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Identification of stripe rust (Puccinia striiformis) resistant genes among Pakistani spring wheat by using molecular markers

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Research Paper 01/10/2017
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Identification of stripe rust (Puccinia striiformis) resistant genes among Pakistani spring wheat by using molecular markers

Muhammad Fayyaz, Armghan Shahzad, Ghulam Muhammad Ali, Atiq ur Rehman Rattu, Fida Muhammad
Int. J. Biosci.11( 4), 320-334, October 2017.
Certificate: IJB 2017 [Generate Certificate]
Key: DNA markersGene postulationMarkers assisted selectionStripe rustWheat

Abstract

The present study was designed to identify the stripe rust resistant genes in Pakistani wheat by using molecular markers. First of all, one hundred and twenty five wheat varieties/lines (will be further named as genotypes) were screened under glass house and field conditions against a dominant Pakistani stripe rust race 574232. Sixty eight wheat genotypes exhibited low infection type and only two genotypes showed high infection type while all other genotypes were moderately resistant to moderately susceptible under both situations. Twelve pairs of stripe rust linked microsatellite and sequence tag site markers were used to identify Yr genes in wheat genotypes. Stripe rust resistant gene Yr 5 was found in 36.8% and 62.4% wheat genotypes through S19M97 and S23M41 markers, respectively. The marker Iag95 identified Yr 9 in 27.2% wheat genotypes and marker Psp 3000 identified Yr 10 in 87.2% genotypes. Yr 17 was found in 34.4% genotypes through Venturip-LN2. Yr 18 was found in 24.8% and 11.2% genotypes by marker CsLV 34 and Cssfr 5, respectively. Yr 26 linked Xwmc 419 and Xgwm 11 showed amplification in 98.4% and 82.4% genotypes, respectively while Yr 29 linked Xwmc 367 showed amplification in 91.2% wheat genotypes. This study will help breeders for gene pyramiding in high yielding wheat genotypes to get rid of stripe rust.

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