Relationship between gene expressions and related terpenes at three developmental stages in Artemisia annua
Paper Details
Relationship between gene expressions and related terpenes at three developmental stages in Artemisia annua
Abstract
Importance of terpenes in different aspects of human’s life, makes it necessary to determine the relationship between them and their genes just in order to fathom such relationship for further exploitation of their production process, either increasing the volume of synthesis or changing the mixture of terpene content, for exponentially demanding for these invaluable compounds. To do so, In this study some genes in 2-c-methyl-d-erythritol 4- phosphate and Mevalonate pathways namely Farnesyl diphosphate synthase, Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase and Isiopentenyl diphosphate isomeras were evaluated in three of Artemisia annua tissues including leaf, bloom and flower. Real-time PCR results of those genes compared with the information taken from GC-Mass outputs of terpenes, yields the final result of this research. Results showed, relationship between genes and terpenes was not similar in tissues. In certain tissues, these genes play a limiting role, while in others, some other downstream genes were possibly more important, still the effect of post-transcriptional modification cannot be ignored. In the end, although those investigated genes in this research are somehow crucial in terpene production, in order to make a more obvious picture of terpene synthesis, evaluation of more immediate genes to terpenes, particularly terpene synthases, and proteomics data of these genes needed to make the relationship between RNA transcrips and metabolites more reliable.
Cao X, Yin T, Miao Q, Li C, Ju X, Sun Y, Jiang J. 2012. Molecular characterization and expression analysis of a gene encoding for farnesyl diphosphate synthase from Euphorbia pekinensis Rupr. Molecular Biology Reports 39, 1487-92.
Cheng AX, Lou YG, Mao YB, Lu S, Wang LJ, Chen X. 2007. Plant terpenoids: Biosynthesis and ecological functions. Journal of Integrated Plant Biology 49, 179−186.
Gao Y, Wu Y, Xu Y, Chen Z, Li H, Yin X, Zeng Zh, Chen R. 2012. Molecular cloning, characterization and functional analysis of a new isopentenyl diphosphate isomerase gene (IPI) from Curcuma wenyujin. Journal of Medicinal Plants Research 6, 3148-3155.
Gershenzon J, McConkey ME, Croteau RB. 2000. Regulation of monoterpene accumulation in leaves of peppermint. Plant Physiology 122, 205-213.
Gupta P, Akhtar N, Tewari Sh, Sangwan R, Trivedi P. 2011. Differential expression of farnesyl diphosphate synthase gene from Withania somnifera in different chemotypes and in response to elicitors. Plant Growth Regulation 65, 93-100.
Han Min Ye J, Qiao X, Xu M, Wang B, Guo DA. 2008. Characterization of phenolic compounds in the Chinese herbal drug Artemisia annua by liquid chromatography coupled to electrospray ionization mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 47, 516–525.
Hsieh MH, Goodman HM. 2005. The Arabidopsis IspH Homolog is Involved in the Plastid Nonmevalonate Pathway of Isoprenoid Biosynthesis. Plant Physiology 138, 641-653.
Huang JZ, Cheng TC, Wen PJ, Hsieh MH, Chen FC. 2009. Molecular characterization of the Oncidium orchid HDR gene encoding 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, the last step of the methylerythritol phosphate pathway. Plant Cell Reports 28, 1475–1486.
Iijima Y, Davidovich-Rikanati R, Fridman E, Gang DR, Bar E, Lewinsohn E, Pichersky. 2004. The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of basil. Plant Physiology 136, 3724–3736.
Keeling CI, Bohlmann J. 2006. Genes, enzymes and chemicals of terpenoid diversity in the constitutive and induced defense of conifers against insects and pathogens. New Phytologist Journal 138, 641–653.
Ma Y, Yuan L, Wu B, Li X, Chen S, Lu S. 2012. Genome-wide identification and characterization of novel genes involved in terpenoid biosynthesis in Salvia miltiorrhiza. Journal of Experimental Botany 63, 2809–2823.
Munoz-Bertomeu J, Arrillaga I, Ros R, Segura J. 2006. Up-regulation of 1-deoxy-Dxylulose-5-phosphatesynthase enhances production of essential oils in transgenic spike lavender. Plant Physiology 142, 890-900.
Nagegowda DA. 2010. Plant volatile terpenoid metabolism: Biosynthetic genes, transcriptional regulation and subcellular compartmentation. FEBS Letters 584, 2965–2973.
Olofsson L, Engström A, Lundgren A, Brodelius PE. 2011. Relative expression of genes of terpene metabolism in different tissues of Artemisia annua L. BMC Plant Biology 11, 45.
Tingey DT, Manning M, Grothaus LC, Burns WF. 1980. Influence of light and temperature on monoterpene emission rates from Slash Pine. Plant Physiology 65, 797-801.
Wang Q, Pi Y, Hou R, Jiang K, Huang Z, Hsieh M, Sun X. 2008. Molecular cloning and characterization of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. Biochemistry and molecular Biology reports 29, 112-118.
Wen W, Yu R. 2011. Artemisinin biosynthesis and its regulatory enzymes: Progress and perspective. Pharmacognosy Reviews 5, 189 194.
Woerdenbag H, Bos R, Salomons M C, Hendriks H, Pras N, Malingré TH. 1993. Volatile constituents of Artemisia annua L. (Asteraceae). Flavour and Fragrance Journal, 131–137.
Zare Mehrjerdi M, Bihamta MR, Omidi M, Naghavi MR, Soltanloo H, Ranjbar M. 2013. Effects of exogenous methyl jasmonate and 2-isopentenyladenine on artemisinin production and gene expression in Artemisia annua. Turkish Journal of Botany 37, 499-505.
Zeng Q, Zhao C, Yin L, Yang R, Zeng X, Huang Y, Feng L, Yang X. 2008. Cloning of artemisinin biosynthetic cDNAs and novel ESTs and quantification of low temperature-induced gene overexpression. Science in China. Series C, Life Sciences/ Chinese Academy of sciences 51(3), 232-44.
Zwenger S, Basu C. 2008. Plant terpenoids: applications and future potentials. Biotechnology and Molecular Biology Reviews 3, 001-007.
Alaeimoghadam F, M. R. Naghavi, A. H. Hossein zadeh, M. Ranjbar, 2016. Relationship between gene expressions and related terpenes at three developmental stages in Artemisia annua. Int. J. Agron. Agric. Res., 8(1), 41-49.
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