Molluscicidal activity of the aqueous extract of garlic (Allium sativum L.) bulb against golden apple snail (Pomacea canaliculata L.)

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Molluscicidal activity of the aqueous extract of garlic (Allium sativum L.) bulb against golden apple snail (Pomacea canaliculata L.)

Marchee T. Picardal, Kinberly T. Panlaan, Paula Marianne L. Castaño, Leandro G. Peña, Krisska T. Abella, Jay P. Picardal
Int. J. Biosci.13( 2), 76-88, August 2018.
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Abstract

The search for efficient, organic, and environment-friendly molluscicide that could minimize the spread of the invasive Golden Apple Snail (Pomacea canaliculata) in the Philippines is still on going. To investigate the molluscicidal activity of garlic (Allium sativum) towards the rice pest (Pomacea canaliculata), this study employed Complete Randomized Design in 3 trials (n=630 snails) to test the efficacy of Aqueous Garlic Extract [AGE] under five experimental treatments (T1=10ppm AGE; T2=8.75ppm AGE; T3=7.5ppm AGE; T4=6.25ppm AGE and T5=5.0ppm AGE) and two control groups (Positive Control=Niclosamide [Snail ShatterTM] and Negative Control= distilled H2O. After 48h experimental period, mortality data were analyzed using One Way ANOVA (p<0.05) and post hoc analysis (Tukey’s test) while Probit analysis was employed to determine toxicity level of AGE at LD50 and LD90. Results showed that there is a direct relationship between AGE and snail mortality, suggesting that all treatments exhibited molluscicidal properties. However, T1 and T2 were reported to have a comparable molluscicidal effect to that of the commercially-available molluscicide (NiclosamideTM). Toxicity level (LD50) was found to be at 4.007ppm while LD90 is 7.602ppm. As evidenced by the laboratory experiment results, this study concludes that the best AGE concentration against the target mollusk (Pomacea canaliculata) is T3=7.5ppm. For future research, this study recommends the use of similar AGE concentrations to P. canaliculata and economically important, non-target organisms (Tilapia, Oreochromis niloticus) under field trials.

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Acosta BO, Pullin RSV. 1991. Environmental impact of the golden snail (Pomacea sp.) on rice farming systems in the Philippines: Summary Report of the workshop held at the freshwater aquaculture center, Central Luzon State University, Muñoz, Nueva Ecija, Philippines, 9-10 November 1989, ICLARM Conf. Proc. 28, Manila, Philippines, p 34.

Aladesanmi A. 2007. Tetrapleura tetraptera: Molluscicidal activity and chemical constituents.  African Journal of Traditional Complementary and Alternative Medicine 4(1), 23–36.

Alam MK, Hoq MO, Uddin MS. 2016. Medicinal plant Allium sativum: A review. Journal of Medicinal Plants Studies 4(6), 72-79.

Ahmed EA, Babiker OF, Abdalla RM. 2014. Molluscicidal activity of aqueous leaf extract of Solenostemma argel (Del Hayne) on Biomphalaria pfeifferi Snails. Journal of Basic and Applied Science Research 4(1), 179-184.

Amonkar SV, Reeves EL. 1970. Mosquito control with active principle of garlic, Allium sativum. Journal of Economic Entomology. 63(4), 1172-5. https://doi.org/10.1093/jee/63.4.11.72

Barker GM. 2002. Molluscs as crop pest. Landcare Research. Hamilton New Zealand, CABI Publishing, Wallingford, UK. http://dx.doi.org/10.1079/9780851993201.0000

Borja AO. 2012. Tobacco dust as organic molluscicide. The Philippine Star Jan. 22, 2012.

Brackenbury TD, Appleton CC. 1997. A comprehensive evaluation of Agave attenuata, a candidate plant molluscicide in South Africa. Biological Sciences 68(2), 201-213.

Cagauan AG, Joshi RC. 2002. Predatory effect of freshwater fishes on golden apple snail Pomacea canaliculata Lam. in greenhouse conditions. International Rice Research Notes. International Rice Research Institute, Los Baños, Laguna, Philippines.

Cagauan AG, Ferrer EC, Muangman W. 1993. Acute toxicities of insecticides with single and two groups of active ingredients and molluscicides on golden snails (Pomacea sp.) 47-50. In: Arsenia G. Final Report: Rice-Fish Ecology (July 1992-December 1993).

Crebassa X, Caruel H, Mercier F. 2012. Use of Gleditsia saponins for controlling mollusc pests. US Patent application number: 20120321688:

Cowie RH, Hayes HA. 2012. Apple snails. In Francis RA 2012, Ed. A handbook of global freshwater species. Earthscan – Taylor and Francis, Ltd. Inc., NY, USA.

Cowie RH. 2002. Apple snails as agricultural pests: their biology, impacts and management. In: Barker GM, Ed. Molluscs as Crop Pests, Landcare Research. Hamilton New Zealand, CABI Publishing, Wallingford, UK, 145-192.

Dai L, Wang W, Dong X, Hu R, Nan X. 2011. Molluscicidal activity of cardiac glycosides from Nerium indicum against Pomacea canaliculata and its implications for the mechanisms of toxicity. Environmental Toxicology and Pharmacology 32(2), 226-32. https://doi.org/10.1016/j.etap.2011.05.007

Demetillo MT, Baguio ML, Limitares DE, Madjos GG, Abrenica-Adamat LR. 2015. Effect of Cymbopogon citratus (lemon grass) crude leaf extracts on the developmental stages of Pomacea canaliculata (golden apple snail). Advances in Environmental Sciences – International Journal of the Bioflux Society 7(3), 460-467.

Farnsworth NR, Henderson TO, Soejarto DD. 1987. Plants with potential molluscicidal activity. In Plant molluscicides: Mott, KE. Ed. 131–204, John Wiley & Sons Ltd, Chichester.

Halwart M. 1994. The golden apple snail Pomacea canaliculata in Asian rice farming systems: Present impact and future threat. International Journal of Pest Management 40(2), 199-206. https://doi.org/10.1080/09670879409371882

He P, Wang W,  Sanogo S,  Zeng X, Sun X, Lv L, Yuan D, Duan L, Wu D. 2017. Molluscicidal activity and mechanism of toxicity of a novel salicylanilide ester derivative against Biomphalaria species. Parasitology Vectors 10, 383. https://doi.org/10.1186/s13071-017-2313-3

Joshi R. 2007. Problems with the management of Golden Apple Snail, Pomacea canaliculata: An important exotic pest of rice in Asia. In: Vreysen M.J.B., Robinson A.S., Hendrichs J. (eds) Area-Wide Control of Insect Pests. Springer, Dordrecht, 257-264. http://dx.doi.org/10.1007/978-1-4020-6059-5_24

Joshi RC, Meepagala KM, Sturtz G, Caguan A, Mendoza G, Payan FE, Duke SO. 2005. Molluscicidal activity of Vulgarone B from Artemisia douglasiana (Besser) against the invasive alien mollusk pest, P. canaliculata (Lamarck). International Journal of Pest Management 51, 175-180. https://doi.org/10.1080/09670870500183161

Joshi S, Bharucha C, Desai AJ. 2008. Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B. Bioresource Technology 99, 4603–4608. https://doi.org/10.1016/j.biortech.2007.07.030

Manila Bulletin [MB]. 2010. Republic Act No. 10068, otherwise known as “Organic Agriculture Act of 2010”.

Mantawy MM, Mahmoud AH. 2002. Effect of Allium cepa and Allium sativum feeding on glucose, glycogen, protein bands profile and phenol oxidase activity in Biomphalaria alexandrina. Journal of the Egyptian Society of Parasitology 32(1), 271-283.

Massaguni R, Latip SMH. 2015. Assessment of the molluscicidal properties of azadirachtin against GAS, Pomacea Canaliculata. Malaysian Journal of Analytical Science 19(4), 781-789.

Metwally NS. 2006. Potency of Allium sativum L. and Allium cepa oils against Schistosoma mansoni infection in mice. The Egyptian Journal of Hospital Medicine 23, 319-332.

Mikail HG. 2010. Phytochemical screening, elemental analysis and acute toxicity of aqueous extract of Allium sativum bulbs in experimental rabbits. Journal of Medicinal Plant Research 4(4), 322-326.

Meriga B, Moputi R, Morali T. 2012. Insecticidal, antimicrobial, and antioxidant activities of bulb extracts of Allium sativum L. Asian Pacific Journal of Tropical Medicine 5(5), 391-395. https://doi.org/10.1016/S1995-7645(12)60065-0

Mott KE. 1987. Plant Molluscicides, UNDP/World Bank/WHO, John Wiley & Sons Ltd., New York, 326.

Musman M, Kamaruzzaman S, Karina S, Rizqi R, Arisca F. 2013. A preliminary study on the anti-hatching of freshwater golden apple snail Pomacea canaliculata (Gastropoda: Ampullariidae) eggs from Barringtonia racemosa (Magnoliopsida: Lecythidaceae) seed extract. AACL Bioflux 6(4), 394-398.

Mwine J, Ssekyewa C, Kalanzi K, Van Damme P. 2013. Evaluation of selected pesticidal plant extracts against major cabbage insect pests in the field. Journal of Medicinal Plants Research 7(22), 1580-1586. http://dx.doi.org/10.5897/JMPR10.597

Nwanchukwu, Asawalam E. 2014. Evaluation of freshly prepared juice from garlic (Allium sativum L.) as a biopesticide against the maize weevil, Sitophilus zeamais (Motsch.) (Coleoptera: Curculionidae). Journal of Plant Protection Research 54(2), 132–138. https://doi.org/10.2478/jppr-2014-0021

Ngaloy IT, Andrada MG. 2005. Effect of pulverized dried bakuwog fruit on golden snail. Ecosystems Research Digest 15(2), 6-15.

Nguta J, Mbaria J, Gakuya D, Gathumbi P. 2012. Evaluation of acute toxicity of crude plant extracts from Kenyan Biodiversity using Brine Shrimp, Artemia salina L. (Artemiidae). The Open Conference Proceedings Journal 3, 30-34. http://dx.doi.org/10.2174/2210289201203010030

Pastorino G, Darrigan G. 2012. Pomacea canaliculata. The IUCN Red List of Threatened Species.

San Martin R, Cruz DO. 2013. Molluscicidal effects of saponin-rich plants extracts on the grey field slug molluscicidal effects of saponin. Clencia e Investigacion Agraria 40(2), 341-349. http://dx.doi.org/10.7764/rcia.v40i2.1102

Singh VK, Singh DK. 1995. Characterization of allicin as a molluscicidal agent in Allium sativum. Biological Agriculture and Horticulture 12(2), 119-131. https://doi.org/10.1080/01448765.1995.9754.732

Singh DK, Singh A. 1993. Allium sativum, a potent new molluscicide. Biological Agriculture and Horticulture 9(2), 121-124. https://doi.org/10.1080/01448765.1993.9754.625

Singh VK, Singh DK. 2008. Pharmacological effects of garlic (Allium sativum L.). ARBS Annual Review of Biomedical Sciences 10, 6-26. http://dx.doi.org/10.5016/1806-8774.2008.v10p6

Singh DK, Agarwal RA. 1984. Alteration of biogenic level in the snail Lymnaea acuminata by latex of Euphorbia royleana. Toxicology Letters 21(3), 309-314. https://doi.org/10.1016/0378-4274(84)90089-4

Singh DK, Agarwal RA. 1987. Latex of Euphorbia antisyphlitica, a new potent molluscicides having anticholinesterase activity against snail Lymnaea acuminata. The Science of Total Environment 61, 211-215. https://doi.org/10.1016/0048-9697(87)90368-8

Taguiling N. 2015. Effect of combined plant extracts on golden apple snail (Pomacea canaliculata) (Lam) and giant earth worm (Pheretima sp.) International Journal of Agriculture and Crop Sciences 8(1), 55-60

Tripathi SM, Singh DK. 2000. Molluscicidal activity of Punica granatum bark and Canna indica root. Brazilian Journal of Medical and Biological Research 33(11), 1351-1355. http://dx.doi.org/10.1590/S0100879X2000001100014

Tiwari F, Singh DF. 2004. Attraction to amino acids by Lymnaea acuminata, the snail host of Fasciola species. Brazilian Journal of Medical and Biological Research 37(4), 587-590. http://dx.doi.org/10.1590/S0100879X2004000400016

USDA. 2013. Giant African Cooperative Eradication Program, Environmental Assessment Supplement.

UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases. 1983.  Scientific working group on plant molluscicides. Geneva: World Health Organization. http://www.who.int/iris/handle/10665/60086