Antibacterial activity of Trichosanthes cucumerina seed lectin and study of its structural stability by fluorescence spectroscopy
Paper Details
Antibacterial activity of Trichosanthes cucumerina seed lectin and study of its structural stability by fluorescence spectroscopy
Abstract
A Trichosanthes cucumerina seed lectin (TCSL) was purified previously that showed potent inhibitory effects against Ehrlich ascites carcinoma (EAC) cells in vivo in mice. In the present study, the lectin was treated with guanidine-HCl for 2 and 4h in the presence and absence of Ca2+ and changes in the tryptophan fluorescence shift were monitored by fluorescence spectroscopy. It was found that the lectin stability was increased in the presence of Ca2+. Although the denaturant changed the environment of tryptophan residue, it did not affect the binding sites of TCSL as red blood cells became agglutinated after the treatment with EDTA. Besides the agglutination of three pathogenic bacterial species, the lectin also partially inhibited the growth of Salmonella enteritidis and Staphylococcus aureus.
Ghanekar A, Perombelon MCM. 1980. Interactions between potato lectin and some phytobacteria in relation to potato tuber decay caused by Erwinia carotovora. Journal of Phytopathology-Phytopathologische Zeitschrift 98, 137-149. http://dx.doi.org/10.1111/j.14390434.1980.tb03726.x
Gowda NM, Goswami U, Khan MI. 2008. T-antigen binding lectin with antibacterial activity from marine invertebrate, sea cucumber (Holothuria scabra): possible involvement in differential recognition of bacteria. Journal of Invertebrate Pathology 99, 141-145. http://dx.doi.org/10.1016/j.jip.2008.04.003.
Hubert F, van Der Knaap W, Noel T, Roch P. 1996. Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph. Aquatic Living Resources 9, 115-124. http://dx.doi.org/10.1051/alr:1996015.
Kabir SR, Nabi MM, Haque A, Zaman RU, Mahmud ZH, Reza MA. 2013. Pea lectin inhibits growth of Ehrlich ascites carcinoma cells by inducing apoptosis and G2/M cell cycle arrest in vivo in mice. Phytomedicine 20, 1288-1296. http://dx.doi.org/10.1016/j.phymed.2013.06.010.
Kabir SR, Reza MA. 2014. Antibacterial activity of Kaempferia rotunda rhizome lectin and its induction of apoptosis in Ehrlich ascites carcinoma cells. Applied Biochemistry and Biotechnology 172, 2866-2876. http://dx.doi.org/10.1007/s12010-013-0720-2.
Kabir SR, Hossen MA, Zubair MA, Alom MJ, Islam MF, Hossain MA, Kimura YA. 2011a. A New Lectin from the Tuberous Rhizome of Kaempferia rotunda: Isolation, Characterization, Antibacterial and Antiproliferative Activities. Protein And Peptide Letters 18, 1140-1149.
Kabir SR, Islam MF, Alom MJ, Zubair MA, Absar N. 2012. Purification and characterization of a snake gourd seed lectin with antitumor activity against Ehrlich ascites carcinoma cells in vivo in mice. Protein And Peptide Letters 19, 360-368. http://dx.doi.org/10.2174/092986612799363154.
Kabir SR, Nabi MM, Nurujjaman M, Reza MA, Alam AHMK, Zaman RU, Khalid-Bin-Ferdaus KM, Amin R, Khan MMH, Hossain MA, Uddin MS, Mahmud ZH. 2015a. Momordica charantia seeds lectin: toxicity, bacterial agglutination and antitumor properties. Applied Biochemistry and Biotechnology 175, 2616-2628. http://dx.doi.org/10.1007/s12010-014-1449-2.
Kabir SR, Zubair MA, Nurujjaman M, Haque MA, Hasan I, Islam MF, Hossain MT, Hossain MA, Rakib MA, Alam MT, Shaha RK, Hossain MT, Kimura Y, Absar N. 2011b. Purification and characterization of a Ca2+-dependent novel lectin from Nymphaea nouchali tuber with antiproliferative activities. Bioscience Reports 31, 465-475. http://dx.doi.org/10.1042/BSR20100126.
Khatun MMG, Rabbani MG, Rahaman EHMS. 2010. Estimate of genetic diversity in snake gourd (Trichosanthes cucumerina). Bangladesh Journal of Agricultural Research 35, 95-100. http://dx.doi.org/10.3329/bjar.v35i1.5870.
Liu B, Bian HJ, Bao JK. 2010. Plant lectins: Potential antineoplastic drugs from bench to clinic. Mini-review, Cancer Letters 287, 1-12. http://dx.doi.org/10.1016/j.canlet.2009.05.013.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.
Padma P, Komath SS, Nadimpalli SK, Swamy MJ. 1999. Purification in high yield and characterization of a new galactose-specific lectin from the seeds of Trichosanthes cucumerina. Phytochemistry 50, 363-371. http://dx.doi.org/10.1016/S0031-9422(98)00544-5.
Rafiq S, Majeed R, Qazi AK, Ganai BA, Wani I, Rakhshanda S, Qurishi Y, Sharma PR, Hamid A, Masood A, Hamid R. 2013. Isolation and antiproliferative activity of Lotus corniculatus lectin towards human tumour cell lines. Phytomedicine 21, 30-38. http://dx.doi.org/10.1016/j.phymed.2013.08.005.
Sitohy M, Doheim M, Badr H. 2007. Isolation and characterization of a lectin with antifungal activity from Egyptian Pisum sativum seeds. Food Chemistry 104, 971-979. http://dx.doi.org/10.1016/j.foodchem.2007.01.026.
Tian Q, Wang W, Miao C, Peng H, Liu B, Leng F, Dai L, Chen F, Bao J. 2008. Purification, characterization and molecular cloning of a novel mannose-binding lectin from rhizomes of Ophiopogon japonicas with antiviral and antifungal activities. Plant Science 175, 877-884. http://dx.doi.org/10.1016/j.plantsci.2008.09.008.
Tunkijjanukij S, Olafsen JA. 1998. Sialic acid-binding lectin with antibacterial activity from the horse mussel: further characterization and immunolocalization. Developmental and Comparative Immunology 22, 139-350. http://dx.doi.org/10.1016/S0145-305X(98)00017-2.
Md. Golam Kibria, Md. Rezaul Karim, Imtiaj Hasan, A.K.M. Asaduzzaman, Md. Belal Uddin, Syed Rashel Kabir (2016), Antibacterial activity of Trichosanthes cucumerina seed lectin and study of its structural stability by fluorescence spectroscopy; IJB, V9, N6, December, P187-192
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