International network for natural sciences – research journal
  • mendeley icon
  • linkedin icon
  • google plus icon
  • twitter icon
  • google scholar icon
  • facebook icon

Assessing Epigenetic Variation in Tissue Culture Based Plants of Sugarcane using High Performance Liquid Chromatography Approach

By: Siddra Ijaz, Rashda Naheed, Imran Ul Haq, Faiza Hassan, Maria Hasnain

Key Words: DNA methylation, Methylcytosine, Cytosine, HPLC.

Int. J. Biosci. 14(3), 65-71, March 2019.

DOI: http://dx.doi.org/10.12692/ijb/14.3.65-71

Certification: ijb 2019 0174 [Generate Certificate]

Abstract

Tissue culture system is considered as a basis for genetic transformation studies in plants. Plant tissue culture regime could bring genetic and epigenetic variation in in vitro regenerated plants though. Cytosine methylation is an imperative factor that contributes in epigenetic variation. Therefore, in this study we investigated the epigenetic variation in in vitro regenerants of sugarcane genotypes, CPF-247 and CPF-248 by using high performance liquid chromatography (HPLC) technique. In this study, in vitro regenerated plants and field grown plants of both sugarcane genotypes were assessed and analyzed for the level of cytosine and methylcytosine. Thus calculated percent concentration of 5MeC for each sample were 0.0345% and 0.258% in field grown and in vitro regenerated plants of CPF-247 respectively as well as 0.0261%  and 4.39%  in the field grown and in vitro regenerated plants of CPF-248 respectively that showed an increase in methylcytosine concentration in in vitro regenerated plants than field grown plants. The results showed that level of methylcytosine was high in tissue culture based plants of both sugarcane genotypes. While in comparison between these genotypes revealed that degree of methylcytosine concentration is high in in vitro regenerated plants of CPF-248 than in vitro regenerated plants of CPF-247. These results indicated that dissected tissue culture with hormones may bring change in the level of DNA methylation and thereby it could be the reason of epigenetic variation, which may contribute in genotypic as well as phenotypic variations.

| Views 8 |

Assessing Epigenetic Variation in Tissue Culture Based Plants of Sugarcane using High Performance Liquid Chromatography Approach

Bardini M, Labra M, Winfield M, Sala F. 2003. Antibiotic-induced DNA methylation changes in calluses of Arabidopsis thaliana. Plant Cell, Tissue and Organ Culture 72(2), 157-162.

Bird A. 2007. Perceptions of epigenetics. Nature 447, 396-398.

Bond DM, Finnegan EJ. 2007. Passing the message on: inheritance of epigenetic traits. Trends in plant science 12(5), 211-216.

Chinnusamy V, Zhu JK. 2009. Epigenetic regulation of stress responses in plants. Current opinion in plant biology 12(2), 133-139.

Demeulemeester MAC, Van Stallen N, De Proft MP. 1999. Degree of DNA methylation in chicory (Cichorium intybus L.): influence of plant age and vernalization. Plant science 142(1), 101-108.

Fraga MF, Esteller M. 2002. DNA methylation: a profile of methods and applications. Bio techniques 33(3), 632-649.

Gao Y, Ran L, Kong Y, Jiang J, Sokolov V, Wang Y. 2014. Assessment of DNA methylation changes in tissue culture of Brassica napus. Russian journal of genetics 50(11), 1186-1191.

Grativol C, Hemerly AS, Ferreira PCG. 2012. Genetic and epigenetic regulation of stress responses in natural plant populations. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms 1819(2), 176-185.

Heinz DJ, Mee GW. 1971. Morphologic, cytogenetic, and enzymatic variation in Saccharum species hybrid clones derived from callus tissue. American journal of botany 58(3), 257-262.

Huang H, Han SS, Wang Y, Zhang XZ, Han ZH. 2012. Variations in leaf morphology and DNA methylation following in vitro culture of Malus xiaojinensis. Plant Cell, Tissue and Organ Culture (PCTOC) 111(2), 153-161.

Kaeppler SM, Kaeppler HF, Rhee Y. 2000. Epigenetic aspects of somaclonal variation in plants. Plant mol. boil. 43(2-3), 179-188.

 LoSchiavo F, Pitto L, Giuliano G, Torti G, Nuti-Ronchi V, Marazziti D, Terzi M. 1989. DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs. Theoretical and Applied Genetics 77(3), 325-331.

Lukens LN, Zhan S. 2007. The plant genome’s methylation status and response to stress: implications for plant improvement. Current opinion in plant biology 10(3), 317-322.

Miguel C, Marum L. 2011. An epigenetic view of plant cells cultured in vitro: somaclonal variation and beyond. Journal of experimental botany 62(11), 3713-3725.

Neelakandan AK, Wang K. 2012. Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications. Plant cell reports 31(4), 597-620.

Rival A, Ilbert P, Labeyrie A, Torres E, Doulbeau S, Personne A, Jaligot E. 2013. Variations in genomic DNA methylation during the long-term in vitro proliferation of oil palm embryogenic suspension cultures. Plant cell reports 32(3), 359-368.

Sahu PP, Pandey G, Sharma N, Puranik S, Muthamilarasan M, Prasad M. 2013. Epigenetic mechanisms of plant stress responses and adaptation. Plant cell reports 32(8), 1151-1159.

Steward N, Kusano T, Sano H. 2000. Expression of ZmMET1, a gene encoding a DNA methyltransferase from maize, is associated not only with DNA replication in actively proliferating cells, but also with altered DNA methylation status in cold-stressed quiescent cells. Nucleic acids research 28(17), 3250-3259.

Trejgell A, Dąbrowska G, Tretyn A. 2009. In vitro regeneration of Carlina acaulis subsp. simplex from seedling explants. Acta physiologiae plantarum 31(3), 445-453.

Vanyushin BF. 2006. DNA methylation and epigenetics. Russian Journal of Genetics 42(9), 985-997.

Wang Q M, Wang L. 2012. An evolutionary view of plant tissue culture: somaclonal variation and selection. Plant cell reports 31(9), 1535-1547.

Xu M, Li X, Korban SS. 2004. DNA-methylation alterations and exchanges during in vitro cellular differentiation in rose (Rosa hybrida L.). Theoretical and Applied Genetics 109(5), 899-910.

Siddra Ijaz, Rashda Naheed, Imran Ul Haq, Faiza Hassan, Maria Hasnain.
Assessing Epigenetic Variation in Tissue Culture Based Plants of Sugarcane using High Performance Liquid Chromatography Approach.
Int. J. Biosci. 14(3), 65-71, March 2019.
https://innspub.net/ijb/assessing-epigenetic-variation-tissue-culture-based-plants-sugarcane-using-high-performance-liquid-chromatography-approach/
Copyright © 2019
By Authors and International Network for
Natural Sciences (INNSPUB)
https://innspub.net
brand
innspub logo
english language editing
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Publish Your Article
  • CALL FOR PAPERS
    CALL FOR PAPERS
    Submit Your Article
INNSPUB on FB
Email Update