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Detection and molecular characterization of Metallo beta-lactamases producing Pseudomonas aeruginosa isolated from burn patients

By: Abdullah Zafar, Nishat Zafar, Rabia Kanwar, Fariha Mazhar, Maria Shaukat, Muhammad Sarwar, Andleeb Afzal

Key Words: Antibiotic sensitivity, Burn patients, Metallo beta lactamases, P. aeruginosa.

Int. J. Biosci. 16(3), 454-463, March 2020.

DOI: http://dx.doi.org/10.12692/ijb/16.3.454-463

Certification: ijb 2020 0061 [Generate Certificate]

Abstract

Due to drug resistance, P. aeruginosa becomes more contagious that able to cause more severe infection. Those patients with acute burn existing an immunosuppressive situation and, therefore, advanced defenselessness to diseases through nosocomial pathogens along with high mortality. The purpose of the research was isolation and identification of P. aeruginosa from burn samples and determined its antibiotic sensitivity pattern and then molecular identification of MBL genes using PCR. A total of 200 clinical samples were collected from burn patients of Allied Hospital Faisalabad. P. aeruginosa was isolated on cetrimide agar, and identification was made by gram staining and biochemical tests. All isolates checked the antibiotic sensitivity pattern. Phenotypic screening of Metallo Beta Lactamases (MBL) producing P. aeruginosa and genotypic detection of MBL genes were determined. Out of 200 samples, 46.5% were positive. All isolates exhibited high resistance against multiple antibiotics except colistin, polymyxin, and aztreonam. All of 7 strains were detected PCR positive for the OprL gene using specific primers. 35.48% isolates have shown MBLs production in the combined disk diffusion method. Phenotypically detected ten isolates positive for MBLs encoded genes as followed by blaNDM 5(50%) blaVIM 3(30%) blaIMP 2(20%) and blaSIM, blaDIM, blaSPM, blaGIM, and blaAIM were not detected. MBLs producing isolates such as Pseudomonas aeruginosa are a distressing threat in health care institutes and can be control through proper following the isolation and strict detection methods that will really helpfully in reduce serious infections and death rate among the hospitalized patients.

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Detection and molecular characterization of Metallo beta-lactamases producing Pseudomonas aeruginosa isolated from burn patients

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Abdullah Zafar, Nishat Zafar, Rabia Kanwar, Fariha Mazhar, Maria Shaukat, Muhammad Sarwar, Andleeb Afzal.
Detection and molecular characterization of Metallo beta-lactamases producing Pseudomonas aeruginosa isolated from burn patients.
Int. J. Biosci. 16(3), 454-463, March 2020.
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