insect specimens and indirect diagnosis was carried out by Polymerase Chain Reaction (PCR) using the universal primer pair P1/P7 followed by AwkaSR/GH813f (specific primers). The molecular tests (PCR) carried out on these insects identified a probable carrier of the specie Bemisia tabaci.

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